The development of a novel biohybrid material for tooth root restoration

• Project/Area Number: 22592110
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Tohoku University
• Principal Investigator: IWAMATSU-KOBAYASHI Yoko 東北大学, 歯学研究科(研究院), 助教 (50261524)
• Co-Investigator(Kenkyū-buntansha): NISHIHARA Daisuke 東北大学, 大学院歯学研究科, 非常勤講師 (10431587) ; ICHIKAWA Hiroyuki 東北大学, 大学院歯学研究科, 教授 (20193435) ; KOMATSU Masashi 東北大学, 大学院歯学研究科, 名誉教授 (10005069)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯学 / 生体材料 / 再生医学 / 細胞・組織 / 歯根修復

Research Abstract

We examined the influence of mesenchymal stem cells growth medium (MSCGM) for STRO-1 positive cells derived from PDL compared with DMEM. PDL cells cultured in MSCGM showed colony formation and the isolation rate was higher than that in DMEM. STRO-1 positive cells derived from human wisdom tooth germs collected using MSCGM showed Alcian blue positive matrix when cultured in chondrogenic induction medium. They also performed nodule formation stained with Alizarin red cultured in osteogenic induction medium.
We have designed a titanium sheet with two-dimensional porous array of 20um pored mesh which was almost same as the diameter of Sharpeys fiber and numerous holes were densely pierced. We investigated the effect of the differentiation of cells on the titanium mesh. Frios Bone Shield was used as control. The cells on the titanium mesh expressed higher osteopontin and osteocalcin expression around the pore compared with the Frios.

Research Products

• [Journal Article] The distribution of TRPV1 and TRPV2 in the rat pharynx (2013)
  • Author(s): Sasaki R., Ichikawa H. et al
  • Journal Title: Cell Mol Neurobiol Volume: 33 巻 Issue: 5 Pages: 707-714
  • DOI: 10.1007/s10571-013-9938-3
  • Peer Reviewed
• [Journal Article] Increase of CGRP-Containing Nerve Fibers in the Rat Periodontal Ligament After Luxation (2012)
  • Author(s): Nagayama T, Seiryu M, Deguchi T, Kano M, Suzuki T, Takano-Yamamoto T, Ichikawa H
  • Journal Title: Cell Mol Neurobiol Volume: 32(3) Issue: 3 Pages: 391-397
  • DOI: 10.1007/s10571-011-9767-1
  • Peer Reviewed
• [Journal Article] Masseteric nerve injury increases expression of brain derived neurotrophic factor in microgria within the rat mesencephalic trigeminal tract nucleus (2011)
  • Author(s): Ichikawa H. et al
  • Journal Title: Cell Mol Neurobiol Volume: 31巻(4) Issue: 4 Pages: 551-559
  • DOI: 10.1007/s10571-011-9648-7
  • Peer Reviewed
• [Journal Article] Pituitary adenylatecyclase-activating polypeptide-immunoreactive nerve fibers in the rat epiglottis and pharynx (2011)
  • Author(s): Kano M., Ichikawa H. et al
  • Journal Title: Ann Anat Volume: 193巻(6) Issue: 6 Pages: 494-499
  • DOI: 10.1016/j.aanat.2011.08.005
  • Peer Reviewed
• [Journal Article] Pituitary adenylatecyclase-activating polypeptide-immunoreactive nerve fibers in the rat epiglottis and pharynx (2011)
  • Author(s): Kano M., Ichikawa H., et al
  • Journal Title: Ann Anat Volume: 193(6) Pages: 494-499
  • Peer Reviewed
• [Journal Article] Masseteric nerve injury increases expression of brain-derived neurotrophic factor in microglia within the rat mesencephalic trigeminal tract nucleus (2011)
  • Author(s): Ichikawa H., et al
  • Journal Title: Cell Mol Neurobiol Volume: 31(4) Pages: 551-559
  • Peer Reviewed
• [Journal Article] Increase of c-Fos and c-Jun expression in spinal and cranial motoneurons of the degenerating muscle mouse (Scn8a(dmu)) (2010)
  • Author(s): Ichikawa H., et al
  • Journal Title: Cell Mol Neurobiol Volume: 30(5) Pages: 737-742
  • Peer Reviewed
• [Presentation] F-spondin involved in periodontal ligament differentiation through regulation of TGF-b activity (2014)
  • Author(s): Iwamatsu-Kobayashi Y. et al
  • Organizer: The 5th international symposium for interface oral health science
  • Place of Presentation: 仙台
• [Presentation] The effect of the differentiation of cultured human cells on titanium mesh (2014)
  • Author(s): Endo N., Iwamatsu-Kobayashi Y. et al
  • Organizer: The 5th international symposium for interface oral health science
  • Place of Presentation: 仙台
• [Presentation] F-spondin involved in periodontal ligament differentiation through regulation of TGF-b activity (2014)
  • Author(s): Y. Iwamatsu-Kobayashi, S. Abe, Y. Fujieda, M. Saito
  • Organizer: The 5th International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
• [Presentation] The Effect of the Differentiation of Cultured Human Cells on Titanium Mesh (2014)
  • Author(s): N. Endo, Y. Iwamatsu‐Kobayashi, N. Iwama, H. Ishihata, H. Shimauchi, M. Kikuchi
  • Organizer: The 5th International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
• [Presentation] 20μmチタンメッシュを用いた歯周組織再生 (2012)
  • Author(s): 石幡浩志、小林洋子 他
  • Organizer: 日本歯周病学会
  • Place of Presentation: 札幌
• [Presentation] 培養智歯歯胚由来細胞に対するチタンメッシュの効果 (2012)
  • Author(s): 小林洋子 他
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 広島
• [Presentation] 20μmチタンメッシュを用いた歯周組織再生 (2012)
  • Author(s): 石幡浩志、小林洋子 他
  • Organizer: 日本歯周病学会
  • Place of Presentation: 札幌コンベンションセンター、札幌市
• [Presentation] 培養智歯歯胚由来細胞に対するチタンメッシュの効果 (2012)
  • Author(s): 小林洋子 他
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 広島国際会議場、広島市
• [Presentation] 永久歯に内部吸収のみられた歌舞伎メイキャップ症候群の1例 (2011)
  • Author(s): 小林洋子, 他
  • Organizer: 第135回日本歯科保存学会2011年度秋季学術大会
  • Place of Presentation: 大阪国際交流センター、大阪
  • Year and Date: 2011-10-20
• [Presentation] Characterization of STRO-1 positive cells derived from Human Wisdom Tooth Germs (2011)
  • Author(s): Iwamatsu-Kobayashi Y., Nishihara D.,Komatsu M., et al
  • Organizer: The 4th international symposium for interface oral health science
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-08
• [Presentation] The Influence of Mesenchymal Stem Cells Growth Medium for Isolation of STRO-1 Positive Cells from Human Periodontal Ligaments (2011)
  • Author(s): Endo N., Iwamatsu-Kobayashi Y.,Nishihara D., Komatsu M., et al
  • Organizer: The 4th international symposium for interface oral health science
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-08
• [Presentation] Characterization of STRO-1 positive cells derived from Human Wisdom Tooth Germs (2011)
  • Author(s): Kobayashi-Iwamatsu Y., et al
  • Organizer: The 4^<th> International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
  • Year and Date: 2011-03-08
• [Presentation] The Influence of Mesenchymal Stem Cells Growth Medium for Isolation of STRO-1 Positive Cells from Human Periodontal Ligament (2011)
  • Author(s): Endo N., Kobayashi-Iwamatsu Y., et al
  • Organizer: The 4^<th> International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
  • Year and Date: 2011-03-08
• [Presentation] 永久歯に内部吸収のみられた歌舞伎メイキャップ症候群の1例 (2011)
  • Author(s): 小林洋子、小松正志 他
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 大阪
• [Book] Interface Oral Health Science 2011 − Characterization of STRO-1 positive cells derived from Human Wisdom Tooth Germs (2012)
  • Author(s): Iwamatsu-Kobayashi Y., Nishihara D.,Komatsu M., et al
  • Publisher: Springer社
• [Book] Interface Oral Health Science 2011−The Influence of Mesenchymal Stem Cells Growth Medium for Isolation of STRO-1Positive Cells from Human Periodontal Ligament (2012)
  • Author(s): Endo N., Iwamatsu-Kobayashi Y.,Nishihara D., Komatsu M., et al
  • Publisher: Springer社
• [Book] Interface Oral Health Science 2011-Characterization of STRO-1 positive cells derived from Human Wisdom Tooth Germs (2012)
  • Author(s): Iwamatsu-Kobayashi Y., et al
  • Publisher: Springer
• [Book] Interface Oral Health Science 2011-The Influence of Mesenchymal Stem Cells Growth Medium for Isolation of STRO-1 Positive Cells from Human Periodontal Ligament (2012)
  • Author(s): Endo N., Iwamatsu-Kobayashi Y., et al
  • Publisher: Springer