| Project/Area Number |
22592123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka University (2011-2012)
Kyushu University (2010) |
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Principal Investigator |
FUJII Shinsuke 大阪大学, 医学系研究科, 招へい研究員 (60452786)
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| Co-Investigator(Kenkyū-buntansha) |
AKAMINE Akifumi 九州大学, 歯学研究院, 教授 (00117053)
MAEDA Hidefumi 九州大学, 大学病院, 講師 (10284514)
WADA Naohisa 九州大学, 大学病院, 助教 (60380466)
TOMOKIYO Atsushi 九州大学, 大学病院, 助教 (20507777)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 歯根膜 / Wnt / 細胞外基質 / 再生 / 歯周靭帯 / 歯根膜細胞 / シグナル伝達 / ヒト / 伸展力 |
|---|
| Research Abstract |
We identified Wnt among 216 genes which were up-regulated in stretch loaded human periodontal ligament (PDL) cells, and we hypothesized that Wnt could regenerate PDL. Exogenous Wnt5a induced PDL differentiation related genes expression and stimulated migration of PDL cells. Furthermore, we clarified that Wnts bind to Nidogen-1, which is an extracellular matrix protein, using Far-western analysis and Mass analysis. Phase separation assay showed that Wnts binding Nidogen-1 wereseparated into hydorophilic phase. In addition, Nidogen-1 induced Wnt3a dependent intracellular signaling.
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