Development of novel periodontal tissue therapy targeting for regeneration of periodontal ligament.

• Project/Area Number: 22592123
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Osaka University (2011-2012); Kyushu University (2010)
• Principal Investigator: FUJII Shinsuke 大阪大学, 医学系研究科, 招へい研究員 (60452786)
• Co-Investigator(Kenkyū-buntansha): AKAMINE Akifumi 九州大学, 歯学研究院, 教授 (00117053) ; MAEDA Hidefumi 九州大学, 大学病院, 講師 (10284514) ; WADA Naohisa 九州大学, 大学病院, 助教 (60380466) ; TOMOKIYO Atsushi 九州大学, 大学病院, 助教 (20507777)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 歯根膜 / Wnt / 細胞外基質 / 再生 / 歯周靭帯 / 歯根膜細胞 / シグナル伝達 / ヒト / 伸展力

Research Abstract

We identified Wnt among 216 genes which were up-regulated in stretch loaded human periodontal ligament (PDL) cells, and we hypothesized that Wnt could regenerate PDL. Exogenous Wnt5a induced PDL differentiation related genes expression and stimulated migration of PDL cells. Furthermore, we clarified that Wnts bind to Nidogen-1, which is an extracellular matrix protein, using Far-western analysis and Mass analysis. Phase separation assay showed that Wnts binding Nidogen-1 wereseparated into hydorophilic phase. In addition, Nidogen-1 induced Wnt3a dependent intracellular signaling.

Research Products

• [Journal Article] Exposure to transforming growth factor-β1 after basic fibroblast growth factor promotes the fibroblastic differentiation of human periodontal ligament stem/progenitor cell lines Cell (2013)
  • Author(s): Kono, K., Maeda, H., Fujii, S., Tomokiyo, A., Yamamoto, N.,Wada, N., Monnouchi, S.,Teramatsu, Y., Hamano, S., Koori, K.,Akamine, A.
  • Journal Title: Tissue Res Volume: 352 Pages: 249-263
  • Peer Reviewed
• [Journal Article] Exposure to transforming growth factor-β1 after basic fibroblast growth factor promotes the fibroblastic differentiation of human periodontal ligament stem/progenitor cell lines. (2013)
  • Author(s): Kono K
  • Journal Title: Cell Tissue Res. Volume: in press Issue: 2 Pages: 249-263
  • DOI: 10.1007/s00441-012-1543-0
  • Peer Reviewed
• [Journal Article] Alternation of extracellular matrix remodeling and apoptosis by activation of the aryl hydrocarbon receptor pathway in human periodontal ligament cells (2012)
  • Author(s): Tomokiyo, A., Maeda, H., Fujii, S.,Monnouchi, S., Wada, N., Hori, K., Koori, K., Yamamoto, N., Teramatsu, Y., Akamine, A.
  • Journal Title: J. Cell. Biochem. Volume: 113 Pages: 3093-3103
  • Peer Reviewed
• [Journal Article] Expression and effects of glial cell line-derived neurotrophic factor on periodontal ligament cells (2012)
  • Author(s): Yamamoto, N., Maeda, H., Tomokiyo, A., Fujii, S., Wada, N., Monnouchi, S., Kono, K., Koori, K., Teramatsu, Y., Akamine, A
  • Journal Title: J. Clin. Periodontol. Volume: 39 Pages: 555-564
  • Peer Reviewed
• [Journal Article] A multipotent clonal human periodontal ligament cell line with neural crest cell phenotypes promotes neurocytic differentiation, migration, and survival (2012)
  • Author(s): Tomokiyo, A., Maeda, H., Fujii, S.,Monnouchi, S., Wada, N., Kono, K., Yamamoto, N., Koori, K., Teramatsu, Y., Akamine, A
  • Journal Title: J. Cell. Physiol Volume: 227 Pages: 2040-2050
  • Peer Reviewed
• [Journal Article] Alternation of extracellular matrix remodeling and apoptosis by activation of the aryl hydrocarbon (2012)
  • Author(s): Tomokiyo et al.
  • Journal Title: J Cell Biochem Volume: 113 Issue: 10 Pages: 3093-3103
  • DOI: 10.1002/jcb.24186
  • Peer Reviewed
• [Journal Article] Expression and effects of glial cell line-derived neurotrophic factor on periodontal ligament cells. (2012)
  • Author(s): Yamamoto et al.
  • Journal Title: J Clin Periodontol Volume: 39 Issue: 6 Pages: 556-564
  • DOI: 10.1111/j.1600-051x.2012.01881.x
  • Peer Reviewed
• [Journal Article] A multipotent clonal human periodontal ligament cell line with neural crest cell phenotypes promotes (2012)
  • Author(s): Tomokiyo et al.
  • Journal Title: J Cell Physiol Volume: 227 Issue: 5 Pages: 2040-2050
  • DOI: 10.1002/jcp.22933
  • Peer Reviewed
• [Journal Article] An in vitro evaluation of two resin-based sealers on proliferation and differentiation of human periodontal ligament cells (2011)
  • Author(s): Maeda, H., Tomokiyo, A., Koori, K., Monnouchi, S., Fujii, S., Wada, N., Kono, K., Yamamoto, N., Saito, T., Akamine A
  • Journal Title: Int. Endod. J. Volume: 44 Pages: 425-431
  • Peer Reviewed
• [Journal Article] Transforming growth factor β1 promotes migration of human periodontal ligament cells through heat shock protein 27 phosphorylation. (2011)
  • Author(s): Kwon, SM., Kim, SA., Fujii, S., Maeda, H., Ahn, SG., Yoon, JH.
  • Journal Title: Biol. Pharm. Bull Volume: 34 Pages: 486-489
  • Peer Reviewed
• [Journal Article] he roles of angiotensin II in stretched periodontal ligament cells (2011)
  • Author(s): Monnouchi, S., Maeda, H., Fujii, S., Tomokiyo, A., Kono, K., Akamine, A.
  • Journal Title: J. Dent. Res. Volume: 90 Pages: 181-185
  • Peer Reviewed
• [Journal Article] Transforming Growth Factor .BETA.1 Promotes Migration of Human Periodontal Ligament Cells through Heat Shock Protein 27 Phosphorylation (2011)
  • Author(s): Kwon, et al
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 34 Issue: 4 Pages: 486-489
  • DOI: 10.1248/bpb.34.486
  • NAID: 130000657835
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] An in vitro evaluation of two resin-based sealers on proliferation and differentiation of human periodontal ligament cells. (2011)
  • Author(s): Maeda, et al.
  • Journal Title: Int Endod J. Volume: (未定)
  • Peer Reviewed
• [Journal Article] The roles of angiotensin II in stretched periodontal ligament cells. (2011)
  • Author(s): Monnouchi, et al.
  • Journal Title: J Dent Res. Volume: 90 Pages: 181-185
  • Peer Reviewed
• [Journal Article] Mineral trioxide aggregate induces bone morphogenetic protein-2 expression and calcification in human periodontal ligament cells (2010)
  • Author(s): Maeda, H., Nakano, T., Tomokiyo, A., Fujii, S., Wada, N., Monnouchi, S., Hori, K., Akamine, A.
  • Journal Title: J. Endod. Volume: 36 Pages: 647-652
  • Peer Reviewed
• [Journal Article] The effects of TGF-β1 on the proliferation and differentiation of human periodontal ligament cells and a human periodontal ligament stem/progenitor cell line (2010)
  • Author(s): Fujii, S., Maeda, H., Tomokiyo, A., Monnouchi, S., Hori, K., Wada, N., Akamine, A.
  • Journal Title: Cell Tissue Res Volume: 342 Pages: 233-242
  • NAID: 120005227206
  • Peer Reviewed
• [Journal Article] Effects of TGF-β1 on the proliferation and differentiation of human periodontal ligament cells and a human periodontal ligament stem/progenitor cell line (2010)
  • Author(s): Fujii, et al.
  • Journal Title: Cell and Tissue Research. Volume: 342 Pages: 233-242
  • NAID: 120005227206
  • Peer Reviewed
• [Presentation] 基底膜蛋白質 Nidogen-1 は Wnt3aと結合し Wnt/βカテニン経路の活性化を促進する (2012)
  • Author(s): 藤井慎介
  • Organizer: 第85回日本生化学会大会
  • Year and Date: 2012-12-15
• [Presentation] Exposure to transforming growth factor-beta1 after basic fibroblast growth factor promotes the fibroblastic differentiation of human periodontal ligament stem/progenitor cell lines (2012)
  • Author(s): Kono, et al
  • Organizer: The 7th Slow Aging Strategy and Reproduction Medicine in the Super Aging Society
  • Place of Presentation: 福岡
  • Year and Date: 2012-02-17
• [Presentation] 基底膜蛋白質Nidogen-1はWnt3aと結合しWnt/βカテニン経路の活性化を促進する (2012)
  • Author(s): 藤井慎介
  • Organizer: 日本生化学会
  • Place of Presentation: 福岡
• [Presentation] Formation of epithelial branched tubules by Wnt and EGF in three-dimensional culture (2012)
  • Author(s): Kikuchi A
  • Organizer: 日本生化学会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] Potency of Mechanical Load in Differentiation of Periodontal Ligament Stem Cells (2011)
  • Author(s): Maeda, et al
  • Organizer: 4th Annual World Congress of Regenerative Medicine & Stem Cell 2011
  • Place of Presentation: 北京(中国)(招待講演)
  • Year and Date: 2011-11-12
• [Presentation] ヒト歯根膜細胞への伸展刺激はInterleukin-11の発現を促進する (2011)
  • Author(s): 門野内, ら
  • Organizer: 第134回日本歯科保存学会春季学術大会
  • Place of Presentation: 浦安
  • Year and Date: 2011-06-09
• [Presentation] Contribution of mechanical load to the regeneration of human periodontal ligament tissues. (2010)
  • Author(s): Maeda, et al.
  • Organizer: International Biomedical and Technology & Healthcare Management Summit
  • Place of Presentation: Jiangyin(China)
  • Year and Date: 2010-11-26
• [Presentation] ヒト歯根膜および歯髄細胞の免疫抑制特性にかんする研究 (2010)
  • Author(s): 和田尚久, ら
  • Organizer: 第133回日本歯科保存学会秋期学術大会
  • Place of Presentation: 長良川国際会議場(岐阜)
  • Year and Date: 2010-10-28
• [Presentation] bFGFが未分化なヒト歯根膜細胞株の線維芽細胞様分化に及ぼす影響について (2010)
  • Author(s): 河野清美, ら
  • Organizer: 第133回日本歯科保存学会秋期学術大会
  • Place of Presentation: 長良川国際会議場(岐阜)
  • Year and Date: 2010-10-28
• [Presentation] 未分化なヒト歯根膜細胞株の分化に及ぼすカルシウムの影響について (2010)
  • Author(s): 郡勝明, ら
  • Organizer: 第133回日本歯科保存学会秋期学術大会
  • Place of Presentation: 長良川国際会議場(岐阜)
  • Year and Date: 2010-10-28
• [Presentation] GDNFがヒト歯根膜細胞の走化性に及ぼす影響について (2010)
  • Author(s): 山本直秀
  • Organizer: 第133回日本歯科保存学会秋期学術大会
  • Place of Presentation: 長良川国際会議場(岐阜)
  • Year and Date: 2010-10-28
• [Presentation] スーパーボンドシーラーの特性について-ヒト歯根膜細胞の増殖と分化に与える影響- (2010)
  • Author(s): 前田英史, ら
  • Organizer: 第31回日本歯内療法学会学術大会
  • Place of Presentation: 東商ホール(東京)
  • Year and Date: 2010-07-24
• [Presentation] The roles of angiotensin II in stretched periodontal ligament cells. (2010)
  • Author(s): Monnouchi, et al.
  • Organizer: 88^<th> General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona(Spain)
  • Year and Date: 2010-07-14
• [Book] Differentiation of Periodontal Stem/Progenitor Cells: Roles of TGF-beta1 In: M.A. (2012)
  • Author(s): Maeda H
  • Total Pages: 8
  • Publisher: Springer
• [Book] Potentials of periodontal ligament stem/progenitor cell lines in regeneration studies (2011)
  • Author(s): Maeda, H., Monnouchi, S., Fujii, STomokiyo, A., Wada, N., Akamine, A
  • Publisher: Oral Craniofac Tissue Eng.
• [Book] Promise of periodontal ligament stem cells in regeneration of periodontium. Stem Cell Res (2011)
  • Author(s): Maeda, H., Wada, N., Fujii, S., Tomokiyo, A., Akamine, A.
  • Total Pages: 33
  • Publisher: Stem Cell Res. Ther
• [Book] Periodontal ligament stem cells (2011)
  • Author(s): Maeda, et al
  • Total Pages: 18
  • Publisher: InTech
• [Book] Potentials of periodontal ligament stem/progenitor cell lines in regeneration studies (2011)
  • Author(s): Maeda, et al
  • Total Pages: 11
  • Publisher: Oral Craniofac Tissue Eng
• [Remarks] 大阪大学大学院医学系研究科分子病態生化学
  • URL: http://www.med.osaka-u.ac.jp/pub/molbiobc/index.html
• [Remarks] 大阪大学大学院医学系研究科分子病態生化学
  • URL: http://www.med.osaka-u.ac.jp/pub/molbiobc/index.html
• [Remarks]
  • URL: http://www.kyushu-u.ac.jp/