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Development of the bacteria origin protease activity and the genetic detection method using the electrochemical technique in endodontic treatment

Research Project

Project/Area Number 22592126
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionKyushu Dental College

Principal Investigator

NISHINO Takanobu  九州歯科大学, 歯学部, 歯学部 (80364157)

Co-Investigator(Kenkyū-buntansha) NAGAYOSHI Masato  九州歯科大学, 歯学部, 特別研修員 (30382419)
SATOU Shinobu  九州工業大学, 工学部, 准教授 (80510677)
KITAMURA Chiaki  九州歯科大学, 歯学部, 教授 (50265005)
NISHIHARA Tatsuji  九州歯科大学, 歯学部, 教授 (80192251)
大塚 圭一  九州工業大学, 工学部, 助教 (70448439)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords根管治療 / 細菌由来プロテアーゼ / 電気化学的検出 / 無菌化
Research Abstract

In order to raise the success rate of endodontic treatment, it is necessary to precisely detect the bacteria which exist in a root canal or a periapical lesion. Therefore, a quick, simple, and positive bacteriological detection system at a chair side is strongly desired. In this study, detection of the bacteria by a fluorescence staining agent, and measurement by the electrochemical technique, were examined. Our results suggest possibility of a device to detect bacteria easily and precisely.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (30 results)

All 2013 2012 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (24 results)

  • [Journal Article] 教科書にみる歯内治療の科学的根拠と経験2013

    • Author(s)
      北村知昭,永吉雅人,西野宇信,鷲尾絢子 ,平田-土屋志津,市丸ー末松美希,吉居慎二,西藤法子,廉 クイ勲,中川愛加,中山 皓 平
    • Journal Title

      九州歯科学会雑誌

      Volume: 67(1) Pages: 1-4

    • NAID

      10031174146

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Local regeneration of dentin-pulp complex using controlled release of FGF-2 and naturally derived sponge-like scaffolds.2012

    • Author(s)
      Kitamura C, Nishihara T, Terashita M, Tabata Y, Washio A
    • Journal Title

      International Journal of Dentistry

      Volume: volume2012 Pages: 8-8

    • DOI

      10.1155/2012/190561

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical Detection of Periodontal Disease Using Protease Assay2012

    • Author(s)
      Shinichiro Nagata, Takeshi Ohshima, Keiichi Ohtsuka, Shinobu Sato, Masato Nagayoshi, Chiaki Kitamura, Tatsuji Nishihara, & Shigeori Takenaka
    • Journal Title

      Peptide Science 2011

      Volume: 1 Pages: 407-408

    • NAID

      10030204660

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regeneration approach for dental pulp and periapical tissues with growth factors, biomaterials, and laser irradiation.2011

    • Author(s)
      Chiaki Kitamura, Tatsuji Nishihara, Masamichi Terashita, Yasuhiko Tabata, Eijiro Jimi, Ayako Washio and Shizu Hirata
    • Journal Title

      Polymers

      Volume: 3 Issue: 4 Pages: 1776-1793

    • DOI

      10.3390/polym3041776

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bactericidal Effects of Diode Laser Irradiation on Enterococcus faecalis Using Periapical Lesion Defect Mode2011

    • Author(s)
      Nagayoshi M, Nishihara T, Nakashima K, Iwaki S, Chen KK, Terashita M, Kitamura C.
    • Journal Title

      ISRN Dent

      Volume: 870364 Pages: 6-6

    • DOI

      10.5402/2011/870364

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 歯科保存学の現状と先端科学2010

    • Author(s)
      北村知昭, 西野宇信, 矢野淳也, 永吉雅人, 鷲尾絢子, 平田志津, 吉居慎二, 西藤法子
    • Journal Title

      九州歯科学会雑誌

      Volume: 64 Pages: 66-73

    • NAID

      10026983249

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 3D FEA of fiber post-core construction for endodonticallytreated teeth2012

    • Author(s)
      Nishino T
    • Organizer
      The Fifth Japan-Korea Joint Symposium on Bio-microsensing Technology
    • Place of Presentation
      北九州
    • Year and Date
      2012-10-26
    • Related Report
      2012 Final Research Report
  • [Presentation] フェロセン化ペプチド修飾電極を用いた歯周病関連酵素ジンジパインの活性検出2012

    • Author(s)
      島本隼平
    • Organizer
      日本分析化学会第610会
    • Place of Presentation
      金沢
    • Year and Date
      2012-09-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯内治療における術後疼痛の分析-感染根管治療における術後疼痛の発生頻度に影響を与える因子の解析2012

    • Author(s)
      永吉雅人
    • Organizer
      第33回日本歯内療法学会学術大会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯内治療における術後疼痛の分析 -(1)根管治療中の術後疼痛の発生頻度と根尖孔穿通が術後疼痛出現に与える影響の解析2012

    • Author(s)
      永吉雅人
    • Organizer
      第72回九州歯科学会総会
    • Place of Presentation
      北九州
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯内治療における術後疼痛の分析 ―(1)根管治療中の術後疼痛の発生頻度と根尖孔穿通が術後疼痛出現に与える影響の解析2012

    • Author(s)
      永吉雅人
    • Organizer
      第72回九州歯科学会総会
    • Place of Presentation
      九州歯科大学(北九州)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 歯内治療における術後疼痛の分析―感染根管治療における術後疼痛の発生頻度に影響を与える因子の解析2012

    • Author(s)
      永吉雅人
    • Organizer
      第33回日本歯内療法学会学術大会
    • Place of Presentation
      日経ホール(東京)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ファイバーポスト支台築造システムの光伝達性についての評価2012

    • Author(s)
      西野宇信
    • Organizer
      第72回九州歯科学会総会
    • Place of Presentation
      九州歯科大学(北九州)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Properties of optical fiber as a post for the core construction of non-vital tooth2011

    • Author(s)
      西野宇信
    • Organizer
      The 4th Japan-Korea joint symposium on Bio-microsensing technology
    • Place of Presentation
      Kitakyushu
    • Year and Date
      2011-10-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 感染根管治療時の意図的根尖狭窄部拡大が術後疼痛発生に与える影響と非歯原性疼痛との関連の検討2011

    • Author(s)
      永吉雅人
    • Organizer
      日本歯科保存学会秋季学術大会
    • Place of Presentation
      大阪
    • Related Report
      2012 Final Research Report
  • [Presentation] 感染根管治療時の意図的根尖狭窄部拡大が術後疼痛発生に与える影響と非歯原性疼痛との関連の検討2011

    • Author(s)
      永吉雅人
    • Organizer
      第135回日本歯科保存学会2011年度秋季学術大会
    • Place of Presentation
      大阪
    • Related Report
      2011 Annual Research Report
  • [Presentation] 感染根管治療時の術後疼痛発症頻度および非歯原性疼痛との関連についての検討2011

    • Author(s)
      永吉雅人
    • Organizer
      第16回日本口腔顔面痛学会
    • Place of Presentation
      大阪
    • Related Report
      2011 Annual Research Report
  • [Presentation] 歯内治療における術後疼痛の分析-感染根管治療における根尖狭窄部拡大が術後疼痛の発生頻度に与える影響-2011

    • Author(s)
      永吉雅人
    • Organizer
      第32回日本歯内療法学会学術大会
    • Place of Presentation
      長崎
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光学測色機器クリスタルアイを用いた前歯部天然歯の色調分析2011

    • Author(s)
      西野宇信
    • Organizer
      第134回日本歯科保存学会
    • Place of Presentation
      千葉
    • Related Report
      2011 Annual Research Report
  • [Presentation] 高精度イメージファイバによる根管内観察法の検討2011

    • Author(s)
      北村知昭
    • Organizer
      第71回九州歯科学会総会
    • Place of Presentation
      北九州
    • Related Report
      2011 Annual Research Report
  • [Presentation] クリスタルアイを用いた日本人天然歯の色調についての検討2011

    • Author(s)
      西野宇信
    • Organizer
      第71回九州歯科学会総会
    • Place of Presentation
      北九州
    • Related Report
      2011 Annual Research Report
  • [Presentation] Quantification of Endodontic bacteria using Propidium Monoazide(PMA)2010

    • Author(s)
      NAGAYOSHI Masato
    • Organizer
      with Real-time PCR., Annual Meeting of Japanese Association for Dental Research
    • Place of Presentation
      北九州
    • Related Report
      2012 Final Research Report
  • [Presentation] Propidium monoazide(PMA)を用いたReal-time PCR法による根管内細菌の定量化の試み2010

    • Author(s)
      永吉雅人
    • Organizer
      日本歯科保存学会秋季学術大会
    • Place of Presentation
      岐阜
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] 実習法の違いによる治療術式修得効果への影響 回転式ニッケルチタン製ファイルによる根管形成2010

    • Author(s)
      矢野淳也
    • Organizer
      九州歯科学会
    • Place of Presentation
      北九州
    • Related Report
      2010 Annual Research Report
  • [Presentation] 実習法の違いによる治療術式修得効果への影響 ミラー使用時の窩洞形成2010

    • Author(s)
      西野宇信
    • Organizer
      九州歯科学会
    • Place of Presentation
      北九州
    • Related Report
      2010 Annual Research Report
  • [Presentation] 実習法の違いが治療術式習得へ及ぼす影響 ニッケルチタンファイルによる根管形成2010

    • Author(s)
      矢野淳也
    • Organizer
      日本歯科保存学会春期学術大会
    • Place of Presentation
      熊本
    • Related Report
      2010 Annual Research Report
  • [Presentation] 窩洞形成時のミラー・スキル修得に実習方法の違いが与える影響2010

    • Author(s)
      西野宇信
    • Organizer
      日本歯科保存学会秋季学術大会
    • Place of Presentation
      岐阜
    • Related Report
      2010 Annual Research Report
  • [Presentation] Quantification of Endodontic bacteria using Propidium Monoazide(PMA), with Real-time PCR.2010

    • Author(s)
      NAGAYOSHI Masato
    • Organizer
      Annual Meeting of Japanese Association for Dental Research
    • Place of Presentation
      Kitakyushu
    • Related Report
      2010 Annual Research Report
  • [Presentation] 3D FEA of fiber post-core construction for endodontically treated teeth.

    • Author(s)
      Nishino T
    • Organizer
      The Fifth Japan-Korea Joint Symposium on Bio-microsensing Technology, Kitakyushu, Japan
    • Place of Presentation
      九州工業大学戸畑キャンパス(北九州)
    • Related Report
      2012 Annual Research Report
  • [Presentation] フェロセン化ペプチド修飾電極を用いた歯周病関連酵素ジンジパインの活性検出

    • Author(s)
      島本隼平
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学角間キャンパス(金沢)
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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