The effects of occlusal activity on AChR clustering in mouse masticatory muscles

• Project/Area Number: 22592170
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: Tsurumi University
• Principal Investigator: ISHIKAWA Chieko 鶴見大学, 歯学部, 助教 (70176163)
• Co-Investigator(Kenkyū-buntansha): YAMANE Akira 鶴見大学, 歯学部, 教授 (20166763)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 歯科補綴学一般 / mi/miマウス / 非咬合状態 / アセチルコリン受容体 / クラスター / 共焦点レーザー顕微鏡 / シナプス形成 / クラスター形成 / 三次元構築 / 断片化 / mitf遺伝子 / 小眼球症マウス(mi/mi) / 咬筋 / 生後発達 / ACh受容体クラスター形成 / ブンガロトキシン

Research Abstract

Nicotinic acetylcholine receptor (nAChR) clustering is a key event in the synaptogenesis of the neuromuscular junction (NMJ) for the efficient transmission of neural signals from motor neurons to skeletal muscle. The present study attempted to elucidate the contribution of occlusion to the clustering of nAChR in the NMJ of the masseter, with mi/mi as a model system. The present results indicate abnormal formation of the nAChR cluster in the NMJ of the masseter of mi/mi, suggesting that occlusion is essential for the normal progress of nAChR clustering in the NMJ.

Research Products

• [Journal Article] Role of occlusion in mouse masseter muscle acetylcholine receptor clustering. (2013)
  • Author(s): MORI, M., NARIYAMA, M., ABO, T., HIRAI, S., OGAWA, T., HAMADA, Y., YAMANE, A. and ASADA, Y.
  • Journal Title: J. Dent. Volume: Res. 92(4) Pages: 352-357
  • Peer Reviewed
• [Journal Article] Role of occlusion in mouse masseter muscle acetylcholine receptor clustering (2013)
  • Author(s): MORI, M., NARIYAMA, M., ABO, T., HIRAI, S., OGAWA, T., HAMADA, Y., YAMANE, A. and ASADA, Y.
  • Journal Title: J. Dent. Res. Volume: 92 Issue: 4 Pages: 352-357
  • DOI: 10.1177/0022034513476038
  • Peer Reviewed
• [Journal Article] Age-related changes in rat genioglossus, geniohyoid, and masseter muscles (2013)
  • Author(s): KANEKO, S., IIDA, R., SUGA, T., MORITO, M. and YAMANE, A.
  • Journal Title: Gerodontology Volume: 未定 Issue: 1 Pages: 56-62
  • DOI: 10.1111/ger.12004
  • Peer Reviewed
• [Journal Article] Association between the lack of teeth and the expression of myosins in masticatory muscles of microphthalmic mouse. (2012)
  • Author(s): NARIYAMA, M., KOTA, Y., KANEKO, S., ASADA, Y. and YAMANE, A.
  • Journal Title: Cell Biochem. Func. Volume: 30(1) Pages: 82-88
  • Peer Reviewed
• [Journal Article] The function of platelet derived growth factor in the differentiation of mouse tongue striated muscle. (2012)
  • Author(s): SUZUKI, E., AOYAMA, K., FUKUI, T., NAKAMURA, Y. and YAMANE, A.
  • Journal Title: Orthod. Craniofac. Volume: Res.15(1) Pages: 39-51
  • Peer Reviewed
• [Journal Article] IGF and myostatin pathways are respectively induced during the earlier and the later stages of skeletal muscle hypertrophy induced by clenbuterol, a b2-adrenergic agonist. (2012)
  • Author(s): ABO, T., IIDA, R., KANEKO, S., SUGA, T., YAMADA, H., HAMADA, Y. and YAMANE, A.
  • Journal Title: CellBiochem. Func. Volume: 30(8) Pages: 671-676
  • Peer Reviewed
• [Journal Article] Association between the lack of teeth and the expression of myosins in masticatory muscles of microphthalmic mouse (2012)
  • Author(s): NARIYAMA, M., KOTA, Y., KANEKO, S., ASADA, Y. and YAMANE, A.
  • Journal Title: Cell Biochem. Func. Volume: 30 Issue: 1 Pages: 82-88
  • DOI: 10.1002/cbf.1821
  • Peer Reviewed
• [Journal Article] The function of platelet derived growth factor in the differentiation of mouse tongue striated muscle (2012)
  • Author(s): SUZUKI, E., AOYAMA, K., FUKUI, T., NAKAMURA, Y. and YAMANE, A.
  • Journal Title: Orthod. Craniofac. Res. Volume: 15 Issue: 1 Pages: 39-51
  • DOI: 10.1111/j.1601-6343.2011.01535.x
  • Peer Reviewed
• [Journal Article] IGF and myostatin pathways are respectively induced during the earlier and the later stages of skeletal muscle hypertrophy induced by clenbuterol, a b2-adrenergic agonist (2012)
  • Author(s): ABO, T., IIDA, R., KANEKO, S., SUGA, T., YAMADA, H., HAMADA, Y. and YAMANE, A.
  • Journal Title: Cell Biochem. Func. Volume: 30 Issue: 8 Pages: 671-676
  • DOI: 10.1002/cbf.2848
  • Peer Reviewed
• [Journal Article] Autophagic-lysosomal pathway functions in the masseter and tongue muscles in the klotho mouse, a mouse model for aging. (2011)
  • Author(s): IIDA, R., KANEKO, S., SUGA, T., MORITO, M. and YAMANE, A.
  • Journal Title: Mol. Cell. Biochem. Volume: 89-98
  • Peer Reviewed
• [Journal Article] Bone morphogenetic protein-2 functions as a negative regulator, but not as an inducer for the formations of cartilage and bone in cultured tongue, in the differentiation of myoblasts in mouse embryonic tongue. (2011)
  • Author(s): AOYAMA, K., YAMANE, A., SUGA, T., SUZUKI, E., FUKUI, T. and NAKAMURA, Y.
  • Journal Title: BMC Dev. Biol. Volume: 11:44
  • Peer Reviewed
• [Journal Article] Changes in triacylglycerol-accumulated fiber type, fiber type composition, biogenesis in the mitochondria of the soleus muscle in obese rats. (2011)
  • Author(s): KANEKO, S., IIDA, R., SUGA, T., FUKUI, T., MORITO, M. and YAMANE, A.
  • Journal Title: Anat. Rec. Volume: 294(11) Pages: 1904-1912
  • Peer Reviewed
• [Journal Article] Autophagic-lysosomal pathway functions in the masseter and tongue muscles in the klotho mouse, a mouse model for aging (2011)
  • Author(s): IDA, R., KANEKO, S., SUGA, T., MORITO, M., YAMANE, A.
  • Journal Title: Mol.Cell.Biochem. Volume: 348 Issue: 1-2 Pages: 89-98
  • DOI: 10.1007/s11010-010-0642-z
  • Peer Reviewed
• [Journal Article] Bone morphogenetic protein-2 functions as a negative regulator, but not as an inducer for the formations of cartilage and bone in cultured tongue, in the differentiation of myoblasts in mouse embryonic tongue (2011)
  • Author(s): AOYAMA, K., YAMANE, A., SUGA, T., SUZUKI, E., FUKUI, T., NAKAMURA, Y.
  • Journal Title: BMC Dev.Biol. Volume: 44 Issue: 1 Pages: 11-11
  • DOI: 10.1186/1471-213x-11-44
  • Peer Reviewed
• [Journal Article] Changes in triacylglycerol-accumulated fiber type, fiber type composition, biogenesis in the mitochondria of the soleus muscle in obese rats (2011)
  • Author(s): KANEKO, S., IIDA, R., SUGA, T., FUKUI, T., MORITO, M., YAMANE, A.
  • Journal Title: Anat.Rec. Volume: 294 Issue: 11 Pages: 1904-1912
  • DOI: 10.1002/ar.21472
  • Peer Reviewed
• [Journal Article] Changes in the expression of myosins during postnatal development of masseter muscle in the microphthalmic mouse. (2010)
  • Author(s): KATAYMA, R., YAMANE, A. and FUKUI, T.
  • Journal Title: Open Dent. J.
  • Peer Reviewed
• [Journal Article] TGFb3 is expressed in differentiating muscle of the embryonic mouse tongue. (2010)
  • Author(s): YAMANE, A. FUKUI, T., SUGA, T., IIDA, R. and MORITO, M.
  • Journal Title: Int. J. Dev. Volume: Biol.54(1) Pages: 221-226
  • Peer Reviewed
• [Journal Article] Effect of the classic 1 week glycogen-loading regimen on the fat-loading in the rat and human. (2010)
  • Author(s): SHINOHARA, A., TAKAKURA, J., YAMANE, A. and SUZUKI, M.
  • Journal Title: J. Nutr. Sci. Volume: Vitaminol.56(5) Pages: 299-304
  • NAID: 10026966065
  • Peer Reviewed
• [Journal Article] Effect of third molar absence on development of masticatory muscles in EL mice. (2010)
  • Author(s): NARIYAMA, M., KOTA, Y., TASHIMA, I., TOMOHIRO, T., YAMANE, A. and ASADA, Y.
  • Journal Title: Pediatric Dent. J. Volume: 20(2) Pages: 122-129
  • NAID: 10027485798
  • Peer Reviewed
• [Journal Article] Changes in the lingualmuscles of obese rats induced by high-fat diet. (2010)
  • Author(s): SAITO, T., YAMANE, A., KANEKO, S., OGAWA, T., IKAWA, T., SAITO, K. and SUGISAKI, M.
  • Journal Title: Arch. Oral Biol. Volume: 55(10) Pages: 803-808
  • Peer Reviewed
• [Journal Article] Changes in the expression of myosins during postnatal development of masseter muscle in the microphthalmic mouse (2010)
  • Author(s): KATAYMA, R., YAMANE, A., FUKUI, T.
  • Journal Title: Open Dent.J. Volume: 4 Pages: 1-7
  • Peer Reviewed
• [Journal Article] TGFβ3 is expressed in differentiating muscle of the embryonic mouse tongue (2010)
  • Author(s): YAMANE, A., FUKUI, T., SUGA, T., IIDA, R., MORITO, M.
  • Journal Title: Int.J.Dev.Biol. Volume: 54 Pages: 221-226
  • Peer Reviewed
• [Journal Article] BMP-2 regulates the formation of oral sulcus in mouse tongue by altering the balance between TIMP-1 and MMP-13 (2010)
  • Author(s): FUKUI, T., SUGA, T., IIDA, R., MORITO, M., LUAN, X., DIEKWISCH, T.G.H., NAKAMURA, Y., YAMANE, A.
  • Journal Title: Anat.Rec. Volume: 293 Pages: 1408-1415
  • Peer Reviewed
• [Journal Article] Investigation of Trends and Characteristics in Patients with Obstructive Sleep Apnea. (2010)
  • Author(s): Chieko ISHIKAWA, Yuko SHIGETA, Takumi OGAWA, et al.
  • Journal Title: The Journal of Indian Prosthodontic Society Volume: 10 Pages: 57-63
  • Peer Reviewed
• [Journal Article] Effect of the classic 1 week glycogen-loading regimen on the fat-loading in the rat and human (2010)
  • Author(s): SHINOHARA, A., TAKAKURA, J., YAMANE, A., SUZUKI, M.
  • Journal Title: J.Nutr.Sci.Vitaminol. Volume: 56 Pages: 299-304
  • NAID: 10026966065
  • Peer Reviewed
• [Journal Article] Effect of third molar absence on development of masticatory muscles in EL mice (2010)
  • Author(s): NARIYAMA, M., KOTA, Y., TASHIMA, I., TOMOHIRO, T., YAMANE, A., ASADA, Y.
  • Journal Title: Pediatric Dent.J. Volume: 20 Pages: 122-129
  • NAID: 10027485798
  • Peer Reviewed
• [Journal Article] Age-related changes in rat genioglossus, geniohyoid, and masseter muscles.
  • Author(s): KANEKO, S., IIDA, R., SUGA, T., MORITO, M. and YAMANE, A.
  • Journal Title: Gerodontology Volume: (In press)
  • Peer Reviewed
• [Journal Article] BMP-2 regulates the formation of oral sulcus in mouse tongue by altering the balance between TIMP-1 and MMP-13.
  • Author(s): FUKUI, T., SUGA, T., IIDA, R., MORITO, M., LUAN, X., DIEKWISCH, T.G.H., NAKAMURA, Y. and YAMANE, A.
  • Journal Title: Anat. Volume: 2010 Pages: 1408-1415
  • Peer Reviewed
• [Journal Article] IGF and myostatin pathways are respectively induced during the earlier and the later stages of skeletal muscle hypertrophy induced by clenbuterol, a β2-adrenergic agonist
  • Author(s): ABO, T., IIDA, R., KANEKO, S., SUGA, T., YAMADA, H., HAMADA, Y., YAMANE, A.
  • Journal Title: Cell Biochem.Func. Volume: (In press)
  • Peer Reviewed
• [Journal Article] Changes in the lingual muscles and volume of upper airway in obese rats
  • Author(s): SAITO, T., YAMANE, A., KANEKO, S., OGAWA, T., IKAWA, T., SAITO, K., SUGISAKI, M.
  • Journal Title: Arch.Oral Biol. Volume: (In press)
• [Presentation] 咬合状態の変化がマウス咬筋の神経筋接合部におけるアセチルコリン受容体クラスター形成に及ぼす影響の解明 (2011)
  • Author(s): 森愛美、成山明具美、阿保徳寿、山根明、朝田芳信
  • Organizer: 第26回日本小児歯科学会関東地方会大会
  • Place of Presentation: ホテルニューオータニ幕張、千葉
  • Year and Date: 2011-10-16
• [Presentation] 咬合状態がマウス咬筋の神経筋接合部におけるアセチルコリン受容体クラスター形成に及ぼす影響 (2011)
  • Author(s): 森愛美、成山明具美、阿保徳寿、山根明、朝田芳信
  • Organizer: 第53回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 長良川国際会議場、岐阜
  • Year and Date: 2011-10-01
• [Presentation] 舌、咀噛筋の発生、発達、再生 (2010)
  • Author(s): 山根明
  • Organizer: 第71回鶴見大学歯学会例会
  • Place of Presentation: 鶴見大学会館
  • Year and Date: 2010-06-19