| Project/Area Number |
22592174
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Prosthetic dentistry
|
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| Research Institution | Osaka Dental University |
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Principal Investigator |
|
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| Co-Investigator(Kenkyū-buntansha) |
MATSUNO Tomonori 日本歯科大学, 歯学部, 准教授 (80199827)
TAKEDA Shoji 大阪歯科大学, 歯学部, 教授 (20067185)
|
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Tatsuo 京都大学, 再生医科学研究所, 准教授 (70227908)
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 顎顔面補綴 / iPS 細胞 / 顎骨再生 / iPS細胞 / 骨再生 / 大型動物 / 細胞移植 |
|---|
| Research Abstract |
We reported the generation of canine induced pluripotent stem cells by the transcription factors Oct3/4, Sox2, Klf4, and c-Myc into canine embryonic fibroblasts (CEFs). Canine iPS cells were cultured on MEFs under knockout serum replacement and neural stem cell serum free medium. Then, flow cytometry was performed on neural-like cells derived from canine iPS cells to sort for p75 and HNK1. Isolated neural crest (NC) cells were cultured in neural stem cell serum free medium containing osteoblast differentiation factor. NC cells isolated from neural-like cell were observed for differentiation based on p75 and HNK1 staining. ALP activity of NC cells was increased in the osteoblast-induction for 14 days, thus, NC cells might be differentiated to osteoblast-like cells.
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