In vitro embryotoxicity tests of dental monomers by iPS/ES cells
| Project/Area Number |
22592202
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
IMAI Koichi 大阪歯科大学, 歯学部, 准教授 (90103100)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKEDA Shoji 大阪歯科大学, 歯学部, 教授 (20067185)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 発生毒性 / EST / ES 細胞 / 歯科用モノマー / 3 次元培養 / コラーゲン / iPS 細胞 / 接着材 / ES細胞 / iPS細胞 / ３次元培養 / Bis-GMA / EST法 / 細胞毒性 / 接着性モノマー / 3次元培養 / in vitro |
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| Research Abstract |
To evaluate embryotoxicity screening tests for various dental monomers, we improved the protocol of the embryonic stem cell test (EST), which is an in vitro embryotoxicity test. We performed the three-dimensional culture technique, by which cells can come into direct contact with insoluble monomers without using solvents. Glass fibers could be used as scaffolds for three-dimensional cultivation, as well as collagens. Furthermore, as the results of investigating the embryotoxicity of 12 types of commercially available dental adhesive, all were within the level of "non-embryotoxicity".
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Report
(4 results)
Research Products
(51 results)
