Bone regeneration around dental implants with artificial bone hybridized with iPS cells.

• Project/Area Number: 22592228
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Showa University
• Principal Investigator: SHIROTA Tatsuo 昭和大学, 歯学部, 准教授 (60235760)
• Co-Investigator(Kenkyū-buntansha): TAKAMI Masamichi 昭和大学, 歯学部, 講師 (80307058) ; SATO Hana 昭和大学, 歯学部, 普通研究生 (50551222)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 骨再生 / 骨芽細胞 / iPS細胞 / 幹細胞 / 骨の再生医療 / 脂肪幹細胞 / 分化誘導 / 人工骨 / 生体材料 / 顎骨再建

Research Abstract

To establish an effective method of the bone regeneration, we developed new artificial bone hybridized with bone marrow mesenchymal stem cells
(1). Then
(2) weexamined the possibility of the osteogenic and chondrogenic induction of adipose-derived stem cell. And
(3), for the purpose of establishing a generic technology of the bone regeneration using the iPS cell
I examined a culture condition suitable for the differentiation instruction to the osteoblasts of induced pluripotent stem cells, and confirmed the differentiation instruction to osteoblaststic cells.

Research Products

• [Journal Article] A combination of chemical and mechanical stimuli enhances not only osteo- but also chondro-differentiation in adipose-derived stem cells (2012)
  • Author(s): Seika Banka、Yoshiki Mukudai、Yasuto Yoshihama、Tatsuo Shirota、Seiji Kondo、Seiji Shintani
  • Journal Title: J Oral Biosciences Volume: Vol. 54 Pages: 188-195
  • URL: http://dx.doi.org/10.1016/j.job.2012.10.0002
  • Peer Reviewed
• [Journal Article] Honeycomb form β-tricalcium phosphate induces osteogenesis by geometrical property with BMSC. (2012)
  • Author(s): Yagami K, Yanagisawa S, Kuboki Y
  • Journal Title: Biomed Mater Eng. Volume: 21 Issue: 5-6 Pages: 291-306
  • DOI: 10.3233/bme-2012-0677
  • Peer Reviewed
• [Journal Article] A combination of chemical and mechanical stimuli enhances not only osteo- but also chondro-differentiation in adipose-derived stem cells (2012)
  • Author(s): Banka
  • Journal Title: J Oral Biosciences Volume: 54 Issue: 4 Pages: 188-195
  • DOI: 10.1016/j.job.2012.10.002
  • Peer Reviewed
• [Presentation] 脂肪幹細胞(ADSC)から骨形成細胞への分化に対する化学的,機械的刺激の影響 (2011)
  • Author(s): 番家政香,椋代義樹,吉濱泰斗,代田達夫,近藤誠二,新谷 悟
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2011-07-29
• [Presentation] Active hexose correlated compound は炎症性サイトカインによるNO産生を抑制し,間葉系幹細胞の骨芽細胞への初期分化を維持する (2011)
  • Author(s): 八上公利,西澤幹雄,代田達夫,柳沢 茂
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2011-07-29
• [Presentation] Active hexose correlated compound は炎症性サイトカインによるNO産生を抑制し,間葉系幹細胞の骨芽細胞への初期分化を維持する (2011)
  • Author(s): 八上公利,西澤幹雄,代田達夫,定岡 直,柳沢 茂,中村浩志
  • Organizer: 第65回日本口腔科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-04-11