| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
GRIM-19 (gene associated with retinoid-IFN-induced mortality 19) codes for a 16 kDa protein which was originally isolated as a growth suppressive gene product in the interferon-beta (IFN-ss)- and retinoic acid (RA)-induced cell death pathway. GRIM-19 has been shown to suppress tumor cell growth and induce apoptosis by interacting with an oncogenic transcription factor STAT3 and thereby inhibiting the STAT3-regulated gene expression. The present study was undertaken to clarify the expression and functional role of GRIM-19 in oral squamous cell carcinoma (OSCC) cells and the following results were obtained.
1) When expression of GRIM-19 was assessed in OSCC cell lines by Western blotting, co-stimulation with IFN-ss and RA induced a prominent GRIM-19 protein expression in Ca9-22 cells. However, an only marginal expression of GRIM-19 was observed in cell lysate from HSC-2 cells stimulated with IFN-ss and RA
2). Although a constitutive GRIM-19 mRNA expression was detected in both HSC-2 and Ca9-22 cells, IFN-ss and RA had no inductive effect on the GRIM-19 mRNA expression in both cells.
3). IFN-ss induced expression of CXCL11 and TRAIL mRNAs in both cells, suggesting that IFN-ss signaling pathway are intact in these cells. Taken together these results suggested that the expression of GRIM-19 in OSCC cells is regulated at translational or post-translational level but not transcriptional level.
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