• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of regulatory mechanisms of expression of apoptosis inducing factor GRIM-19

Research Project

Project/Area Number 22592244
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionMeikai University

Principal Investigator

MORI Kazumasa  明海大学, 歯学部, 講師 (80372902)

Co-Investigator(Kenkyū-buntansha) OHMORI Yoshihiro  明海大学, 歯学部, 教授 (50194311)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords口腔扁平上皮癌 / アポトーシス / GRIM-19 / STAT3 / IFN / GRIM19 / 扁平上皮癌
Research Abstract

GRIM-19 (gene associated with retinoid-IFN-induced mortality 19) codes for a 16 kDa protein which was originally isolated as a growth suppressive gene product in the interferon-beta (IFN-ss)- and retinoic acid (RA)-induced cell death pathway. GRIM-19 has been shown to suppress tumor cell growth and induce apoptosis by interacting with an oncogenic transcription factor STAT3 and thereby inhibiting the STAT3-regulated gene expression. The present study was undertaken to clarify the expression and functional role of GRIM-19 in oral squamous cell carcinoma (OSCC) cells and the following results were obtained.
1) When expression of GRIM-19 was assessed in OSCC cell lines by Western blotting, co-stimulation with IFN-ss and RA induced a prominent GRIM-19 protein expression in Ca9-22 cells. However, an only marginal expression of GRIM-19 was observed in cell lysate from HSC-2 cells stimulated with IFN-ss and RA
2). Although a constitutive GRIM-19 mRNA expression was detected in both HSC-2 and Ca9-22 cells, IFN-ss and RA had no inductive effect on the GRIM-19 mRNA expression in both cells.
3). IFN-ss induced expression of CXCL11 and TRAIL mRNAs in both cells, suggesting that IFN-ss signaling pathway are intact in these cells. Taken together these results suggested that the expression of GRIM-19 in OSCC cells is regulated at translational or post-translational level but not transcriptional level.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (3 results)

All 2011

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Infiltration of M2 tumor-associated macrophages in oral squamous cell carcinoma correlates with tumor malignancy2011

    • Author(s)
      K. Mori, M. Hiroi, J. Shimada and Y. Ohmori
    • Journal Title

      Cancers

      Volume: 3 Issue: 4 Pages: 3726-3739

    • DOI

      10.3390/cancers3043726

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 口腔領域の腫瘍および上皮性異形成におけるM2 マクロファージの発現動態2011

    • Author(s)
      森 一 将
    • Organizer
      第56回日本口腔外科学会総会・学術大会
    • Place of Presentation
      大阪市
    • Year and Date
      2011-11-22
    • Related Report
      2012 Final Research Report
  • [Presentation] 口腔領域の腫瘍および上皮性異形成におけるM2マクロファージの発現動態2011

    • Author(s)
      森一将
    • Organizer
      第56回日本口腔外科学会総会・学術大会
    • Place of Presentation
      大阪市
    • Year and Date
      2011-11-22
    • Related Report
      2011 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi