| Project/Area Number |
22592264
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
|
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| Research Institution | Nihon University |
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Principal Investigator |
OI Yoshiyuki 日本大学, 歯学部, 教授 (60271342)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Masayuki 日本大学, 歯学部, 准教授 (00300830)
SAITOH Toshiyuki 日本大学, 歯学部, 助教 (50195997)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 歯科麻酔学 / プロポフォール / 大脳皮質 / 抑制性シナプス後電流 / propofol / pain / insular cortex / GABA(A) receptor / IPSC / whole-cell patch-clamp / GABA / patch clamp |
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| Research Abstract |
To examine whether propofol effects on unitary inhibitory postsynaptic currents (uIPSCs) are differentially regulated depending on their pre- and postsynaptic cell types, we performed multiple whole-cell patch-clamp recording from layer V GABAergic interneurons and pyramidal (Pyr) cells of rat insular cortex (IC). Comparing the presynaptic FS and presynaptic non-FS connections, the presynaptic FS connections showed larger enhancement of uIPSC charge transfer by propofol. Among the presynaptic FS connections, FS→Pyr connections showed larger enhancement of uIPSC charge transfer thanFS→interneurons, indicating that the primary target of propofol to GABAergic synaptic transmission is FS→Pyr connections.
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