Pre- and post-synaptic cell type dependent propofol effects on GABAergic synaptic transmission in rat insular cortex.
| Project/Area Number |
22592264
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
OI Yoshiyuki 日本大学, 歯学部, 教授 (60271342)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Masayuki 日本大学, 歯学部, 准教授 (00300830)
SAITOH Toshiyuki 日本大学, 歯学部, 助教 (50195997)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 歯科麻酔学 / プロポフォール / 大脳皮質 / 抑制性シナプス後電流 / propofol / pain / insular cortex / GABA(A) receptor / IPSC / whole-cell patch-clamp / GABA / patch clamp |
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| Research Abstract |
To examine whether propofol effects on unitary inhibitory postsynaptic currents (uIPSCs) are differentially regulated depending on their pre- and postsynaptic cell types, we performed multiple whole-cell patch-clamp recording from layer V GABAergic interneurons and pyramidal (Pyr) cells of rat insular cortex (IC). Comparing the presynaptic FS and presynaptic non-FS connections, the presynaptic FS connections showed larger enhancement of uIPSC charge transfer by propofol. Among the presynaptic FS connections, FS→Pyr connections showed larger enhancement of uIPSC charge transfer thanFS→interneurons, indicating that the primary target of propofol to GABAergic synaptic transmission is FS→Pyr connections.
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Report
(4 results)
Research Products
(70 results)
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[Journal Article] Involvement of AMPA receptorGluR2 and GluR3 trafficking in trigeminal spinal subnucleus caudalis and C1/C2 neurons in acute-facial inflammatory pain.2012
Author(s)
Miyamoto M, Tsuboi Y, Honda K, Kobayashi M, Takamiya K, Huganir RL, Kondo M, Shinoda M, Sessle BJ, Katagiri A, Kita D, Suzuki I, Oi Y, Iwata K
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Journal Title
Related Report
Peer Reviewed
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