| Project/Area Number |
22592294
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Tokyo Medical and Dental University (2011-2012)
Yokohama City University (2010) |
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Principal Investigator |
YONEMITSU Ikuo 東京医科歯科大学, 医歯学総合研究科, 助教 (00431940)
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| Co-Investigator(Kenkyū-buntansha) |
YABUSHITA Tadachika 東京医科歯科大学, 医歯学総合研究科, 非常勤講師 (40420260)
TAKEI Maki 東京医科歯科大学, 医歯学総合研究科, 非常勤講師 (50547445)
|
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| Research Collaborator |
HATTORI Ikuko , 医員
KURODA Yukiko , 大学院生
MIYAZAKI Mutsumi , 大学院生
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 顎関節 / asporin / OA / アスポリン / 負荷頻度 / 下顎頭軟骨 |
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| Research Abstract |
In the experimental group, unilateral masseter muscles of 5-week-old rats were resected to represent the animal model of deviated mandible. Two and four weeks after muscle resection, their condyles were observed to detect whether OA-like change occurs. Micro-CTwas used for three-dimensional morphological analysis. In the experimental group, the cancellous bone volume and bone density of the condyles were decreased in the resected side. For histological examination, the specimens were embedded in paraffin and5-μm-thick serial frontal sections of the TMJ were stained with toluidine blue. As a result, the morphological changes of cartilage layers were similar to the past mandibular functional lateral shift model. Furthermore, immunohistochemical staining of asporinwhich are OA-related protein and of TGF-b1 which participates in cartilage repair were performed. We revealed that the expression of asporin in the temporomandibular joint cartilage in the OA-like animal model
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