The examination of the expression of chemokines and RANKL in root resorption during orthodontic tooth movement

• Project/Area Number: 22592297
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Nihon University
• Principal Investigator: YAMAGUCHI Masaru 日本大学, 松戸歯学部, 准教授 (60333100)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯科矯正 / 歯根吸収 / ケモカイン / RANKL / 歯科矯正学 / IL-8 / MCP-1 / excessive orthodontic force / root resorotion / odontoclastogenesis / root resorption

Research Abstract

The immunoreactivity for CINC-1/CXCR2 , MCP-1 and RANKL were detected in odontoclasts and PDL fibroblasts by the orthodontic force of 50 g on day 7. CF increased the production of IL-8, MCP-1 and RANKL from hPDL cells in a magnitude-dependent manner. Moreover, CINC-1,MCP-1 and RANKL stimulated osteoclastogenesis from rat osteoclast precursor cells.Conclusion: IL-8 (CINC-1), MCP-1 and RANKL may facilitate the process of root resorptiondue to excessive orthodontic force.

Research Products

• [Journal Article] IL-17/Th17 cells in an atopic dermatitis mouse model aggravate orthodontic root resorption in dental pulp. (2013)
  • Author(s): Shimizu M, Yamaguchi M, Utsunomiya M, Yamamoto H, Kasai K.
  • Journal Title: Eur J Oral Sci Volume: 121(2) Issue: 2 Pages: 101-10
  • DOI: 10.1111/eos.12024
  • Peer Reviewed
• [Journal Article] Morphological and Histological Changes in the Mandibular Condyle after Plate Removal in Experimental Models of Short- and Long-term Mandibular Lateral Displacement. (2013)
  • Author(s): Ando J, Yamaguchi M, Fujita S, KawamuraA, Utsunomiya T, Kasai K.
  • Journal Title: J Hard Tissue Biol Volume: 22 Pages: 201-210
  • NAID: 130004480580
• [Journal Article] Effect of compression force on apoptosis in human periodontal ligament cells. (2013)
  • Author(s): Funakoshi M, Yamaguchi M, Asano M,Kasai K.
  • Journal Title: J Hard Tissue Biol Volume: 22 Pages: 41-50
  • NAID: 130004848409
• [Journal Article] Immunohistochemical localization of T-helper 17 cells, IL-17, and RANKL during root resorption induced by excessive orthodontic force in a mouse model of T cell-mediated autoimmune disease (2013)
  • Author(s): Yamaguchi M, Yamada M, Asano M, Fujita S, Kasai K.
  • Journal Title: Int J Oral-Med Sci, Volume: 11(4) Pages: 249-260
  • NAID: 130003375182
  • Peer Reviewed
• [Journal Article] Effect of compression force on apoptosis in human periodontal ligament cells (2013)
  • Author(s): Funakoshi M, Yamaguchi M, Asano M, Kasai K.
  • Journal Title: J Hard Tissue Biol Volume: 22(1) Pages: 41-50
  • NAID: 130004848409
  • Peer Reviewed
• [Journal Article] Th17-cells in atopic dermatitis stimulate orthodontic root resorption. (2012)
  • Author(s): Yamada K, Yamaguchi M, Asano M, FujitaS, Kobayashi R, Kasai K.
  • Journal Title: Oral Dis Volume: 19 Issue: 7 Pages: 683-693
  • DOI: 10.1111/odi.12053
  • Year and Date: 2012-12-25
• [Journal Article] Immunohistochemical localization of T-helper 17 cells, IL-17, and RANKL during root resorption induced by excessive orthodontic force in a mouse model of T cell-mediated autoimmune disease. (2012)
  • Author(s): Yamaguchi M, Yamada M, Asano M, Fujita S, Kasai K.
  • Journal Title: Int J Oral-Med Sci Volume: 11 Pages: 249-260
  • NAID: 130003375182
• [Journal Article] Localization of TNF-α andmacrophages in the periodontalligament during orthodontic tooth movement. (2012)
  • Author(s): Funakoshi M, Yamaguchi M, Fujita S,Kasai K.
  • Journal Title: Int J Oral-Med Sci Volume: 11 Pages: 182-181
• [Journal Article] Orthodontic root resorption was associated with the secretion of IL-6 and IL-8 stimulated by IL-17 in dental pulp cells. (2012)
  • Author(s): Shimizu M, Yamaguchi M, Asano M, FujitaS, Utsunomiya T, Yamamoto H, Kasai K.
  • Journal Title: Int J Oral-Med Sci Volume: 11 Pages: 172-189
• [Journal Article] T-helper 17 cells mediate the osteo/odontoclastogenesis induced by excessive orthodontic forces. (2012)
  • Author(s): Hayashi N, Yamaguchi M, Nakajima R,Utsunomiya T, Yamamoto H, Kasai K.
  • Journal Title: Oral Dis Volume: 18(4) Pages: 375-388
• [Journal Article] Mechanoreceptors, nociceptors and orthodontic tooth movement. (2012)
  • Author(s): Yamaguchi M, Nakajima R, Kasai K.
  • Journal Title: Semin Orthod. Volume: 18(4) Issue: 4 Pages: 249-256
  • DOI: 10.1053/j.sodo.2012.06.003
  • Peer Reviewed
• [Journal Article] T-helper 17 cells mediate the osteo/odontoclastogenesis induced by excessive orthodontic forces. (2012)
  • Author(s): Hayashi N, Yamaguchi M, Nakajima R, Utsunomiya T, Yamamoto H, Kasai K.
  • Journal Title: Oral Dis. Volume: 18(4) Issue: 4 Pages: 375-388
  • DOI: 10.1111/j.1601-0825.2011.01886.x
  • Peer Reviewed
• [Journal Article] Orthodontic root resorption was associated with the secretion of IL-6 and IL-8 stimulated by IL-17 in dental pulp cells. (2012)
  • Author(s): Shimizu M, Yamaguchi M, Asano M, Fujita S, Utsunomiya T, Yamamoto H, Kasai K.
  • Journal Title: Int J Oral-Med Sci, Volume: 11(3) Pages: 172-181
  • Peer Reviewed
• [Journal Article] Localization of TNF-α and macrophages in the periodontal ligament during orthodontic tooth movement. (2012)
  • Author(s): Funakoshi M, Yamaguchi M, Fujita S, Kasai K.
  • Journal Title: Int J Oral-Med Sci Volume: 11(3) Pages: 182-189
  • NAID: 130003375171
  • Peer Reviewed
• [Journal Article] IL-6 and IL-17 in gingival crevicular fluid during orthodontic root resorption (2012)
  • Author(s): Yamaguchi Y, Nariyasu T, Hayashi N, Nakajima R, Fujita S, Yamaguchi M, Kasai K
  • Journal Title: International Journal of Oral-Medical Sciences Volume: 10 Pages: 247-254
  • NAID: 130002051659
  • Peer Reviewed
• [Journal Article] IL-8 and MCP-1 induced by excessive orthodontic force mediates odontoclastogenesis in periodontal tissues. (2011)
  • Author(s): Asano M, Yamaguchi M, Nakajima R,Fujita S, Utsunomiya T, Yamamoto H, Kasai K.
  • Journal Title: Oral Dis Volume: 17(5) Pages: 489-498
• [Journal Article] Expressions of RANKL/RANK and M-CSF/c-fms in root resorption by excessive orthodontic force. (2011)
  • Author(s): Nakano Y, Yamaguchi M, Fujita S, AsanoM, Saito K, Kasai K.
  • Journal Title: Eur J Orthod. Volume: 33(4) Pages: 335-343
• [Journal Article] IL-8 and MCP-1 induced by excessive orthodontic force mediates odontoclastogenesis in periodontal tissues (2011)
  • Author(s): Asano M, Yamaguchi M, Nakajima R, Fujita S, Utsunomiya T, Yamamoto H, Kasai K
  • Journal Title: Oral Diseases Volume: 17 Issue: 5 Pages: 489-498
  • DOI: 10.1111/j.1601-0825.2010.01780.x
  • Peer Reviewed
• [Journal Article] Effects of Relaxin on Relapse After Experimental Tooth Movement in Rats (2011)
  • Author(s): Hirate Y, Yamaguchi M, Utsunomiya T, Yamamoto H, Kasai K
  • Journal Title: International Journal of Oral-Medical Sciences Volume: 10 Pages: 200-210
  • NAID: 130002051651
  • Peer Reviewed
• [Journal Article] Effects of HSP70 on the compression force-induced TNF-α and RANKL expression in human periodontal ligament cells. (2010)
  • Author(s): Mitsuhashi M, Yamaguchi M, Kojima T, Nakajima R, Kasai K.
  • Journal Title: Inflammation Research Volume: 60 Pages: 187-194
  • Peer Reviewed
• [Presentation] ヒト歯髄組織中のIL-17が炎症性サイトカイン産生および歯根吸収に及ぼす影響. (2012)
  • Author(s): 清水真美,山口大,浅野正貴,葛西一貴.
  • Organizer: 第71回日本矯正歯科学会大会
  • Place of Presentation: 盛岡.
  • Year and Date: 2012-09-27
• [Presentation] アレルギーは矯正学的歯の移動時に生じる歯根吸収を増悪する. (2012)
  • Author(s): 山田邦彦,山口大,小林良喜,浅野正貴,安藤淳,宇都宮忠彦,山本正文,山本浩嗣,葛西一貴.
  • Organizer: 第71回日本矯正歯科学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2012-09-27
• [Presentation] メカニカルストレスがヒト歯根膜線維芽細胞の細胞死に与える影響. (2012)
  • Author(s): 船越麻理,山口大,小林良喜,浅野正貴,葛西一貴.
  • Organizer: 第71回日本矯正歯科学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2012-09-27
• [Presentation] TNF-αが矯正学的歯の移動時に生じる歯根吸収に及ぼす影響. (2012)
  • Author(s): 吉野智一,山口大,菊田純,葛西一貴.
  • Organizer: 第71回日本矯正歯科学会大会
  • Place of Presentation: 盛岡.
  • Year and Date: 2012-09-27
• [Presentation] 次世代歯科矯正学の研究シーズ戦略-歯根吸収発現予測診断と後戻り抑制薬-. (2012)
  • Author(s): 山口大.
  • Organizer: BIOtech2012第11回バイオテクノロジー展/技術会議
  • Place of Presentation: 東京
  • Year and Date: 2012-04-25
• [Presentation] アレルギーは矯正学的歯の移動時に生じる歯根吸収を増悪する (2012)
  • Author(s): 山田 邦彦、山口 大、小林 良喜、浅野 正貴、安藤 淳、宇都宮 忠彦、山本 正文、山本 浩嗣、葛西 一貴
  • Organizer: 第71回 日本矯正歯科学会大会
  • Place of Presentation: 盛岡市アイスアリーナ, 岩手
• [Presentation] ヒト歯髄組織中のIL-17が炎症性サイトカイン産生および歯根吸収に及ぼす影響 (2012)
  • Author(s): 清水 真美、山口 大、浅野 正貴、葛西 一貴
  • Organizer: 第71回 日本矯正歯科学会大会
  • Place of Presentation: 盛岡市アイスアリーナ, 岩手
• [Presentation] 歯根吸収発現予測における歯肉溝滲出液の有用性 (2011)
  • Author(s): 山口大
  • Organizer: 第70回日本矯正歯科学会大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-19
• [Presentation] K.HSP70 inhibits the compressionforce-induced TNF-α and RANKL expression. (2011)
  • Author(s): Yamaguchi M, Mituhashi M, kasai
  • Organizer: 10th World of congress on inflammation
  • Place of Presentation: paris, France
• [Presentation] Effects of IL-17 on the expression ofRANKL from human dental pulp cells. (2011)
  • Author(s): Shimizu M, Yamaguchi M, kasai K.
  • Organizer: 10th World of congress on inflammation
  • Place of Presentation: paris, France
• [Presentation] IL-8/RANKL increasedIL-8/RANKL increased by excessive orthodonticforce in periodontal ligament induces orthodontically-induced inflammatory root resorption. (2011)
  • Author(s): Funakoshi M, Yamaguchi M, Asano M,nakojima R, Fujita S, kasai K.
  • Organizer: 10th World of congress on inflammation
  • Place of Presentation: paris, France
• [Presentation] The participation of interleukin 17 and RANKL in root resorption of the allergic mouce. (2011)
  • Author(s): Yamada K, Yamaguchi M, Fujita S, AsanoM, Ando J, Utsunomiya T, Yamamoto H, kasai K.
  • Organizer: 10th World of congress on inflammation
  • Place of Presentation: paris, France
• [Presentation] IL-17stimulates IL-6 productionand osteoclastogenesis in human periodontal ligament cells. (2011)
  • Author(s): Hayashi N, Yamaguchi M, kasai K.
  • Organizer: 10th World of congress on inflammation
  • Place of Presentation: paris, France
• [Presentation] 矯正学的歯の移動時に生じる歯根吸収におけるケモカイン発現の検討 (2010)
  • Author(s): 浅野正貴、山口大、中嶋亮、藤田祥仁、宇都宮忠彦、山本浩嗣、葛西一貴
  • Organizer: 第52回 日本歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Book] In Principles in Contemporary Orthodontics. (2011)
  • Author(s): Yamaguchi M,他