The elucidation of sharing roles of periodontal ligament cells and alveolar bone osteoblasts in orthodontic-force responsive osteoclast formation.

• Project/Area Number: 22592298
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Nihon University
• Principal Investigator: SHIMIZU Noriyoshi 日本大学, 歯学部, 教授 (40154299)
• Research Collaborator: KOTOEMAYAHARA Kotoe 日本大学, 歯学部, 助教 (60440046)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: メカニカルストレス / 破骨細胞 / RANKL / 歯根膜細胞 / 骨芽細胞 / PGE_2 / ﾒｶﾆｶﾙｽﾄﾚｽ / PGE2 / 矯正力

Research Abstract

Periodontal ligament cells (PDLs) produce PGE_2 during orthodontic tooth movement. PGE_2 is a potent osteoclast-inducing factor that induces RANKL. In this study, we compared the functional difference in osteoclastogenesis between human PDLs (HPDLs) and normal human osteoblasts (HOBs) as a direct effect of PGE_2 exposure.We examined the expression of RANKL with or without PGE_2 in HPDLs and HOBs. Then, RAW264.7 cells were co-cultured on HPDLs or HOBs pretreated with PGE_2. PGE_2 exposure increased significantly RANKL expression in HOBs compared with HPDLs. The number of tartrate-resistant acid phosphatase staining osteoclast-like cells from RAW264.7 cells increased significantly by PGE_2 pretreatment in HOBs and was reduced by small interfering RNA knockdown of RANKL. These results suggest that osteoblasts strongly influence the stimulation of osteoclastogenesis via RANKL, induced byPGE2 in periodontal tissues, compared with PDLs.

Research Products

• [Journal Article] Osteoblastsstimulate osteoclastgenesis via RANKL expression more strongly than periodontal ligament cells do in response to PGE2. (2012)
  • Author(s): Mayahara K, Yamaguchi A, Takenouchi H, Kariya T, Taguchi H, Shimizu N
  • Journal Title: Archives of Oral Biology Volume: 57 Pages: 1377-1384
  • Peer Reviewed
• [Journal Article] Osteoblasts stimulate osteoclastgenesis via RANKL expression more strongly than periodontal ligament cells do in response to PGE2. (2012)
  • Author(s): Mayahara K, et al
  • Journal Title: Archives of Oral Biology Volume: 57 Issue: 10 Pages: 1377-1384
  • DOI: 10.1016/j.archoralbio.2012.07.009
  • Peer Reviewed
• [Presentation] Osteoblasts stimulate osteoclast- genesis via RANKL expression more strongly than periodontal ligament cells do in response to PGE2. (2012)
  • Author(s): Mayahara K et al
  • Organizer: 2012 Annual Session of American Association of Orthodontics
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2012-03-04
• [Presentation] Osteoblasts stimulate osteoclastgenesis via RANKL expression more strongly than periodontal ligament cells do in response to PGE2. (2012)
  • Author(s): Mayahara K, et al
  • Organizer: 第112回アメリカ矯正歯科学会大会
  • Place of Presentation: ハワイ、ホノルルコンベンションセンター、アメリカ合衆国
• [Presentation] PGE2刺激による破骨細胞形成は歯根膜細胞でなく骨芽細胞のRANKL発現による (2011)
  • Author(s): 馬谷原琴枝他
  • Organizer: 第70回日本矯正歯科学会大会
  • Place of Presentation: 愛知県名古屋国際会議場
  • Year and Date: 2011-10-18
• [Presentation] PGE_2刺激による破骨細胞形成は歯根膜細胞でなく骨芽細胞のRANKL発現による (2011)
  • Author(s): 馬谷原琴枝
  • Organizer: 第70回日本矯正歯科学会大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-18