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iPS細胞による舌再生とmiRNAの機能:再生療法への展開

Research Project

Project/Area Number 22592300
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionTsurumi University

Principal Investigator

福井 只美  鶴見大学, 歯学部, 助教 (10267544)

Co-Investigator(Kenkyū-buntansha) 中村 芳樹  鶴見大学, 歯学部, 教授 (10097321)
山根 明  鶴見大学, 歯学部, 教授 (20166763)
Project Period (FY) 2010-04-01 – 2013-03-31
Project Status Declined (Fiscal Year 2012)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsiPS細胞 / 舌 / 再生 / 器官培養法 / アンチセンス / in situ hybridization法 / Real-time PCR法 / miR-1 / マウス / 胚葉体 / マトリゲル / 分化誘導法
Research Abstract

昨年度は舌筋の発生・分化過程におけるmiRNAの機能を明らかにする実験を行った。
胎齢11~15日のマウスより舌を摘出し、RNAを分離・精製し、Real time PCR法を用いて、miR-1、24、133、181の面R蝋の発現量を測定した。胎齢11~15日のマウスより舌を摘出し、4%パラフォルムアルデヒドで固定し、Whole mountのmiRNA in situ hybridization法によりmiR-1の舌における分布を解析した。続いて凍結切片を作成し、miRNA in situ hybridization法を用いてmiR-1の筋組織内での分布を解析した。胎齢12~15日のマウスより舌を摘出し、器官培養を行った。培養液にMorpholinoOligonucleotideAnもisenseを添加した。培養終了後、骨格筋の分化マーカーである齪R-1、myogenin、muscle creatine kinase(MCK)の発現をReal-time PCR法より解析した。
miR-133a、miR-199a3p、miR-199a5pは胎齢11,12,13日に大量に発現し、その後減少した。miR4、miR-24は発生の進行に伴って発現が増加し、胎齢13~15日に大量に発現していた。miR-181は胎齢11~15日で発現量が大きく変化しなかった。Whole mountのmiRNA in situ hybridization法によるとmiR-1は舌全体に分布していることが、凍結切片を用いたmiRNA in situ hybridization法によるとmiR-1は舌筋組織に分布していることが明らかになった。アンチセンスを添加して培養した舌においてはmiR-1の増加は抑制されていたが、MCK、Myogeninの増加は抑制されていなかった。
以上の結果から、miR-1は舌筋細胞の分化に何らかの役割を果たしているが、C2C12培養筋芽細胞などとは異なった役割を果たしていることが示唆された。

Report

(2 results)
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (21 results)

All 2012 2011 2010 Other

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (3 results)

  • [Journal Article] Association between the lack of teeth and the expression of myosins in masticatory muscles of microphthalmic mouse2012

    • Author(s)
      NARIYAMA, M., KOTA, Y., KANEKO, S., ASADA, Y. and YAMANE, A.
    • Journal Title

      Cell Biochem. Func.

      Volume: 30 Issue: 1 Pages: 82-88

    • DOI

      10.1002/cbf.1821

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The function of platelet derived growth factor in the differentiation of mouse tongue striated muscle2012

    • Author(s)
      SUZUKI, E., AOYAMA, K., FUKUI, T., NAKAMURA, Y. and YAMANE, A.
    • Journal Title

      Orthod. Craniofac. Res.

      Volume: 15 Issue: 1 Pages: 39-51

    • DOI

      10.1111/j.1601-6343.2011.01535.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autophagic-lysosomal pathway functions in the masseter and tongue muscles in the klotho mouse, a mouse model for aging2011

    • Author(s)
      IDA, R., KANEKO, S., SUGA, T., MORITO, M., YAMANE, A.
    • Journal Title

      Mol.Cell.Biochem.

      Volume: 348 Issue: 1-2 Pages: 89-98

    • DOI

      10.1007/s11010-010-0642-z

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bone morphogenetic protein-2 functions as a negative regulator, but not as an inducer for the formations of cartilage and bone in cultured tongue, in the differentiation of myoblasts in mouse embryonic tongue2011

    • Author(s)
      AOYAMA, K., YAMANE, A., SUGA, T., SUZUKI, E., FUKUI, T., NAKAMURA, Y.
    • Journal Title

      BMC Dev.Biol.

      Volume: 44 Issue: 1 Pages: 11-11

    • DOI

      10.1186/1471-213x-11-44

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in triacylglycerol-accumulated fiber type, fiber type composition, biogenesis in the mitochondria of the soleus muscle in obese rats2011

    • Author(s)
      KANEKO, S., IIDA, R., SUGA, T., FUKUI, T., MORITO, M., YAMANE, A.
    • Journal Title

      Anat.Rec.

      Volume: 294 Issue: 11 Pages: 1904-1912

    • DOI

      10.1002/ar.21472

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anatomical location of various condylar points for jaw movement analysis in Japanese women2010

    • Author(s)
      TOKIWA H., NAKAZAWA F., OZAKI M., NAKAMURA Y.
    • Journal Title

      J.Oral Rehabil

      Volume: 37 Pages: 235-241

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Elimination of transverse dental compensation is critical for treatment of patients with severe facial asymmetry.2010

    • Author(s)
      SEKIYA T., NAKAMURA Y., et. al
    • Journal Title

      Am.J.Orthod.Dentofac.Orthop.

      Volume: 137 Pages: 552-562

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantity and quality assessment of randomized controlled trials on orthodontics practice in PubMed.2010

    • Author(s)
      SHIMADA T., TAKAYAMA H., NAKAMURA Y.
    • Journal Title

      Angle Orthod.

      Volume: 80 Pages: 713-718

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HSPA1A is upregulated in periodontal ligament at early stage of tooth movement in rats.2010

    • Author(s)
      ARAI C., HANADA N., NAKAMURA Y., et. al
    • Journal Title

      Histochem.Cell Biol.

      Volume: 134 Pages: 337-343

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in the expression of myosins during postnatal development of masseter muscle in the microphthalmic mouse2010

    • Author(s)
      KATAYMA R., YAMANE A., FUKUI T.
    • Journal Title

      Open Dent.J.

      Volume: 4 Pages: 1-7

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TGFβ3 is expressed in differentiating muscle of the embryonic mouse tongue2010

    • Author(s)
      YAMANE A., FUKUI T., SUGA T., IIDA R., MORITO M.
    • Journal Title

      Int.J.Dev.Biol.

      Volume: 54 Pages: 221-226

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] BMP-2 regulates the formation of oral sulcus in mouse tongue by altering the balance between TIMP-1 and MMP-132010

    • Author(s)
      FUKUI T., SUGA T., IIDA R., MORITO M., LUAN X., DIEKWISCH T.G.H., NAKAMURA Y., YAMANE A.
    • Journal Title

      Anat.Rec.

      Volume: 293 Pages: 1408-1415

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Klotho遺伝子突然変異マウス骨格筋におけるインスリン様増殖因子およびその受容体mRNA発現2010

    • Author(s)
      飯田良平、菅武雄、山根明、森戸光彦
    • Journal Title

      鶴見歯学雑誌

      Volume: 36 Pages: 95-102

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of the classic 1 week glycogen-loading regimen on the fat-loading in the rat and human2010

    • Author(s)
      SHINOHARA A., TAKAKURA J., YAMANE A., SUZUKI M.
    • Journal Title

      J.Nutr.Sci.Vitaminol.

      Volume: 56 Pages: 299-304

    • NAID

      10026966065

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of third molar absence on development of masticatory muscles in EL mice2010

    • Author(s)
      NARIYAMA M., KOTA Y., TASHIMA I., TOMOHIRO T., YAMANE A., ASADA Y.
    • Journal Title

      Pediatric Dent.J.

      Volume: 20 Pages: 122-129

    • NAID

      10027485798

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Root resorption after experimental tooth movement using super elastic forces in the rat2010

    • Author(s)
      NODA K., ARAI C., NAKAMURA
    • Journal Title

      Eur.J.Orthod

      Volume: 32 Pages: 681-687

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IGF and myostatin pathways are respectively induced during the earlier and the later stages of skeletal muscle hypertrophy induced by clenbuterol, a β2-adrenergic agonist

    • Author(s)
      ABO, T., IIDA, R., KANEKO, S., SUGA, T., YAMADA, H., HAMADA, Y., YAMANE, A.
    • Journal Title

      Cell Biochem.Func.

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in the lingual muscles and volume of upper airway in obese rats

    • Author(s)
      SAITO T., YAMANE A., KANEKO S., OGAWA T., IKAWA T., SAITO K, SUGISAKI M.
    • Journal Title

      Arch.Oral Biol.

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] アンチセンスによるmiR-1の発現抑制がマウス胎仔の舌筋発生に及ぼす影響2011

    • Author(s)
      幕内俊介、山根明、大久保力廣
    • Organizer
      第74回鶴見大学歯学会例会
    • Place of Presentation
      鶴見大学会館、神奈川
    • Year and Date
      2011-12-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] マウス胎仔の舌発生とmicroRNAの発現変化2011

    • Author(s)
      幕内俊介、山根明、大久保力廣
    • Organizer
      日本補綴歯科学会第120回記念学術大会
    • Place of Presentation
      広島国際会議場、広島
    • Year and Date
      2011-05-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] BMP-2はTIMP-1、TIMP-13の発現を変化させることによってマウス舌側溝形成を調節している2010

    • Author(s)
      福井只美、鈴木栄里佳、青山佳代子、山根明、中村芳樹
    • Organizer
      第69回日本歯科矯正学会大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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