Analysis of Genome Methylation Profile in Relation to Common Mechanisms of Periodontitis and Rhematoid Arthritis Onset

• Project/Area Number: 22592309
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Niigata University
• Principal Investigator: KOBAYASHI Tetsuo 新潟大学, 医歯学総合病院, 准教授 (00215344)
• Co-Investigator(Kenkyū-buntansha): KOMATSU Yasutaka 新潟大学, 医歯学総合病院, 助教 (40422597)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯周炎 / リウマチ / インターロイキン６ / ゲノム / メチル化 / サイトカイン / 遺伝子 / タンパク質 / インターロイキン6

Research Abstract

The aim of the present study is to elucidate the role of methylation status of interleukin-6 (IL-6) gene in regulation of signal expression in relation to susceptibility to periodontitis and rheumatoid arthritis (RA), because of the similarities in the pathologic features of these diseases. The results indicated that patients with periodontitis and RA showed the hypomethylated status of IL-6 gene and increased IL-6 levels than healthy control individuals. These results suggest that IL-6 gene methylation may play a role in the pathogenesis of periodontitis and RA.

Research Products

• [Journal Article] Amino acid profiles in relation to chronic periodontitis and rheumatoid arthritis (2014)
  • Author(s): Kobayashi T, Okada M, Ito S, Kobayashi D, Shinhara A, Muramatsu T, Kobayashi T, Narita I, Nakazono K, Murasawa A, Yoshie H
  • Journal Title: Open Journal of Stomatology Volume: 4(2) Pages: 49-55
  • Peer Reviewed
• [Journal Article] Comparative Analysis of Serum Proteins in Relation to Rheumatoid Arthritis and Chronic Periodontitis (2014)
  • Author(s): Yokoyama T, Kobayashi T, Ito S, Yamagata A, Ishida K, Okada M, Oofusa K, Murasawa A, Yoshie H
  • Journal Title: J Periodontol Volume: 85(1) Pages: 103-112
  • Peer Reviewed
• [Journal Article] Assessment of Interleukin-6 Receptor Inhibition Therapy on Periodontal Condition in Patients with Rheumatoid Arthritis and Chronic Periodontitis (2014)
  • Author(s): Kobayashi T, Okada M, Ito S, Kobayashi D, Ishida K, Kojima A, Narita I, Murasawa A, Yoshie H
  • Journal Title: J Periodontol Volume: 85(1) Pages: 57-67
  • Peer Reviewed
• [Journal Article] Comparative Analysis of Serum Proteins in Relation to Rheumatoid Arthritis and Chronic Periodontitis (2014)
  • Author(s): Tomoko Yokoyama, Tetsuo Kobayashi, et al.
  • Journal Title: Journal of Periodontology Volume: 85 Issue: 1 Pages: 103-112
  • DOI: 10.1902/jop.2013.120741
  • Peer Reviewed
• [Journal Article] Amino acid profiles in relation to chronic periodontitis and rheumatoid arthritis (2014)
  • Author(s): Tetsuo Kobayashi, et al.
  • Journal Title: Open Journal of Stomatology Volume: 4 Issue: 02 Pages: 49-55
  • DOI: 10.4236/ojst.2014.42009
  • Peer Reviewed / Open Access
• [Journal Article] Periodontal Treatment Decreases Levels of Antibodies to Porphyromonas gingivalis and Citrulline in Patients with Rheumatoid Arthritis and Periodontitis (2013)
  • Author(s): Okada M, Kobayashi T, Ito S, Yokoyama T, Abe A, Murasawa A, Yoshie H
  • Journal Title: J Periodontol Volume: 84(12)
  • Peer Reviewed
• [Journal Article] Periodontal Treatment Decreases Levels of Antibodies to Porphyromonas gingivalis and Citrulline in Patients with Rheumatoid Arthritis and Periodontitis (2013)
  • Author(s): Moe Okada, Tetsuo Kobayashi, et al.
  • Journal Title: Journal of Periodontology Volume: 84 Issue: 12
  • DOI: 10.1902/jop.2013.130079
  • Peer Reviewed
• [Journal Article] Assessment of Interleukin-6 Receptor Inhibition Therapy on Periodontal Condition in Patients with Rheumatoid Arthritis and Chronic Periodontitis (2013)
  • Author(s): Kobayashi T, Okada M, Ito S, Kobayashi D, Ishida K, Kojima A, Narita I, Murasawa A, Yoshie H
  • Journal Title: J. Periodontol Volume: (in press) Issue: 1 Pages: 57-67
  • DOI: 10.1902/jop.2013.120696
  • Peer Reviewed
• [Journal Article] Interleukin-6 gene promoter methylation in rheumatoid arthritis and chronic periodontitis (2012)
  • Author(s): Ishida K, Kobayashi T, Ito S, Komatsu Y, Yokoyama T, Okada M, Abe A, Murasawa A, Yoshie H
  • Journal Title: J Periodontol Volume: 83(7) Pages: 917-925
  • Peer Reviewed
• [Journal Article] Interleukin-6 gene promoter methylation in rheumatoid arthritis and chronic periodontitis (2012)
  • Author(s): Ishida K, Kobayashi T, Ito S, Komatsu Y, Yokoyama T, Okada M, Abe A, Murasawa A, Yoshie H
  • Journal Title: J Periodontol Volume: 83(7) Issue: 7 Pages: 917-925
  • DOI: 10.1902/jop.2011.110356
  • Peer Reviewed
• [Journal Article] Antibody responses to periodontopathic bacteria in relation to rheumatoid arthritis in Japanese adults (2011)
  • Author(s): Okada M, Kobayashi T, Ito S, Yokoyama T, Komatsu Y, Abe A, Murasawa A, Yoshie H
  • Journal Title: J Periodontol Volume: 82(10) Pages: 1433-1441
  • Peer Reviewed
• [Journal Article] Antibody responses to periodontopathic bacteria in relation to rheumatoid arthritis in Japanese adults (2011)
  • Author(s): Okada M, Kobayashi T, Ito S, Yokoyama T, Komatsu Y, Abe A, Murasawa A, Yoshie H
  • Journal Title: J.Periodontol Volume: 82(10) Issue: 10 Pages: 1433-1441
  • DOI: 10.1902/jop.2011.110020
  • Peer Reviewed
• [Journal Article] Serum cytokine and periodontal profiles in relation to disease activity of rheumatoid arthritis in Japanese adults (2010)
  • Author(s): Kobayashi T, Murasawa A, Komatsu Y, Yokoyama T, Yamamoto K, Ishida K, Abe A, Yamamoto K, Yoshie H
  • Journal Title: J. Periodontol Volume: 81(5) Pages: 650-657
  • Peer Reviewed
• [Journal Article] Serum cytokine and periodontal profiles in relation to disease activity of rheumatoid arthritis in Japanese adults (2010)
  • Author(s): Tetsuo Kobayashi
  • Journal Title: Journal of Periodontology Volume: VOL.81 Pages: 650-657
  • Peer Reviewed
• [Presentation] 歯周炎罹患組織におけるIL-6遺伝子プロモーター領域のメチル化解析 (2013)
  • Author(s): 石田光平
  • Organizer: 日本歯科保存学会2013年度秋季学術大会(第139回)
  • Place of Presentation: 秋田県総合生活文化会館(秋田県)
  • Year and Date: 2013-10-18
• [Presentation] 慢性歯周炎患者におけるTNF-□遺伝子プロモーター領域のメチル化解析 (2013)
  • Author(s): 小島杏里
  • Organizer: 第56回秋季日本歯周病学会学術大会
  • Place of Presentation: 前橋市民文化会館(群馬県)
  • Year and Date: 2013-09-22
• [Presentation] Periodontal Treatment on Antibodies to Porphyromonas gingivalis and Citrulline levels and Rheumatoid Arthritis Condition (2013)
  • Author(s): Okada M
  • Organizer: The 10th. Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: Nara
  • Year and Date: 2013-09-04
• [Presentation] Tumor Necrosis Factor-Alpha Gene Promoter Methylation in Chronic Periodontitis (2013)
  • Author(s): Kojima A
  • Organizer: The 10th. Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: Nara
  • Year and Date: 2013-09-04
• [Presentation] Effect of Interkeukin-6 Receptor Inhibition Therapy on Periodontal Condition in Patients with Rheumatoid Arthritis and Periodontitis (2013)
  • Author(s): Kobayashi T
  • Organizer: The 10th. Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: Nara
  • Year and Date: 2013-09-03
• [Presentation] Interkeukin-6 Gene Promoter Methylation in Periodontal Tissues (2013)
  • Author(s): Ishida K
  • Organizer: The 10th. Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: Nara
  • Year and Date: 2013-09-03
• [Presentation] 関節リウマチ患者におけるトシリズマブ治療の歯周状態への影響 (2013)
  • Author(s): 小林哲夫
  • Organizer: 日本歯科保存学会2013年度春季学術大会(第138回)
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2013-06-28
• [Presentation] IL-6受容体抑制治療中の関節リウマチ患者の歯周状態 (2013)
  • Author(s): 小林哲夫
  • Organizer: 第56回春季日本歯周病学会学術大会
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2013-05-31
• [Presentation] トシリズマブ(TCZ)療法中の関節リウマチ患者の歯周状態 (2013)
  • Author(s): 小林哲夫
  • Organizer: 第57回日本リウマチ学会総会・学術集会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2013-04-19
• [Presentation] Effect of Interkeukin-6 Receptor Inhibition Therapy on Periodontal Condition in Patients with Rheumatoid Arthritis and Periodontitis (2013)
  • Author(s): Tetsuo Kobayashi
  • Organizer: The 10th. Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: 奈良県新公会堂（奈良県）
• [Presentation] Interkeukin-6 Gene Promoter Methylation in Periodontal Tissues (2013)
  • Author(s): Kohei Ishida
  • Organizer: The 10th. Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: 奈良県新公会堂（奈良県）
• [Presentation] Effects of Periodontal Treatment on Antibodies to Porphyromonas gingivalis and Citrulline levels and Rheumatoid Arthritis Condition (2013)
  • Author(s): Moe Okada
  • Organizer: The 10th. Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: 奈良県新公会堂（奈良県）
• [Presentation] Tumor Necrosis Factor-Alpha Gene Promoter Methylation in Chronic Periodontitis (2013)
  • Author(s): Anri Kojima
  • Organizer: The 10th. Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: 奈良県新公会堂（奈良県）
• [Presentation] IL-6遺伝子プロモーター領域メチル化と慢性歯周炎および関節リウマチ感受性との関連 (2012)
  • Author(s): 石田光平
  • Organizer: 日本歯科保存学会2012年度秋季学術大会(第137回)
  • Place of Presentation: 広島国際会議場(広島県広島市)
  • Year and Date: 2012-11-22
• [Presentation] Improvement of periodontal condition by anti-tumor necrosis factor therapy in rheumatoid arthritis patients (2012)
  • Author(s): Kobayashi T
  • Organizer: EuroPerio 7
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2012-06-07
• [Presentation] Effects of periodontal therapy on the condition of rheumatoid arthritis (2012)
  • Author(s): Okada M
  • Organizer: EuroPerio 7
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2012-06-07
• [Presentation] Interleukin-6 gene promoter methylation in relation to rheumatoid arthritis and periodontitis (2011)
  • Author(s): Ishida K
  • Organizer: The 97th. Annual Meeting of the American Academy of Periodontology
  • Place of Presentation: Miami, Florida, United States of America
  • Year and Date: 2011-11-13
• [Presentation] 関節リウマチおよび歯周炎患者におけるIL-6遺伝子プロモーター領域のメチル化解析 (2011)
  • Author(s): 石田光平
  • Organizer: 第54回秋季日本歯周病学会学術大会
  • Place of Presentation: 海峡メッセ(山口県下関市)
  • Year and Date: 2011-09-24
• [Presentation] DNA methylation status of the interleukin-6 gene promoter in periodontitis (2010)
  • Author(s): Ishida K
  • Organizer: The 96th. Annual Meeting of the American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
  • Place of Presentation: Honolulu, Hawaii, United States of America
  • Year and Date: 2010-11-01
• [Presentation] Serum cytokine and periodontal profiles related to rheumatoid arthritis activity (2010)
  • Author(s): Kobayashi T
  • Organizer: The 96th. Annual Meeting of the American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
  • Place of Presentation: Honolulu, Hawaii, United States of America
  • Year and Date: 2010-11-01
• [Presentation] 歯周炎および関節リウマチ患者におけるIL-6遺伝子プロモーター領域のメチル化解析 (2010)
  • Author(s): 石田光平
  • Organizer: 第53回秋季日本歯周病学会学術大会
  • Place of Presentation: サンポートホール高松(香川県高松市)
  • Year and Date: 2010-09-19
• [Presentation] 粛周炎および関節リウマチ患者におけるIL-6遺伝子プロモーター領域のメチル化解析 (2010)
  • Author(s): 石田光平
  • Organizer: 第53回秋季日本歯周病学会学術大会
  • Place of Presentation: サンポートホール高松(香川県高松市)
  • Year and Date: 2010-09-19
• [Presentation] トシリズマブ（TCZ）療法中の関節リウマチ患者の歯周状態
  • Author(s): 小林哲夫
  • Organizer: 第５７回日本リウマチ学会総会・学術集会
  • Place of Presentation: 京都国際会館（京都府）
• [Presentation] IL-6受容体抑制治療中の関節リウマチ患者の歯周状態
  • Author(s): 小林哲夫
  • Organizer: 第５６回春季日本歯周病学会学術大会
  • Place of Presentation: タワーホール船堀（東京都）
• [Presentation] 関節リウマチ患者におけるトシリズマブ治療の歯周状態への影響
  • Author(s): 小林哲夫
  • Organizer: 日本歯科保存学会2013年度春季学術大会（第138回）
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] 慢性歯周炎患者におけるTNF-alpha遺伝子プロモーター領域のメチル化解析
  • Author(s): 小島杏里
  • Organizer: 第５６回秋季日本歯周病学会学術大会
  • Place of Presentation: 前橋市民文化会館（群馬県）
• [Presentation] 歯周炎罹患組織におけるIL-6遺伝子プロモーター領域のメチル化解析
  • Author(s): 石田光平
  • Organizer: 日本歯科保存学会2013年度秋季学術大会（第139回）
  • Place of Presentation: 秋田県総合生活文化会館（秋田県）
• [Presentation] 抗ＴＮＦ療法による関節リウマチ患者の歯周組織状態の改善
  • Author(s): 小林哲夫
  • Organizer: 第５５回春季日本歯周病学会学術大会
  • Place of Presentation: 札幌コンベンションセンター（札幌市）
• [Presentation] 歯周治療による関節リウマチへの影響
  • Author(s): 岡田 萌
  • Organizer: 第５５回春季日本歯周病学会学術大会
  • Place of Presentation: 札幌コンベンションセンター（札幌市）
• [Presentation] Improvement of periodontal condition by anti-tumor necrosis factor therapy in rheumatoid arthritis patients
  • Author(s): Tetsuo Kobayashi
  • Organizer: EuroPerio 7
  • Place of Presentation: メッセ・ウィーンエキシビションセンター（ウィーン）
• [Presentation] Effects of periodontal therapy on the condition of rheumatoid arthritis
  • Author(s): Moe Okada
  • Organizer: EuroPerio 7
  • Place of Presentation: メッセ・ウィーンエキシビションセンター（ウィーン）
• [Presentation] 歯周炎罹患歯肉におけるIL-6遺伝子プロモーター領域のメチル化解析
  • Author(s): 石田光平
  • Organizer: 日本歯科保存学会2012年度秋季学術大会（第137回）
  • Place of Presentation: 広島国際会議場（広島市）
• [Presentation] 関節リウマチにおける歯周炎関連タンパク質の網羅的解析
  • Author(s): 小林哲夫
  • Organizer: 日本歯科保存学会2011年度春季学術大会（第134回）
  • Place of Presentation: 東京ベイ舞浜ホテルクラブリゾート（千葉県舞浜市）
• [Presentation] 歯周病原細菌に対する血清抗体価と関節リウマチとの関連
  • Author(s): 岡田 萌
  • Organizer: 日本歯科保存学会2011年度春季学術大会（第134回）
  • Place of Presentation: 東京ベイ舞浜ホテルクラブリゾート（千葉県舞浜市）
• [Presentation] 石田光平 関節リウマチおよび歯周炎患者におけるIL-6遺伝子プロモーター領域のメチル化解析
  • Author(s): 石田光平
  • Organizer: 第54回秋季日本歯周病学会学術大会
  • Place of Presentation: 海峡メッセ（山口県下関市）
• [Book] リウマチ病セミナーXXIV (2013)
  • Author(s): 小林哲夫
  • Publisher: 永井書店
• [Remarks] 新潟大学大学院医歯学総合研究科歯周診断・再建学分野
  • URL: http://mds.niigata-u.ac.jp/organization/ols/obs/per/index_j.html
• [Remarks] 新潟大学大学院医歯学総合研究科歯周診断・再建学分野
  • URL: http://mds.niigata-u.ac.jp/organization/ols/obs/per/index_j.html
• [Remarks] 新潟大学大学院医歯学総合研究科歯周診断・再建学分野
  • URL: http://mds.niigata-u.ac.jp/organization/ols/obs/per/index_j.html
• [Remarks] 新潟大学大学院医歯学総合研究科歯周診断・再建学分野
  • URL: http://mds.niigata-u.ac.jp/organization/ols/obs/per/index_j.html
• [Remarks]
  • URL: http://mds.niigata-u.ac.jp/organization/ols/obs/per/index.j.html