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Development of multi-layered stem cell sheet transplant materialfor periodontal regenerative therapy

Research Project

Project/Area Number 22592315
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionNagasaki University

Principal Investigator

ABE Tatsuya  長崎大学, 大学院・医歯薬学総合研究科, 客員研究員 (80271112)

Co-Investigator(Kenkyū-buntansha) HARA Yoshitaka  長崎大学, 大学院・医歯薬学総合研究科, 教授 (60159100)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords移植・再生医療 / 再生医学 / 細胞・組織 / 歯学 / シクナル伝達 / 幹細胞 / 増殖因子 / 接着斑
Research Abstract

In this study, we developed a multi-layered stem cell sheet transplant material with the aim to establish a new method for periodontal regenerative therapy. In the conventional culture medium, the proliferation of the mesenchymal stem cells stopped after forming confluent monolayer. However, the cells continued to grow and formed multi-layer sheets in the presence of TGF-β1 and ascorbic acid. Further addition ofROCK inhibitor Y-27632 to the medium containing TGF-β1 and ascorbic acid prevented the contraction of the cell sheets caused by TGF-β1. The cells in the multi-layered sheetsexpressed the same surface markers with those of the original mesenchymal stem cells and had the potential to differentiate into many cell lineages. These results indicated that this multi-layered stem cell sheet transplant material is useful for periodontal regenerative therapy.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (4 results)

All 2010

All Presentation (4 results)

  • [Presentation] 幹細胞シートの作製2010

    • Author(s)
      中村康平、安部達也、原宜興
    • Organizer
      日本歯周病学会九州5大学・日本臨床歯周病学会九州支部合同研修会
    • Place of Presentation
      福岡県歯科医師会館(福岡市)
    • Year and Date
      2010-11-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 幹細胞シートの作製に関する研究2010

    • Author(s)
      中村康平、安部達也、原宜興
    • Organizer
      第53回秋季日本歯周病学会学術大会
    • Place of Presentation
      かがわ国際会議場(高松市)
    • Year and Date
      2010-09-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] 「幹細胞シートの作製に関する研究」2010

    • Author(s)
      中村康平、安部達也、原宜興
    • Organizer
      第53回秋季日本歯周病学会学術大会
    • Place of Presentation
      高松
    • Related Report
      2012 Final Research Report
  • [Presentation] 「幹細胞シートの作製」2010

    • Author(s)
      中村康平、安部達也、原宜興
    • Organizer
      2010年度日本臨床歯周病学会九州支部・日本歯周病学会九州5大学合同研修会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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