A study on the mechanisms involved in the invasion of host cells by periodontal pathogens and the novel method for its regulation

• Project/Area Number: 22592317
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: SAITO Atsushi 東京歯科大学, 歯学部, 教授 (60266559)
• Co-Investigator(Kenkyū-buntansha): ISHIHARA Kazuyuki 東京歯科大学, 歯学部, 教授 (00212910) ; INAGAKI Satoru 東京歯科大学, 歯学部, 助教 (20385165) ; KOKUBU Eitoyo 東京歯科大学, 歯学部, 助教 (70453785)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: 歯周炎 / 歯周病原細菌 / 侵入 / バイオフィルム / 細胞侵入 / 歯肉上皮細胞 / Porphyromonas gingivalis / Fusobacterium nucleatum / 歯周病 / 歯周病原性菌

Research Abstract

In order to gain insight into the mechanism and significance of host cell invasion by periodontal bacteria in polymicrobial infecti on, we assessedPorphyromonas gingivalis invasion of gingival epithelial cells enhanced by Fusobacterium nucleatum. Confocal scanning laser microscopy and antibiotic protection assay were used to study the role of P. gingivalis invasin (SerB) in this phenomena. The results from the experiment using SerB-mutant strain showed that the P. gingivalis invasin does not play a major role in this phenomenon. It was demonstrated that lipid raft was involved in fusobacterial enhancement of P. gingivalis invasion.

Research Products

• [Journal Article] 細菌における免疫回避機構の解明 (2013)
  • Author(s): 齋藤 淳,加藤哲男,菊池有一郎,国分栄仁,今村健太郎
  • Journal Title: 平成 24 年度東京歯科大学口腔科学研究センターワークショップ, 平成 25 年 3月 1 日,千葉市美浜区. 平成 24 年度東京歯科大学口腔科学研究センターワークショップ プログラムおよび抄録集 Pages: 41-41
  • Peer Reviewed
• [Journal Article] Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum (2012)
  • Author(s): Hosaka, Y., Saito, A., Maeda, R., Fukaya, C., Morikawa, S., Makino, A., Ishihara, K., Nakagawa, T
  • Journal Title: Arch Oral Biol Volume: 57 Issue: 4 Pages: 364-368
  • DOI: 10.1016/j.archoralbio.2011.09.005
  • Peer Reviewed
• [Journal Article] Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species (2012)
  • Author(s): Okuda, T., Kokubu, E., Kawana, T., Saito, A., Okuda, K., Ishihara, K
  • Journal Title: Anaerobe Volume: 18 Issue: 1 Pages: 110-116
  • DOI: 10.1016/j.anaerobe.2011.09.003
  • Peer Reviewed
• [Journal Article] Synergistic effect on biofilm formation between Fusobacterium nucleatum and Capnocytophaga ochracea (2012)
  • Author(s): Okuda, T., Okuda, K., Kokubu, E., Kawana, T., Saito, A., Ishihara, K
  • Journal Title: Anaerobe Volume: 18 Issue: 1 Pages: 157-161
  • DOI: 10.1016/j.anaerobe.2012.01.001
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis entry into gingival epithelial cells modulated by Fusobacterium nucleatum is dependent on lipid rafts (2012)
  • Author(s): Saito A et al.,
  • Journal Title: Microbial Pathogenesis Volume: 53 Issue: 5-6 Pages: 234-242
  • DOI: 10.1016/j.micpath.2012.08.005
  • Peer Reviewed
• [Journal Article] 歯科における最近の抗菌薬の使い方,考え方 5. 歯周病治療と抗菌薬 宿主に潜む歯周病原細菌への対応 (2011)
  • Author(s): 齋藤 淳
  • Journal Title: 歯科医療 Volume: 25 Pages: 37-43
  • URL: http://www.ds-pub.jp/magazinenumber.html
• [Journal Article] 歯科における最近の抗菌薬の使い方,考え方5.歯周病治療と抗菌薬-宿主に潜む歯周病原細菌への対応 (2011)
  • Author(s): 齋藤淳
  • Journal Title: 歯科医療 Volume: 25 Pages: 37-43
• [Presentation] Host cell invaders : the impact of polymicrobial infection by periodontal pathogens on oral and systemic health (2011)
  • Author(s): Saito A
  • Organizer: 11th Yonsei Dental International Symposium
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-12-03
• [Presentation] Periodontopathic biofilm formation of Fusobacterium nucleatum with Prevotella intermedia, Prevotella nigrescens (2011)
  • Author(s): Okuda T, et al
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-06
• [Presentation] Differential ability of periodontopathogens to modulate invasion of gingival epithelial cells by Porphyromonas gingivalis (2010)
  • Author(s): Saito, A., Inagaki, S., Ishihara, K.
  • Organizer: 96^<th> Annual Meeting, American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
  • Place of Presentation: ホノルル,米国
  • Year and Date: 2010-11-01
• [Presentation] Polymicrobial感染における歯周病原性菌Porphyromonas gingivalisの宿主細胞への侵入について (2010)
  • Author(s): 齋藤淳, 稲垣覚, 国分栄仁, 君塚隆太, 石原和幸
  • Organizer: 第93回日本細菌学会関東支部総会
  • Place of Presentation: 東京
  • Year and Date: 2010-10-21
• [Presentation] Porphyromonas gingivalis entry into gingival epithelial cells modulated by Fusobacterium nucleatum is dependent on lipid rafts
  • Author(s): Saito A et al.,
  • Organizer: The 98th Annual Meeting of American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
  • Place of Presentation: Los Angeles, CA, USA
• [Book] Periodontal Disease: Symptoms, Treatment and Prevention (2011)
  • Author(s): Saito A, Inagaki K, Kokubu E, Kimizuka R, Ishihara K
  • Publisher: Nova Science
• [Book] In Molecular detection of human bacterial pathogens (Liu D eds) (2011)
  • Author(s): Ishihara K, Saito A, Inagaki K
  • Publisher: CRC
• [Book] デンタルダイヤモンド増刊号インプラント時代の歯周マネジメント (2011)
  • Author(s): 齋藤淳
  • Publisher: デンタルダイヤモンド
• [Book] Periodontal Disease : Symptoms, Treatment and Prevention (2011)
  • Author(s): Saito, A., Inagaki, K., Kokubu, E., Kimizuka, R., Ishihara, K.
  • Publisher: Nova Science Publishers(in press)