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A study on the mechanisms involved in the invasion of host cells by periodontal pathogens and the novel method for its regulation

Research Project

Project/Area Number 22592317
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionTokyo Dental College

Principal Investigator

SAITO Atsushi  東京歯科大学, 歯学部, 教授 (60266559)

Co-Investigator(Kenkyū-buntansha) ISHIHARA Kazuyuki  東京歯科大学, 歯学部, 教授 (00212910)
INAGAKI Satoru  東京歯科大学, 歯学部, 助教 (20385165)
KOKUBU Eitoyo  東京歯科大学, 歯学部, 助教 (70453785)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywords歯周炎 / 歯周病原細菌 / 侵入 / バイオフィルム / 細胞侵入 / 歯肉上皮細胞 / Porphyromonas gingivalis / Fusobacterium nucleatum / 歯周病 / 歯周病原性菌
Research Abstract

In order to gain insight into the mechanism and significance of host cell invasion by periodontal bacteria in polymicrobial infecti on, we assessedPorphyromonas gingivalis invasion of gingival epithelial cells enhanced by Fusobacterium nucleatum. Confocal scanning laser microscopy and antibiotic protection assay were used to study the role of P. gingivalis invasin (SerB) in this phenomena. The results from the experiment using SerB-mutant strain showed that the P. gingivalis invasin does not play a major role in this phenomenon. It was demonstrated that lipid raft was involved in fusobacterial enhancement of P. gingivalis invasion.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (16 results)

All 2013 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 5 results) Presentation (5 results) Book (4 results)

  • [Journal Article] 細菌における免疫回避機構の解明2013

    • Author(s)
      齋藤 淳,加藤哲男,菊池有一郎,国分栄仁,今村健太郎
    • Journal Title

      平成 24 年度東京歯科大学口腔科学研究センターワークショップ, 平成 25 年 3月 1 日,千葉市美浜区. 平成 24 年度東京歯科大学口腔科学研究センターワークショップ プログラムおよび抄録集

      Pages: 41-41

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum2012

    • Author(s)
      Hosaka, Y., Saito, A., Maeda, R., Fukaya, C., Morikawa, S., Makino, A., Ishihara, K., Nakagawa, T
    • Journal Title

      Arch Oral Biol

      Volume: 57 Issue: 4 Pages: 364-368

    • DOI

      10.1016/j.archoralbio.2011.09.005

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species2012

    • Author(s)
      Okuda, T., Kokubu, E., Kawana, T., Saito, A., Okuda, K., Ishihara, K
    • Journal Title

      Anaerobe

      Volume: 18 Issue: 1 Pages: 110-116

    • DOI

      10.1016/j.anaerobe.2011.09.003

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synergistic effect on biofilm formation between Fusobacterium nucleatum and Capnocytophaga ochracea2012

    • Author(s)
      Okuda, T., Okuda, K., Kokubu, E., Kawana, T., Saito, A., Ishihara, K
    • Journal Title

      Anaerobe

      Volume: 18 Issue: 1 Pages: 157-161

    • DOI

      10.1016/j.anaerobe.2012.01.001

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Porphyromonas gingivalis entry into gingival epithelial cells modulated by Fusobacterium nucleatum is dependent on lipid rafts2012

    • Author(s)
      Saito A et al.,
    • Journal Title

      Microbial Pathogenesis

      Volume: 53 Issue: 5-6 Pages: 234-242

    • DOI

      10.1016/j.micpath.2012.08.005

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 歯科における最近の抗菌薬の使い方,考え方 5. 歯周病治療と抗菌薬 宿主に潜む歯周病原細菌への対応2011

    • Author(s)
      齋藤 淳
    • Journal Title

      歯科医療

      Volume: 25 Pages: 37-43

    • URL

      http://www.ds-pub.jp/magazinenumber.html

    • Related Report
      2012 Final Research Report
  • [Journal Article] 歯科における最近の抗菌薬の使い方,考え方5.歯周病治療と抗菌薬-宿主に潜む歯周病原細菌への対応2011

    • Author(s)
      齋藤淳
    • Journal Title

      歯科医療

      Volume: 25 Pages: 37-43

    • Related Report
      2011 Annual Research Report
  • [Presentation] Host cell invaders : the impact of polymicrobial infection by periodontal pathogens on oral and systemic health2011

    • Author(s)
      Saito A
    • Organizer
      11th Yonsei Dental International Symposium
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2011-12-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] Periodontopathic biofilm formation of Fusobacterium nucleatum with Prevotella intermedia, Prevotella nigrescens2011

    • Author(s)
      Okuda T, et al
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌市
    • Year and Date
      2011-09-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Differential ability of periodontopathogens to modulate invasion of gingival epithelial cells by Porphyromonas gingivalis2010

    • Author(s)
      Saito, A., Inagaki, S., Ishihara, K.
    • Organizer
      96^<th> Annual Meeting, American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
    • Place of Presentation
      ホノルル,米国
    • Year and Date
      2010-11-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Polymicrobial感染における歯周病原性菌Porphyromonas gingivalisの宿主細胞への侵入について2010

    • Author(s)
      齋藤淳, 稲垣覚, 国分栄仁, 君塚隆太, 石原和幸
    • Organizer
      第93回日本細菌学会関東支部総会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Porphyromonas gingivalis entry into gingival epithelial cells modulated by Fusobacterium nucleatum is dependent on lipid rafts

    • Author(s)
      Saito A et al.,
    • Organizer
      The 98th Annual Meeting of American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
    • Place of Presentation
      Los Angeles, CA, USA
    • Related Report
      2012 Annual Research Report
  • [Book] Periodontal Disease: Symptoms, Treatment and Prevention2011

    • Author(s)
      Saito A, Inagaki K, Kokubu E, Kimizuka R, Ishihara K
    • Publisher
      Nova Science
    • Related Report
      2012 Final Research Report
  • [Book] In Molecular detection of human bacterial pathogens (Liu D eds)2011

    • Author(s)
      Ishihara K, Saito A, Inagaki K
    • Publisher
      CRC
    • Related Report
      2012 Final Research Report
  • [Book] デンタルダイヤモンド増刊号インプラント時代の歯周マネジメント2011

    • Author(s)
      齋藤淳
    • Publisher
      デンタルダイヤモンド
    • Related Report
      2011 Annual Research Report
  • [Book] Periodontal Disease : Symptoms, Treatment and Prevention2011

    • Author(s)
      Saito, A., Inagaki, K., Kokubu, E., Kimizuka, R., Ishihara, K.
    • Publisher
      Nova Science Publishers(in press)
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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