| Project/Area Number |
22592317
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
SAITO Atsushi 東京歯科大学, 歯学部, 教授 (60266559)
|
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| Co-Investigator(Kenkyū-buntansha) |
ISHIHARA Kazuyuki 東京歯科大学, 歯学部, 教授 (00212910)
INAGAKI Satoru 東京歯科大学, 歯学部, 助教 (20385165)
KOKUBU Eitoyo 東京歯科大学, 歯学部, 助教 (70453785)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | 歯周炎 / 歯周病原細菌 / 侵入 / バイオフィルム / 細胞侵入 / 歯肉上皮細胞 / Porphyromonas gingivalis / Fusobacterium nucleatum / 歯周病 / 歯周病原性菌 |
|---|
| Research Abstract |
In order to gain insight into the mechanism and significance of host cell invasion by periodontal bacteria in polymicrobial infecti on, we assessedPorphyromonas gingivalis invasion of gingival epithelial cells enhanced by Fusobacterium nucleatum. Confocal scanning laser microscopy and antibiotic protection assay were used to study the role of P. gingivalis invasin (SerB) in this phenomena. The results from the experiment using SerB-mutant strain showed that the P. gingivalis invasin does not play a major role in this phenomenon. It was demonstrated that lipid raft was involved in fusobacterial enhancement of P. gingivalis invasion.
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