Foundation of alveolar bone regenerative therapy strategy used new synthetic peptide
| Project/Area Number |
22592324
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
UEDA Masatoshi 大阪歯科大学, 歯学部, 名誉教授 (00067117)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Akio 大阪歯科大学, 歯学部, 教授 (10121823)
TAGUCHI Yoichiro 大阪歯科大学, 歯学部, 講師 (60434792)
TOMINAGA Kazuya 大阪歯科大学, 歯学部, 講師 (80278572)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | エナメル基質タンパク / 歯根膜細胞 / 歯周組織再生 / エナメルマトリックスデリバティブ / 合成ペプチド / 骨髄細胞 / 硬組織分化誘導 / エナメルマトリックス / 歯周病 / 再生治療 / ペプチド |
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| Research Abstract |
One method of treating periodontitis is to use EmdogainR, a material derived from the tooth germ of juvenile swine that promotes periodontal tissue regeneration, including the formation of hard tissue as cementum, alveolar bone. The use of EmdogainR is therefore established in the field of periodontal regenerative therapy. However, because of its swine origin, some patients choose not to be treated with EmdogainR. The active component of EmdogainR has been shown to be a peptide whose sequence corresponds to an amelogenin II precursor. As such, this peptide may function as a growth factor to stimulate cell differentiation and tissue regeneration. In this study, we characterized the effects of the synthetic EmdogainR-derived peptide on the proliferation, adhesion, migration and differentiation of periodontal ligament fibroblasts (HPdLFs), which display properties similar to mesenchymal stem cells, and Sprague-Dawley rat bone marrow (RBM) cells. Compared to cells not treated with tesynthetic EmdogainR-derived peptide, treated cells showed increased proliferation, initial adhesion, and chemotactic activity. The optimum peptide concentration that stimulated these activities was determined to be 100 ng/ml. We next investigated the effects of the peptide at a concentration of 100 ng/ml on osteogenesis and cementogenesis in HPdLF cells by assaying alkaline phosphatase activity, osteocalcin production, and mineralization. Taken together, our data suggest that the EmdogainR-derived peptide stimulates periodontal hard tissue regeneration by stimulating the proliferation, adhesion, and migration of mesenchymal stem cells.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] ヒト歯肉上皮細胞に及ぼす影響から考えるエナメルマトリックスデリバティブ由来合成ペプチドの有用性2010
Author(s)
田口洋一郎,安井菜津希,富永和也,橋本典也,至田宗泰,玉置敏夫,北條博一,武田昭二,林宏行,田中昭男,上田雅俊
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Journal Title
日本歯科保存学雑誌
Volume: 53
Pages: 449-456
Related Report
Peer Reviewed
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