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Foundation of alveolar bone regenerative therapy strategy used new synthetic peptide

Research Project

Project/Area Number 22592324
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionOsaka Dental University

Principal Investigator

UEDA Masatoshi  大阪歯科大学, 歯学部, 名誉教授 (00067117)

Co-Investigator(Kenkyū-buntansha) TANAKA Akio  大阪歯科大学, 歯学部, 教授 (10121823)
TAGUCHI Yoichiro  大阪歯科大学, 歯学部, 講師 (60434792)
TOMINAGA Kazuya  大阪歯科大学, 歯学部, 講師 (80278572)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsエナメル基質タンパク / 歯根膜細胞 / 歯周組織再生 / エナメルマトリックスデリバティブ / 合成ペプチド / 骨髄細胞 / 硬組織分化誘導 / エナメルマトリックス / 歯周病 / 再生治療 / ペプチド
Research Abstract

One method of treating periodontitis is to use EmdogainR, a material derived from the tooth germ of juvenile swine that promotes periodontal tissue regeneration, including the formation of hard tissue as cementum, alveolar bone. The use of EmdogainR is therefore established in the field of periodontal regenerative therapy. However, because of its swine origin, some patients choose not to be treated with EmdogainR. The active component of EmdogainR has been shown to be a peptide whose sequence corresponds to an amelogenin II precursor. As such, this peptide may function as a growth factor to stimulate cell differentiation and tissue regeneration. In this study, we characterized the effects of the synthetic EmdogainR-derived peptide on the proliferation, adhesion, migration and differentiation of periodontal ligament fibroblasts (HPdLFs), which display properties similar to mesenchymal stem cells, and Sprague-Dawley rat bone marrow (RBM) cells. Compared to cells not treated with tesynthetic EmdogainR-derived peptide, treated cells showed increased proliferation, initial adhesion, and chemotactic activity. The optimum peptide concentration that stimulated these activities was determined to be 100 ng/ml. We next investigated the effects of the peptide at a concentration of 100 ng/ml on osteogenesis and cementogenesis in HPdLF cells by assaying alkaline phosphatase activity, osteocalcin production, and mineralization. Taken together, our data suggest that the EmdogainR-derived peptide stimulates periodontal hard tissue regeneration by stimulating the proliferation, adhesion, and migration of mesenchymal stem cells.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2013 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (12 results) Book (1 results)

  • [Journal Article] Hard Tissue Formation by Human Periodontal Ligament Fibroblast Cells Treated With an EmdogainR-derived Oligopeptides in vitro2012

    • Author(s)
      Yoichiro Taguchi, Saitatsu Takahashi,Kazuya Tominaga, Hirohito Kato, Satoshi Komasa, Natsuki Yasui,Muneyasu Shida, Hiroyuki Hayashi, Akio Tanaka and Makoto Umeda
    • Journal Title

      Journal of Hard Tissue Biology

      Volume: 21 Pages: 375-384

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] ヒト培養歯根膜細胞の増殖,接着,遊走に及ぼすエナメルマトリックスデリバティブ誘導体由来合成ペプチドの影響2012

    • Author(s)
      田口洋一郎,高橋宰達,富永和也,小正聡,至田宗泰,林宏行,田中昭男,梅田誠
    • Journal Title

      日本歯科保存学雑誌

      Volume: 55 Pages: 227-235

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biological Effects of EmdogainR-derived Oligopeptideson Rat Bone Marrow Cells in Vitro2012

    • Author(s)
      Natsuki Yasui, Yoichiro Taguchi, Akio Tanaka, Masatoshi Ueda and Makoto Umeda.
    • Journal Title

      Journal of Oral Tissue Engineering

      Volume: 9 Pages: 126-135

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hard Tissue Formation by Human Periodontal Ligament Fibroblast Cells Treated With an Emdogain-derived Oligopeptide in vitro.2012

    • Author(s)
      Yoichiro Taguchi, Natsuki Yasui, Saitatsu Takahashi, Kazuya Tominaga, Hirohito Kato, Satoshi Komasa, Muneyasu Shida, Hiroyuki Hayashi, Akio Tanaka, Makoto Umeda.
    • Journal Title

      Journal of Hard Tissue Biology

      Volume: 21 Pages: 375-384

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ヒト培養歯根膜細胞の増殖,接着,遊走に及ぼすエナメルマトリックスデリバティブ誘導体由来の合成ペプチドの影響2012

    • Author(s)
      田口洋一郎,高橋宰達,富永和也,小正聡,至田宗泰,林宏行,田中昭男,梅田誠.
    • Journal Title

      日本歯科保存学雑誌

      Volume: 55 Pages: 227-235

    • NAID

      110009480000

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biological Effects of Emdogain-derived Oligopeptides on Rat Bone Marrow Cells in Vitro2012

    • Author(s)
      Natsuki Yasui, Yoichiro Taguchi, Akio Tanaka, Masatoshi Ueda, Makoto Umeda
    • Journal Title

      Journal of Oral Tissue Engineering

      Volume: 9 Pages: 126-135

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of enamel matrix derivatives on periodontal tissue regeneration; focusing on radiographic observation.2011

    • Author(s)
      Naoko Yoshikawa, Yoshio Kotsu, Chizuko Ogata, Yoichiro Taguchi, Toshikatsu Koike, Yukiko Tomii, Shoichiro Terada, Mika Ueda, Michiko Noguchi, Kazuki Ogiso and Masatoshi Ueda.
    • Journal Title

      Journal of Osaka Dental University

      Volume: 45 Pages: 265-268

    • NAID

      110008761453

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] ヒト歯肉上皮細胞に及ぼす影響から考えるエナメルマトリックスデリバティブ由来合成ペプチドの有用性2010

    • Author(s)
      田口洋一郎,安井菜津希,富永和也,橋本典也,至田宗泰,玉置敏夫,北條博一,武田昭二,林宏行,田中昭男,上田雅俊
    • Journal Title

      日本歯科保存学雑誌

      Volume: 53 Pages: 449-456

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] ヒト歯肉上皮細胞に及ぼす影響から考えるエナメルマトリックスデリバティブ誘導体由来合成ペプチドの有用性2010

    • Author(s)
      田口洋一郎
    • Journal Title

      日本歯科保存学雑誌

      Volume: 53 Pages: 449-456

    • NAID

      110008006914

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Fibroblast Cells Treated With an Emdogain derived Oligopeptide in vitro2013

    • Author(s)
      Yoichiro Taguchi, Saitatsu Takahashi,Kazuya Tominaga, Satoshi Komasa, Natsuki Yasui, Akio Tanaka, Makoto Umeda
    • Organizer
      91th Annual Meeting of International Associationfor Dental Research
    • Place of Presentation
      Seattle, USA.
    • Year and Date
      2013-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト歯根膜細胞の増殖,接着,遊走能に対するEMD由来合成ペプチドの影響2012

    • Author(s)
      高橋宰達,田口洋一郎,小正聡,奥田麻貴子,安井菜津希,田中昭男,梅田誠
    • Organizer
      日本歯科保存学会2012年度春季学術大会(第136回)
    • Place of Presentation
      那覇市
    • Year and Date
      2012-06-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 各種細胞に及ぼす影響から考えるEMD由来合成ペプチドの有用性2012

    • Author(s)
      田口洋一郎
    • Organizer
      日本歯科医学会総会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト歯根膜細胞の増殖,接着,遊走能に対するEMD由来合成ペプチドの影響2012

    • Author(s)
      高橋宰達
    • Organizer
      日本歯科保存学会平成24年度春季学術大会
    • Place of Presentation
      沖縄コンベンションセンター
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト歯根膜由来幹細胞の免疫組織化学的観察および骨芽細胞分化能の検討2012

    • Author(s)
      嘉藤弘仁
    • Organizer
      日本歯周病学会平成24年度春季学術大会
    • Place of Presentation
      札幌コンベンションセンター
    • Related Report
      2012 Annual Research Report
  • [Presentation] ラット骨髄細胞に対するエナメル基質タンパク由来合成ペプチドの初期硬組織分化誘導2011

    • Author(s)
      安井菜津希,田口洋一郎,富永和也,高橋宰達,奥田麻貴子,木村大輔,片山暢仁,南堂百映,田中昭男,上田雅俊
    • Organizer
      第54回日本歯周病学会秋季学術大会
    • Place of Presentation
      下関市
    • Year and Date
      2011-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] ラット骨髄細胞に対するエナメル基質タンパク由来合成ペプチドの初期硬組織分化誘導2011

    • Author(s)
      安井菜津希, 田口洋一郎, 富永和也, 田中昭男, 上田雅俊, 他
    • Organizer
      日本歯周病学会第54回秋季学術大会
    • Place of Presentation
      海峡メッセ下関
    • Year and Date
      2011-09-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] In vitro effects of EMD-derived oligopeptides on human epithelial cells2011

    • Author(s)
      Natsuki Yasui, Yoichiro Taguchi, Kazuya Tominaga, Yoshiya Hashimoto, Shoichiro Terada, Nobuhiro Shigematsu, Shoji Terada, Akio Tanaka, Masatoshi Ueda
    • Organizer
      89th Annual Meeting of International Association for Dental Research
    • Place of Presentation
      San Diego, USA.
    • Year and Date
      2011-03-16
    • Related Report
      2012 Final Research Report
  • [Presentation] ラット骨髄細胞に対するEMD由来合成ペプチドの有用性2010

    • Author(s)
      安井菜津希,田口洋一郎,富永和也,高橋宰達,田中昭男,上田雅俊
    • Organizer
      第53回日本歯周病学会秋季学術大会
    • Place of Presentation
      高松市
    • Year and Date
      2010-09-19
    • Related Report
      2012 Final Research Report
  • [Presentation] In vitro effects of EMD-derived oligopeptides on human epithelial cells.2010

    • Author(s)
      Yasui N
    • Organizer
      International Association for Dental Research 2010 Annual Meeting
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2010-07-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] EMD由来合成ペプチドのヒト歯肉上皮細胞に及ぼす影響から考える至適濃度2010

    • Author(s)
      田口洋一郎,安井菜津希,富永和也,寺田昌一郎,田中昭男,上田雅俊
    • Organizer
      特定非営利活動法人日本歯科保存学会2010年度春季学術大会(第132回)
    • Place of Presentation
      熊本市
    • Year and Date
      2010-06-04
    • Related Report
      2012 Final Research Report
  • [Presentation] Emdogain由来新規合成ペプチドによるヒト歯根膜由来幹細胞に対する効果についての検討

    • Author(s)
      嘉藤弘仁
    • Organizer
      日本歯周病学会平成24年度秋季学術大会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2012 Annual Research Report
  • [Book] 歯科衛生士講座歯周病の基礎と臨床第2版2011

    • Author(s)
      上田雅俊, 田中昭男, 前田勝正, 他
    • Total Pages
      267
    • Publisher
      永末書店
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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