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The monitoring system of incipient caries, and its special attention o bacterial dynamics in dental plaque biofilm

Research Project

Project/Area Number 22592330
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Social dentistry
Research InstitutionNiigata University

Principal Investigator

MATSUYAMA Junko  新潟大学, 医歯学系, 助教 (30293236)

Co-Investigator(Kenkyū-buntansha) TAKENAKA Syoji  新潟大学, 医歯学系, 助教 (50313549)
SATO Takuichi  東北大学, 歯学研究科(研究院), 講師 (10303132)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords口腔衛生学 / 初期齲蝕の予防 / 歯垢 / バイオフィルム / 歯垢バイオフィルム / 初期齲蝕 / 細菌 / 小児 / 齲蝕 / 歯学 / 口腔細菌 / PCR / 16S rRNA / 齲蝕予防
Research Abstract

Plaque biofilm of humans was investigated using a rapid microbial cell counters and anaerobic cultures. On the samples of around 10^5 CFU counted, the same levels of bacterial counts were observed by the anaerobic cultures. While, indigenous plaque microbiota of mice was also analysed, and as a result, Enterococcus faecalis, Escherichia coli, Lactococcus garvieae were detected as predominant bacteria.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (55 results)

All 2013 2012 2011 2010 Other

All Journal Article (24 results) (of which Peer Reviewed: 22 results) Presentation (22 results) Book (8 results) Remarks (1 results)

  • [Journal Article] Responses of infected dental pulp to αTCP containing antimicrobials in rat molars2013

    • Author(s)
      Sato T, Kenmotsu S, Nakakura-Ohshima K, Takahashi N, Ohshima H
    • Journal Title

      Arch Histol Cytol

      Volume: 75: in press

    • NAID

      130005087018

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quantification and identification of bacteria in acrylic resin denture bases and dento-maxillary obturator- prostheses2012

    • Author(s)
      Takeuchi Y, Nakajo K, Sato T, Koyama S, Sasaki K, Takahashi N
    • Journal Title

      Am J Dent

      Volume: 25 Pages: 171-175

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid quantification of bacteria in infected root canals using fluorescence reagents and a membrane filter: A pilot study on its clinical application to the evaluation of the outcomes of endodontic treatment2012

    • Author(s)
      Sato T, Yamaki K, Ishida N, Shoji M, Sato E, Abiko Y, Hashimoto K, Takeuchi Y, Matsuyama J, Shimauchi H, Takahashi N
    • Journal Title

      Int J Dent

      Pages: 4-4

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microbial composition of dental plaque microflora on first molars with orthodontic bands2012

    • Author(s)
      Komori R, Sato T, Takano-Yamamoto T, Takahashi N
    • Journal Title

      J Oral Biosci

      Volume: 54 Pages: 107-112

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cultivable anaerobic microbiota of infected root canals2012

    • Author(s)
      Sato T, Yamaki K ,Ishida N, Hashimoto K, Takeuchi Y, Shoji M, Sato E, Matsuyama J, Shimauchi H, Takahashi N
    • Journal Title

      Int J Dent

      Pages: 5-5

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microflora profiling of infected root canal before and after treatment using culture-independent methods2012

    • Author(s)
      Ito Y, Sato T, Yamaki K, Mayanagi G, Hashimoto K, Shimauchi H, Takahashi N
    • Journal Title

      J Microbiol

      Volume: 50(1) Issue: 1 Pages: 58-62

    • DOI

      10.1007/s12275-012-0459-4

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Current and future strategies for the control of mature oral biofilms-Shift of a focus from bacteria-targeting to a matrix-targeting approach-2012

    • Author(s)
      Takenaka S, Ohshima H, Osumi T, Okiji T
    • Journal Title

      J Oral Biosci

      Volume: 54 Issue: 4 Pages: 173-179

    • DOI

      10.1016/j.job.2012.09.002

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid quantification of bacteria in infected root canals using fluorescence filter : A pilot study on its clinical application to the evaluation of the outcomes of endodontic treatment2012

    • Author(s)
      Sato T, Yamaki K, Ishida N, Shoji M, Sato E, Abiko Y, Hashimoto K, Takeuchi Y, Matsuyama J, Shimauchi H, Takahashi N
    • Journal Title

      Int J Dent

      Volume: Volume 2012(Article ID 172935) Pages: 4-4

    • DOI

      10.1155/2012/172935

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cultivable anaerobic microbiota of infected root canals2012

    • Author(s)
      Sato T, Yamaki K, Ishida N, Hashimoto K, Takeuchi Y, Shoji M, Sato E, Matsuyama J, Shimauchi H, Takahashi N
    • Journal Title

      Int J Dent

      Volume: Volume 2012(Article ID 609689) Pages: 5-5

    • DOI

      10.1155/2012/609689

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbial composition of dental plaque microflora on first molars with orthodontic bands2012

    • Author(s)
      Komori R, Sato T, Takano-Yamamoto T, Takahashi N
    • Journal Title

      J Oral Biosci

      Volume: 54 Issue: 2 Pages: 107-112

    • DOI

      10.1016/j.job.2012.01.009

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantification and identification of bacteria in acrylic resin denture bases and dento-maxillary obturator-prostheses.2012

    • Author(s)
      Takeuchi Y
    • Journal Title

      Am J Dent

      Volume: 25 Pages: 171-175

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Responses of infected dental pulp to αTCP containing antimicrobials in rat molars2012

    • Author(s)
      Takuichi Sato
    • Journal Title

      Arch Histol Cytol

      Volume: 75(in press)

    • NAID

      130005087018

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantification and identification of bacteria in acrylic resin denture bases and dento-maxillary obturator-prostheses2012

    • Author(s)
      Yasuhisa Takeuchi
    • Journal Title

      Am J Dent

      Volume: 25(in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbial composition and acidogenic potential of dental plaque microflora on first molars with orthodontic bands and brackets2012

    • Author(s)
      Ryo Komori
    • Journal Title

      J Oral Biosci

      Volume: 55(in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cultivable anaerobic microbiota of infected root canals2012

    • Author(s)
      Takuichi Sato
    • Journal Title

      Int J Dent

      Volume: 2012

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid quantification of bacteria in infected root canals using fluorescence filter2012

    • Author(s)
      Takuichi Sato
    • Journal Title

      Int J Dent

      Volume: 2012(in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Profiling of dental plaque microflora on root caries lesions and the protein-denaturing activity of these bacteria2011

    • Author(s)
      Hashimoto K, Sato T, Shimauchi H, Takahashi N
    • Journal Title

      Am J Dent

      Volume: 24 Pages: 295-299

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection and identification of non-Candida albicans species in human oral lichen planus2011

    • Author(s)
      Masaki M, Sato T, Sugawara Y, Sasano T, Takahashi N
    • Journal Title

      Microbiol Immunol

      Volume: 55(1) Issue: 1 Pages: 66-70

    • DOI

      10.1111/j.1348-0421.2010.00285.x

    • NAID

      10031121442

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] An in vitro model for a packed bacterial mass formation with special attention to less interstitial spaces2011

    • Author(s)
      Al-Shawafi H, Uematsu H, Takenaka S, Hoshino E
    • Journal Title

      Niigata Dent J

      Volume: 41 Pages: 13-20

    • NAID

      120006761808

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection and identification of non-Candida albicans species in human oral lichen planus.2011

    • Author(s)
      Mika Masaki, Takuichi Sato
    • Journal Title

      Microbiol Immunol

      Volume: 55 Pages: 66-70

    • NAID

      10031121442

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 口腔内細菌叢のコントロール2010

    • Author(s)
      河村好章,長谷川義明,佐藤拓一
    • Journal Title

      ファルマシア

      Volume: 46 Pages: 929-933

    • Related Report
      2012 Final Research Report
  • [Journal Article] Host beta-globin gene fragments in crevicular fluid as a biomarker in periodontal health and disease2010

    • Author(s)
      Thaweboon B, Laohapand P, Amornchat C, Matsuyama J, Sato T, Nunez PP, Uematsu H, Hoshino E
    • Journal Title

      J Periodontal Res

      Volume: 45 Issue: 1 Pages: 38-44

    • DOI

      10.1111/j.1600-0765.2008.01197.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Profiling of subgingival plaque biofilm microflora from periodontally healthy subjects and from subjects with periodontitis using quantitative real-time PCR2010

    • Author(s)
      Abiko Y, Sato T, Mayanagi G, Takahashi N
    • Journal Title

      J Periodontal Res

      Volume: 45 Pages: 389-395

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Profiling of subgingival plaque biofilm microflora from periodontally healthy subjects and from subjects with periodontitis using quantitative real-time PCR.2010

    • Author(s)
      Yuki Abiko, Takuichi Sato
    • Journal Title

      J Periodontal Res

      Volume: 45 Pages: 389-395

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 蛍光フィルターを利用した、感染根管内細菌の迅速定量検出法2012

    • Author(s)
      庄司恵,佐藤愛美加,石田直子,八巻惠子,佐藤拓一,高橋信博
    • Organizer
      第22回日本歯科医学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2012-11-11
    • Related Report
      2012 Final Research Report
  • [Presentation] 口腔バイオフィルムの分子生物学的プロファイリング-メタゲノム解析-2012

    • Author(s)
      佐藤拓一,河村好章,八巻惠子,島内英俊,高橋信博
    • Organizer
      第22回日本歯科医学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2012-11-10
    • Related Report
      2012 Final Research Report
  • [Presentation] マウス口腔内プラーク常在菌叢の網羅的解析2012

    • Author(s)
      松山順子,佐藤拓一,Angela Quispe-Salcedo,石田直子,高橋信博,大島勇人
    • Organizer
      第54回歯科基礎医学会学術大会
    • Place of Presentation
      郡山
    • Year and Date
      2012-09-16
    • Related Report
      2012 Final Research Report
  • [Presentation] The oral microbiome and biofilm research: new concepts and new approaches by presentation by young researchers.2012

    • Author(s)
      Sato T,Ohshima T,Miyakawa H,Hamada N
    • Organizer
      第54回歯科基礎医学会学術大会サテライトシンポジウム
    • Place of Presentation
      郡山
    • Year and Date
      2012-09-14
    • Related Report
      2012 Final Research Report
  • [Presentation] マウス口腔内プラークの網羅的解析2012

    • Author(s)
      松山順子
    • Organizer
      歯科基礎医学会総会
    • Place of Presentation
      郡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高齢者の歯肉縁下プラーク細菌叢: Porphyromonas gingivalisの定量解析とfimA遺伝子型タイピング2011

    • Author(s)
      安彦友希
    • Organizer
      第53回歯科基礎医学会学術大会
    • Place of Presentation
      岐阜長良川国際会議場
    • Year and Date
      2011-10-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] 感染根管内細菌叢のpyrosequencing 法によるメタゲノム解析2011

    • Author(s)
      佐藤拓一,河村好章,八巻惠子,島内英俊,高橋信博
    • Organizer
      第53回歯科基礎医学会学術大会
    • Place of Presentation
      岐阜
    • Year and Date
      2011-10-01
    • Related Report
      2012 Final Research Report
  • [Presentation] 感染根管内細菌叢のpyrosequencing法によるメタゲノム解析2011

    • Author(s)
      佐藤拓一
    • Organizer
      第53回歯科基礎医学会学術大会
    • Place of Presentation
      岐阜長良川国際会議場
    • Year and Date
      2011-10-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] Intensive perioperative oral care prevents postoperative pneumonia after lung resection2011

    • Author(s)
      Hoshikawa Y, Tanda N, Endo H, Sato T, Hasegawa A, Kondo T
    • Organizer
      American Thoracic Society 2011 International Conference
    • Place of Presentation
      Denver, Colorado, USA
    • Year and Date
      2011-05-17
    • Related Report
      2012 Final Research Report
  • [Presentation] Subgingival plaque biofilm microflora of elderly subjects: quantitative analysis of Porphyromonas gingivalis and genotyping of its virulence- associated fimA2011

    • Author(s)
      Abiko Y, Sato T, Sakashita R, Takahashi N
    • Organizer
      The 4th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      2011-03-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Silent aspiration of oral bacteria - microbiological analysis of intraoperative bronchial fluids from patients with pulmonary carcinoma2011

    • Author(s)
      Hasegawa A, Sato T, Hoshikawa Y, Kondo T, Takahashi N
    • Organizer
      The 4th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      2011-03-08
    • Related Report
      2012 Final Research Report
  • [Presentation] A method for quantitatively evaluating plaque biofilm removing capacity of a dental water jet using EPMA2011

    • Author(s)
      Kato K, Tamura K, Nakagaki H, Sakakibara S, Ou Y, Matsumoto S, Fujita K, Sato T
    • Organizer
      The 4th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      2011-03-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Quantification and identification of bacteria in the maxillary obturator- prostheses2011

    • Author(s)
      Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Koyama S, Sasaki K, Takahashi N
    • Organizer
      The 4th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      2011-03-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Change in infected root canal microflora during the course of root canal treatment2011

    • Author(s)
      Yamaki K, Sato T, Shimauchi H
    • Organizer
      The 4th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      2011-03-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Community oral health promotion program fostering self-management for elderly people2011

    • Author(s)
      Nishihira T, Nishitani M, Sato T, Abiko Y, Matsushita K, Hamada M, Sakashita R
    • Organizer
      The 4th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      2011-03-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Change in infected root canal microflora during the course of root canal therapy.2011

    • Author(s)
      Keiko Yamaki, Junko Matsuyama, Takuichi Sato
    • Organizer
      The 4th International Symposium for Interface Oral Health Science
    • Place of Presentation
      仙台
    • Year and Date
      2011-03-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 口腔フローラのプロファイリング2010

    • Author(s)
      佐藤拓一
    • Organizer
      第58回東北大学歯学会
    • Place of Presentation
      仙台
    • Year and Date
      2010-12-10
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] 高齢肺切除症例に対する周術期口腔ケアの試み2010

    • Author(s)
      丹田奈緒子,星川康,遠藤ひとみ,佐藤拓一,細川亮一,田浦勝彦,齋藤恵一,井川恭子,鈴木淳,小関健由
    • Organizer
      第59回日本口腔衛生学会・総会
    • Place of Presentation
      新潟
    • Year and Date
      2010-10-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Silent aspiration of oral bacteria in elderly subjects2010

    • Author(s)
      Hasegawa A, Sato T, Hoshikawa Y, Abiko Y, Kondo T, Takahashi N
    • Organizer
      The 88th IADR
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2010-07-17
    • Related Report
      2012 Final Research Report
  • [Presentation] Silent aspiration of oral bacteria in elderly subjects.2010

    • Author(s)
      Ayako Hasegawa, Takuichi Sato
    • Organizer
      第88回国際歯科研究学会(IADR)
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2010-07-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高齢者肺切除術後肺炎発症機序の解明と予防策2010

    • Author(s)
      星川康,松田安史,遠藤ひとみ,丹田奈緒子,佐藤拓一,岡田克典,近藤丘
    • Organizer
      第27回日本呼吸器外科学会総会シンポジウム
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] 周術期専門的口腔ケア介入の肺切除術後肺炎予防効果2010

    • Author(s)
      星川康,遠藤ひとみ,丹田奈緒子,佐藤拓一,長谷川彩子,野田雅史,佐渡哲,大石久,前田寿美子,桜田晃,遠藤千顕,岡田克典,近藤丘
    • Organizer
      第50回日本呼吸器学会学術講演会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Book] 口腔内微生物叢と歯面のバイオフィルムIn:デンタルカリエス-その病態とクリニカルマネージメント、第2版2013

    • Author(s)
      佐藤拓一
    • Publisher
      医歯薬出版
    • Related Report
      2012 Final Research Report
  • [Book] Subgingival plaque biofilm microflora of elderly subjects: quantitative analysis of Porphyromonas gingivalis and genotyping of its virulence-associated fimA. Interface Oral Health Science 20112012

    • Author(s)
      Abiko Y, Sato T, Sakashita R, Takahashi N: K. Sasaki, O. Suzuki, N. Takahashi (eds.)
    • Publisher
      Springer, New York
    • Related Report
      2012 Final Research Report
  • [Book] Silent aspiration of oral bacteria Microbiological analysis of intraoperative bronchial fluids from patients with pulmonary carcinoma. Interface Oral Health Science 20112012

    • Author(s)
      Hasegawa A, Sato T, Hoshikawa Y, Kondo T, Takahashi N: K. Sasaki, O. Suzuki, N. Takahashi (eds.)
    • Publisher
      Springer, New York
    • Related Report
      2012 Final Research Report
  • [Book] A method for quantitatively evaluating plaque biofilm removing capacity of a dental water jet using EPMA. Interface Oral Health Science 20112012

    • Author(s)
      Kato K, Tamura K, Nakagaki H, Sakakibara S, Ou Y, Matsumoto S, Fujita K, Sato T: K. Sasaki, O. Suzuki, N. Takahashi (eds.)
    • Publisher
      Springer, New York
    • Related Report
      2012 Final Research Report
  • [Book] Quantification and identification of bacteria in the maxillary obturator- prostheses. Interface Oral Health Science 20112012

    • Author(s)
      Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Koyama S, Sasaki K, Takahashi N: K. Sasaki, O. Suzuki, N. Takahashi (eds.)
    • Publisher
      Springer, New York
    • Related Report
      2012 Final Research Report
  • [Book] Change in infected root canal microflora during the course of root canal therapy. Interface Oral Health Science 20112012

    • Author(s)
      Yamaki K, Sato T, Hasegawa A, Abiko Y, Hashimoto K, Takeuchi Y, Matsuyama J, Shimauchi H, Takahashi N: K. Sasaki, O. Suzuki, N. Takahashi (eds.)
    • Publisher
      Springer, New York
    • Related Report
      2012 Final Research Report
  • [Book] Community oral health promotion program fostering self-management for elderly people. Interface Oral Health Science 20112012

    • Author(s)
      Nishihira T, Nishitani M, Sato T, Abiko Y, Matsushita K, Hamada M, Sakashita R: K. Sasaki, O. Suzuki, N. Takahashi (eds.)
    • Publisher
      Springer, New York
    • Related Report
      2012 Final Research Report
  • [Book] Interface Oral Health Science 20112012

    • Author(s)
      Keiko Yamaki
    • Publisher
      Springer
    • Related Report
      2011 Annual Research Report
  • [Remarks] 研究者又は所属研究機関が作成した研究内容又は研究成果に関するwebページがある場合は、URLを記載すること

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-11-30   Modified: 2019-07-29  

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