Study on structure and function of novel tachykinin peptides

• Project/Area Number: 22600008
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 疼痛学
• Research Institution: University of Miyazaki
• Principal Investigator: NISHIMORI Toshikazu 宮崎大学, 医学部, 教授 (20112211)
• Co-Investigator(Kenkyū-buntansha): IKEDA Tetsuya 宮崎大学, 医学部, 准教授 (20264369)
• Research Collaborator: NAKAYAMA Rumi 宮崎大学, 医学部, 研究員 (00609034)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 疼痛 / ペプチド / 脊髄 / 神経薬理

Research Abstract

Endokinins are novel tachykinin peptides, in which endokinin C/D (EKC/D) has the inhibitory effect on noxious stimulation. Replacement of EKC/D with D-type amino acid remarkably extended this effect. Also, intrathecal administration of hemokinin-1, another novel tachykinin peptide, did not induce thermal hyperalgesia. This effect of hemokinin-1 was caused by 3 amino acids consisting ofN-terminal region of this peptide.

Research Products

• [Journal Article] New tachykinin peptides and nociception. (2013)
  • Author(s): T. Nishimori, R. Naono-Nakayama, T. Ikeda
  • Journal Title: Japanese Dental Sci. Rev. Volume: 49 Issue: 1 Pages: 27-34
  • DOI: 10.1016/j.jdsr.2012.11.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] New tachykinin peptides and nociception (2012)
  • Author(s): Nishimori T, Naono-Nakayama R, Ikeda T
  • Journal Title: Jpn dent sci rev. Volume: 49 Pages: 27-34
  • NAID: 10031201720
  • URL: http://dx.doi.org/10.1016/j.jdsr.2012.11.002
• [Journal Article] Pharmacological characteristics of endokinin C/D-derived peptides in nociceptive and inflammatory processing in rats. (2011)
  • Author(s): Naono-Nakayama R, Sunakawa N, Ikeda T, Matsushima O, Nishimori T
  • Journal Title: Peptides Volume: 32(12) Issue: 12 Pages: 2407-2417
  • DOI: 10.1016/j.peptides.2011.10.009
• [Journal Article] Knockdown of the tachykinin neurokinin 1 receptor by intrathecal administration of small interfering RNA in rats. (2011)
  • Author(s): Naono-Nakayama R, Sunakawa N, Ikeda T, Nishimori T
  • Journal Title: Eur J Pharmacol. Volume: 670(2-3) Issue: 2-3 Pages: 448-457
  • DOI: 10.1016/j.ejphar.2011.09.035
• [Journal Article] Pharmacological characteristics of endokinin C/D-derived peptides in nociceptive and inflammatory processing in rats (2011)
  • Author(s): Naono-Nakayama R, Sunakawa N, Ikeda T, Matsushima O, Nishimori T
  • Journal Title: Peptides Volume: 32 Pages: 2407-2417
  • Peer Reviewed
• [Journal Article] Knockdown of the tachykinin neurokinin 1 receptor by intrathecal administration of small interfering RNA in rats (2011)
  • Author(s): Naono-Nakayama R, Sunakawa N, Ikeda T, Nishimori T
  • Journal Title: Eur J Pharmacol Volume: 670 Pages: 448-457
  • Peer Reviewed
• [Journal Article] Subcutaneous injection of endokinin C/D attenuates carrageenan-induced inflammation. (2010)
  • Author(s): Naono-Nakayama R, Sunakawa N, Ikeda T, Matsushima O, Nishimori T
  • Journal Title: Peptides Volume: 31(9) Issue: 9 Pages: 1767-1771
  • DOI: 10.1016/j.peptides.2010.05.019
• [Journal Article] The amino-terminal region of hemokinin-1 regulates the induction of thermal hyperalgesia in rats. (2010)
  • Author(s): Sunakawa N, Naono R, Ikeda T, Matsushima O, Sakoda S, Nishimori T
  • Journal Title: Neuropeptides Volume: 44(3) Issue: 3 Pages: 273-278
  • DOI: 10.1016/j.npep.2010.01.003
• [Journal Article] NMDA and AMPA receptors contribute to the maintenance of substance P-induced thermal hyperalgesia. (2010)
  • Author(s): Nakayama T, Naono R, Ikeda T, Nishimori T
  • Journal Title: Neurosci Res. Volume: 67(1) Issue: 1 Pages: 18-24
  • DOI: 10.1016/j.neures.2010.01.006
• [Journal Article] NMDA and AMPA receptors contribute to the maintenance of substance P-induced thermal hyperalgesia. (2010)
  • Author(s): Nakayama T, Naono R, Ikeda T, Nishimori T.
  • Journal Title: Neurosci Res. Volume: 67 Pages: 18-24
  • Peer Reviewed
• [Journal Article] The amino-terminal region of hemokinin-1 regulates the induction of thermal hyperalgesia in rats. (2010)
  • Author(s): Sunakawa N, Naono R, Ikeda T, Matsushima O, Sakoda S, Nishimori T.
  • Journal Title: Neuropeptides. Volume: 44 Pages: 273-278
  • Peer Reviewed
• [Journal Article] Subcutaneous injection of endokinin C/D attenuates carrageenan-induced inflammation. (2010)
  • Author(s): Naono-Nakayama R, Sunakawa N, Ikeda T, Matsushima O, Nishimori T.
  • Journal Title: Peptides. Volume: 31 Pages: 1767-1771
  • Peer Reviewed
• [Presentation] Effect of a fragment peptide derived from hemokinin-1 on histamine- or serotonin-induced scratching (2012)
  • Author(s): Naono-Nakayama R, Ikeda T, Takamiya K, Sameshima H, Nishimori T
  • Organizer: Society for Neuroscience, 42th Annual Meeting
  • Place of Presentation: New Orleans, USA.
• [Presentation] Effect of a fragment peptide derived from hemokinin-1 on histamine- or serotonin-induced scratching. (2012)
  • Author(s): Naono-Nakayama R, Ikeda T, Takamiya K, Sameshima H, Nishimori T.
  • Organizer: Society for Neuroscience, 42th Annual Meeting
  • Place of Presentation: New Orleans
• [Presentation] 疼痛およびかゆみに対するヘモキニン-1由来フラグメントペプチドの効果. (2011)
  • Author(s): 中山-直野留美, 池田哲也, 西森利數
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] 疼痛およびかゆみに対するヘモキニン-1由来フラグメントペプチドの効果 (2011)
  • Author(s): 中山-直野留美, 池田哲也, 西森利數
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Patent(Industrial Property Rights)] ヘモキニンー1受容体及びヘモキニンー1由来ペプチド (2012)
  • Inventor(s): 西森利數
  • Industrial Property Rights Holder: 西森利數
  • Filing Date: 2012-03-01
  • Overseas
• [Patent(Industrial Property Rights)] エンドキニン C/D 由来のペプチド (2010)
  • Inventor(s): 西森利數
  • Industrial Property Rights Holder: 西森利數
  • Filing Date: 2010-12-09
  • Overseas
• [Patent(Industrial Property Rights)] エンドキニンC/D由来のペプチド (2010)
  • Inventor(s): 西森利數
  • Industrial Property Rights Holder: 西森利數
  • Filing Date: 2010-12-09
  • Overseas