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Search and characterization of new helicase inhibitors and others from coral reef organisms

Research Project

Project/Area Number 22603007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field ケミカルバイオロジー
Research InstitutionUniversity of the Ryukyus

Principal Investigator

TANAKA Junichi  琉球大学, 理学部, 教授 (20163529)

Co-Investigator(Renkei-kenkyūsha) NODA Naohiro  産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (70415727)
ANNO Kumiko  広島大学, 薬学部, 助教 (30452585)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsC 型肝炎ウイルス / NS3 ヘリケース / サンゴ礁生物 / 海綿 / ウミシダ / NS3ヘリケース / HCV / PBDE / C型肝炎ウイルス / psammaplin A / halenaquinol sulfate
Research Abstract

To discover lead molecules against hepatitis C virus, we screened extracts of coral reef organisms with PET assay targeting NS3 helicase and with replicon assay utilizing cultured hepatocarcinoma cells transfected with virus-like genes. Hit extracts were subjected to chemical characterization to identify or determine the bioactive molecules. In addition, we examined the mode of action of the hit molecules.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (24 results)

All 2013 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (17 results)

  • [Journal Article] Inhibition of hepatitis C virus NS3 helicase by cholesterol sulfate without inhibition of ATPase and RNA binding2013

    • Author(s)
      Yamashita, A.; Moriishi, K.; Tsubuki, M.; Nakakoshi, M.; Sekiguchi, Y.; Tsuneda, S.; Noda, N
    • Journal Title

      J. Enz. Inhib. Med. Chem

      Volume: 28 Issue: 2 Pages: 1-7

    • DOI

      10.3109/14756366.2013.766607

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Psammaplin A inhibits hepatitis C virus NS3 helicase2013

    • Author(s)
      Salam, K.A.; Furuta, A.; Noda, N.; Tsuneda, S.; Sekiguchi, Y.; Yamashita, A.; Moriishi, K.; Nakakoshi, M.; Tsubuki, M.; Tani, H.; Tanaka, J.; Akimitsu, N
    • Journal Title

      J. Nat. Med. in press

      Volume: 67 Issue: 4 Pages: 765-772

    • DOI

      10.1007/s11418-013-0742-7

    • NAID

      40019787969

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of Hepatitis C Virus Replication and Viral Helicase by Ethyl Acetate Extract of the Marine Feather Star Alloeocomatella polycladia2012

    • Author(s)
      Yamashita A, Maekawa S, et al
    • Journal Title

      Mar.Drugs

      Volume: 10 Issue: 4 Pages: 744-761

    • DOI

      10.3390/md10040744

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of both protease and helicase activities of hepatitis C virus NS3 by an ethyl acetate extract of marine sponge Amphimedon sp.2012

    • Author(s)
      Fujimoto Y
    • Journal Title

      PLoS One.

      Volume: 7 Issue: 11 Pages: 744-761

    • DOI

      10.1371/journal.pone.0048685

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of hepatitis C Virus NS3 helicase by manoalide2012

    • Author(s)
      Salam, K.A.
    • Journal Title

      J.Nat.Prod.

      Volume: (in press) Issue: 4 Pages: 650-654

    • DOI

      10.1021/np200883s

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of Hepatitis C Virus Replication and Viral Helicase by Ethyl Acetate Extract of the Marine Feather Star Alloeocomatella polycladia2012

    • Author(s)
      Yamashita, A., 他
    • Journal Title

      Marine Drugs

      Volume: 10 Pages: 744-761

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real-time monitoring of RNA helicase activity using fluorescence resonance energy transfer in vitro2010

    • Author(s)
      Tani, H.; Fujita, O.; Furuta, A.; Matsuda, Y.; Miyata, R.; Akimitsu, N.; Tanaka, J.; Tsuneda, S.; Sekiguchi, Y.; Noda, N.
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 393 Issue: 1 Pages: 131-136

    • DOI

      10.1016/j.bbrc.2010.01.100

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 海洋生物抽出物より取得した複数種の硫酸化合物による C 型肝炎ウイルス NS3 helicase 阻害作用2012

    • Author(s)
      古田篤史 他
    • Organizer
      海洋生物抽出物より取得した複数種の硫酸化合物による C 型肝炎ウイルス NS3 helicase 阻害作用
    • Place of Presentation
      神戸国際会議場.
    • Year and Date
      2012-10-25
    • Related Report
      2012 Final Research Report
  • [Presentation] Identification of novel hepatitis C virus NS3 inhibitors: psammaplin A and halisulfate 32012

    • Author(s)
      Salam, K.A.
    • Organizer
      Japan-China Medical Workshop 2012 on DrugDiscoveries and Therapeutics - New Perspectives in Cancer Prevention
    • Place of Presentation
      東京大学
    • Year and Date
      2012-10-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Inhibition of hepatitis C virus replication and viral helicase by ethyl acetate extract of the marine feather star Alloeocomatella polycladia2012

    • Author(s)
      Yamashita, A
    • Organizer
      25th International Conference on Antiviral Research
    • Place of Presentation
      Hotel Royton - Sapporo.
    • Year and Date
      2012-04-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Search for inhibitors against HCV NS3 helicase and chemical and genetic diversity of Sarcophyton soft corals2011

    • Author(s)
      Tanaka, J
    • Organizer
      VIIth US-Japan Seminar on Marine Natural Products: Cross-disciplinary Expansions in Marine Bioorganic Chemistry
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2011-12-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Search for inhibitors against HCV NS3 helicase and chemical and genetic diversity of Sarcophyton soft corals2011

    • Author(s)
      Tanaka, J.
    • Organizer
      VIIth U.S.-Japan Seminar on Marine Natural Products : Cross-disciplinary Expansions in Marine Bioorganic Chemistry
    • Place of Presentation
      沖縄コンベンションセンター(招待講演)
    • Year and Date
      2011-12-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] 沖縄産ウミシダAlloeocomatella polycladia 抽出物の抗HCV NS3 helicase阻害作用によるHCV増殖抑制効果2011

    • Author(s)
      山下篤哉 他
    • Organizer
      第58回日本ウイルス学会
    • Place of Presentation
      徳島県郷土文化会館
    • Year and Date
      2011-11-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 海洋生物抽出物より取得したcholesterol sulfate による C 型肝炎ウイルス NS3 helicase 阻害作用2011

    • Author(s)
      古田篤史他
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学.
    • Year and Date
      2011-09-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 海洋生物抽出物より取得したcholesterol sulfateによるC型肝炎ウイルスNS3 helicase阻害作用2011

    • Author(s)
      古田篤史, 他10名
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Inhibitors against NS3 helicase of hepatitis C virus from coral reef invertebrates2011

    • Author(s)
      Tanaka, J
    • Organizer
      2011 American Society of Pharmacognosy Meeting
    • Place of Presentation
      Paradise Point Resort, San Diego, USA
    • Year and Date
      2011-07-03
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] Identification and biochemical characterization of novel hepatitis C virus NS3 inhibitors2011

    • Author(s)
      Salam, K.A
    • Organizer
      manoalide and psammaplin A
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2011-06-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Identification and biochemical characterization of novel hepatitis C virus NS3 inhibitors, manoalide and psammaplin A2011

    • Author(s)
      Salam, K.A., 他11名
    • Organizer
      16th Annual Meeting of the RNA Society
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2011-06-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 海洋生物をライブラリーソースとした HCV NS3 Helicase 活性阻害化合物の探索2011

    • Author(s)
      山下篤哉他
    • Organizer
      第21回抗ウイルス療法研究会
    • Place of Presentation
      金沢市文化ホール
    • Year and Date
      2011-05-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 海洋生物をライブラリーソースとしたHCV NS3 Helicase活性阻害化合物の探索2011

    • Author(s)
      山下篤哉, 他17名
    • Organizer
      第21回抗ウイルス療法研究会
    • Place of Presentation
      金沢市文化ホール
    • Year and Date
      2011-05-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 沖縄産ウミシダAlloeocomatella polycladia抽出物の抗HCV NS3 helicase阻害活性によるHCV増殖抑制効果2010

    • Author(s)
      山下篤哉、古田篤史、松田泰嘉、谷英典、藤田統、秋光信佳、田中淳一、池田正徳、加藤宣之、前川信哉、榎本信幸、伊藤正彦、常田聡、関口勇地、野田尚宏
    • Organizer
      第58回日本ウイルス学会
    • Place of Presentation
      徳島県郷土文化会館、徳島
    • Year and Date
      2010-11-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of novel hepatitis C virus NS3 inhibitors: psammaplin A and halisulfate 3

    • Author(s)
      Salam, K. A. 他
    • Organizer
      Japan-China Medical Workshop 2012 on Drug Discoveries and Therapeutics - New Perspectives in Cancer Prevention, Diagnosis, Treatments, and Drug Discoveries
    • Place of Presentation
      東京大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 海洋生物抽出物より取得した複数種の硫酸化合物によるC型肝炎ウイルスNS3helicase阻害作用

    • Author(s)
      古田篤史 他
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] Inhibition of hepatitis C virus replication and viral helicase by ethyl acetate extract of the marine feather star Alloeocomatella polycladia

    • Author(s)
      Yamashita, A. 他
    • Organizer
      25th International Conference on Antiviral Research
    • Place of Presentation
      Hotel Royton - Sapporo
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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