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Fluorescence imaging analysis of cell-penetrating antimicrobial peptide with potentiated activity

Research Project

Project/Area Number 22603012
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field ケミカルバイオロジー
Research InstitutionTokyo University of Science

Principal Investigator

HASHIMOTO Shigeki  東京理科大学, 基礎工学部, 講師 (10287477)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords抗菌ペプチド / アピデシン / 抗菌活性 / グラム陽性菌 / グラム陰性菌 / 大腸菌 / アミノ酸 / 細胞膜 / 細胞膜透過 / 蛍光色素 / 作用機構
Research Abstract

Apidaecin is an antimicrobial peptide produced by honey bee. This peptide selectively inhibits the growth of Gram-negative bacteria such as Escherichia coli. In this study, the key amino acids in apidaecin sequence were substituted with other amino acids to improve the bacteriostatic activity and target selectivity of wild-type peptide. Interestingly, the resultant mutant peptides have shown potent activities for Gram-positive bacteria, which is originally insensitive to apidaecin. This finding has important implications for clarification of the action mechanism of apidaecin

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (7 results)

All 2012 2011 2010 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results)

  • [Journal Article] Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides : Case study on engineered apidaecins2010

    • Author(s)
      Ken'ichiro Matsumoto, Yoshitake Orikasa, Kenta Ichinohe, Shigeki Hashimoto, Toshihiko Ooi, Seiichi Taguchi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 395 Pages: 7-10

    • NAID

      120002148670

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 可変領域への変異導入とその組み合わせによる抗菌ペプチド「アピデシン」の抗菌スペクトル改変2012

    • Author(s)
      三宅政裕,岩村雄太,松本謙一郎,橋本茂樹,大井俊彦,田口精一
    • Organizer
      日本農芸化学会北海道支部平成24年度講演会
    • Place of Presentation
      北海道大学農学部
    • Year and Date
      2012-11-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 抗菌ペプチド「アピデシン」の可変領域への変異導入とその抗菌活性への影響2012

    • Author(s)
      三宅政裕,岩村雄太,松本謙一郎,橋本茂樹,大井俊彦,田口精一
    • Organizer
      日本農芸化学会年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-25
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] アミノ酸残基置換による抗菌ペプチド「アピデシン」の抗菌スペクトルの改変2011

    • Author(s)
      岩村雄太,山形享子,若林雅明,松本謙一郎,橋本茂樹,大井俊彦,田口精一
    • Organizer
      日本農芸化学会北海道支部学術講演会
    • Place of Presentation
      北海道大学農学部
    • Year and Date
      2011-11-04
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 抗菌ペプチド「アピデシン」のN末端領域分子デザインによる高活性変異体の作製と抗菌スペクトル評価2011

    • Author(s)
      若林雅明,岩村雄太,松本謙一郎,橋本茂樹,大井俊彦,田口精一
    • Organizer
      日本農芸化学会 2011年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2011-03-26
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] N末分子デザインによる抗菌ペプチド「アピデシン」の高活性体創出2010

    • Author(s)
      山形享子,橋本茂樹,折笠善丈,松本謙一郎,田口精一
    • Organizer
      第62回 日本生物工学会大会
    • Place of Presentation
      宮崎シーガイアワールドコンベンションセンターサミット
    • Year and Date
      2010-10-29
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] 可変領域への変異導入とその組み合わせによる抗菌ペプチド「アピデシン」の抗菌スペクトル改変

    • Author(s)
      三宅政裕,岩村雄太,松本謙一郎,橋本茂樹,大井俊彦,田口精一
    • Organizer
      日本農芸化学会北海道支部平成24年度講演会
    • Place of Presentation
      北海道大学農学部
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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