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Detection and observation of oxidative damage for the improvement of high-LET radiation therapy

Research Project

Project/Area Number 22611015
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical Physics/Radiological Technology
Research InstitutionTokai University

Principal Investigator

ITO Atsushi  東海大学, 工学部, 教授 (80193473)

Co-Investigator(Kenkyū-buntansha) ISHII Naoaki  東海大学, 医学部, 教授 (60096196)
HIRAYAMA Ryoichi  独立行政法人放射線医学総合研究所, 重粒子医科学センター, 研究員 (90435701)
Co-Investigator(Renkei-kenkyūsha) MURAYAMA Chieko  東海大学, 医学部, 講師 (50307295)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords高LET放射線 / 間接作用 / ラジカル / 重粒子 / DNA酸化損傷 / 免疫染色法 / 8-OHdG / 酸素効果 / トラック構造 / DNA薄膜 / クラスター損傷 / 蛍光抗体法
Research Abstract

In order to visualize the distribution of a penumbra, a low LET region around ion tracks, we have developed the methods to image the distribution of 8-OHdG, a radiation product resulting from the reaction of OH radicals with guanine residue, using an immunofluorescence staining for 1) mouse brain tissue, 2) human A549 cells, and 3) DNA thin film. Positive stains were evident particularly in the peripheral region of the cerebrum and in Purkinje cells of the cerebellum in mice. The immunofluorescence staining of cell nuclei was detected at a dose as small as 5 Gy where we can distinguish each incoming particle. DNA film with an insoluble property to water was developed with special reference to the uniformity, and the treatment with Fenton reaction to generate OH radicals resulted in the successful staining of uniformly formed 8-OHdG.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (37 results)

All 2013 2012 2011 2010 Other

All Journal Article (21 results) (of which Peer Reviewed: 13 results) Presentation (16 results) (of which Invited: 1 results)

  • [Journal Article] 重粒子線照射細胞におけるDNA損傷の修復の観察2013

    • Author(s)
      田向未来、小山裕二
    • Journal Title

      東海大学工学部エネルギー工学科卒業論文

    • Related Report
      2012 Final Research Report
  • [Journal Article] Mathematical models of the generation of radiation-induced DNA double-strand breaks2013

    • Author(s)
      Y. Saisho
    • Journal Title

      Journal of Mathematical Biology

      Volume: なし

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] athematical models of the generation of radiation-induced DNA double-strand breaks2012

    • Author(s)
      Y. Saisho and A. Ito
    • Journal Title

      J. Mathematical Biol

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Model animals for the study of oxidative stress from complex II2012

    • Author(s)
      T. Ishii, M. Miyazawa, H. Onouchi, K. Yasuda, P.S. Hartman, N. Ishii
    • Journal Title

      Biochim.Biophys. Acta

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oxidative stress induced inflammation initiates functional decline of tear production2012

    • Author(s)
      Y. Uchino, T. Kawakita, M. Miyazawa, T. Ishii, H. Onouchi, K. Yasuda, Y. Ogawa, S. Shimmura, N. Ishii, K. Tsubota
    • Journal Title

      PLoS ONE

      Volume: 7

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA 酸化損傷8-OHdGを指標とした高LET線の生物作用の解明2012

    • Author(s)
      伊藤敦、北畠里実、込宮大伍、平山亮一、石井直明、石井恭正、宮沢正樹、篠生環、村山千恵子、古澤佳也
    • Journal Title

      平成23年度放射線医学総合研究所重粒子線がん治療装置等共同利用研究報告書

      Pages: 126-127

    • Related Report
      2012 Final Research Report
  • [Journal Article] 重粒子線によるDNA酸化産物の計測~酸化損傷のLET依存性~2012

    • Author(s)
      北畠里実
    • Journal Title

      東海大学工学部エネルギー工学科卒業論文

    • Related Report
      2012 Final Research Report
  • [Journal Article] DNA酸化損傷8-OHdGを指標とした高LET線の生物作用の解明2012

    • Author(s)
      伊藤 敦
    • Journal Title

      平成23年度放射線医学総合研究所重粒子線がん治療装置等共同利用研究報告書

      Volume: なし Pages: 126-127

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Detection of DNA-protein crosslinks (DPCs) by novel direct fluorescence labeling methods: distinct stabilities of aldehyde and radiation-induced DPCs2012

    • Author(s)
      M. I. Shoulkamy
    • Journal Title

      Nucleic Acids Research

      Volume: 40

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Furusawa: Induction of DNA DSB and its rejoining in clamped and non-clamped tumors after exposure to carbon ion beams in comparison to X-rays2011

    • Author(s)
      R. Hirayama, A. Uzawa, Y. Matsumoto, M. Noguchi, Y. Kase, N. Takase, A. Ito, S. Koike, K. Ando, R. Okayasu, Y
    • Journal Title

      Radiat.Prot. Dosimetry

      Volume: 143 Pages: 508-512

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression associated with DNA-dependent protein kinase activity under normoxia, hypoxia, and reoxygenation2011

    • Author(s)
      T. Tsuchimoto, K. Sakata, M. Someya, H. Yamamoto, R. Hirayama, Y. Matsumoto, Y. Furusawa, M. Hareyama
    • Journal Title

      Journal of Radiation Research

      Volume: 52 Pages: 464-471

    • NAID

      10029122194

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial superoxide anion(O_・-) inducible "mev-1" animal models for aging research2011

    • Author(s)
      T. Ishii, M. Miyazawa, P.S. Hartman, N. Ishii
    • Journal Title

      BMB rep

      Volume: 44 Pages: 298-305

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial reactive oxygen species generation by the SDHC V69E mutation causes low birth weight and neonatal growth retardation2011

    • Author(s)
      T. Ishii, M Miyazawa, A. Onodera, K. Yasuda, N. Kawabe, M. Kirinashizawa, S. Yoshimura, N. Maruyama, P.S. Hartman, N. Ishii
    • Journal Title

      Mitochondrion

      Volume: 11 Pages: 155-165

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA 酸化損傷8-OHdGを指標とした高LET線の生物作用の解明2011

    • Author(s)
      伊藤敦、上野瑞己、秋山竜城、平山亮一、石井直明、石井恭正、宮沢正樹、篠生環、村山千恵子、古澤佳也
    • Journal Title

      平成23年度放射線医学総合研究所重粒子線がん治療装置等共同利用研究報告書

      Pages: 107-109

    • Related Report
      2012 Final Research Report
  • [Journal Article] 重粒子線照射によるマウス組織内でのDNA酸化産物の生成の観測・計測に関する実験2011

    • Author(s)
      上野瑞己
    • Journal Title

      東海大学大学院工学研究科応用理学専攻修士論文

    • Related Report
      2012 Final Research Report
  • [Journal Article] Induction of DNA DSB and its rejoining in clamped and non-clamped tumors after exposure to carbon ion beams in comparison to X-rays2011

    • Author(s)
      R.Hirayama, et al
    • Journal Title

      Radiation Protection Dosimetry

      Volume: 143 Pages: 508-512

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of wild-type p53 in apoptotic and non-apoptotic cell death induced by X-irradiation and heat treatment in p53-mutated mouse M10 cells2010

    • Author(s)
      A. Ito, H. Nakano, K. Shinohara
    • Journal Title

      J. Radiat. Res

      Volume: 51 Pages: 665-673

    • NAID

      10026964023

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Post-dauer life span of Caenorhabditis elegans dauer larvae can be modified by X-irradiation2010

    • Author(s)
      A. Onodera S. Yanase, T. Ishii, K. Yasuda, M. Miyazawa, P.S. Hartman N. Ishii
    • Journal Title

      J. Radiat. Res

      Volume: 51 Pages: 67-71

    • NAID

      10025914167

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct and indirect actions to high-LET radiations2010

    • Author(s)
      R. Hirayama, Y. Matsumoto, Y. Kase, M.Noguchi, N. Takase, A. Uzawa, A. Ito,N. Matsufuji, K. Ando, R. Okayasu, Y.Furusawa
    • Journal Title

      NIRS-KI Joint Symposium on Ion-Radiation Sciences

      Volume: 235 Pages: 155-162

    • Related Report
      2012 Final Research Report
  • [Journal Article] Role of wild type p53 in apoptotic and non-apoptotic cell death induced by X-irradiation and heat treatment in p53-mutated mouse M10 cells2010

    • Author(s)
      A.Ito, et al.
    • Journal Title

      Journal of Radiation Research

      Volume: 51 Pages: 665-673

    • NAID

      10026964023

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct and indirect actions to high-LET radiations2010

    • Author(s)
      R.Hirayama, et al.
    • Journal Title

      NIRS-KI Joint Symposium on Ion-Radiation Sciences, NIRS-M-235

      Pages: 155-162

    • Related Report
      2010 Annual Research Report
  • [Presentation] DNA2本鎖切断生成の確率モデル2012

    • Author(s)
      税所康正、伊藤敦
    • Organizer
      生物数学の理論とその応用
    • Place of Presentation
      京都大学数理解析研究所
    • Year and Date
      2012-11-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内8-OHdG検出による高LET領域での酸素効果減少機構の検証2012

    • Author(s)
      北畠里実、込宮大伍、秋山竜城、上野瑞己、伊藤敦、村山千恵子、平山亮一、古澤佳也
    • Organizer
      日本放射線影響学会第55回大会
    • Place of Presentation
      仙台
    • Year and Date
      2012-09-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 低LET放射線によるDNA2本鎖切断生成過程の確率モデル2012

    • Author(s)
      伊藤敦、税所康正
    • Organizer
      日本放射線影響学会第55回大会
    • Place of Presentation
      仙台
    • Year and Date
      2012-09-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 重粒子線によるDNA酸化産物の計測~酸化損傷のLET依存性~2012

    • Author(s)
      北畠里実, 他
    • Organizer
      日本原子力学会関東・甲越支部第5回学生研究発表会
    • Place of Presentation
      神奈川(湘南工科大)
    • Year and Date
      2012-03-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] 重粒子線の直接作用がもたらす生物効果2012

    • Author(s)
      平山亮一, 他
    • Organizer
      第14回癌治療増感研究シンポジウム
    • Place of Presentation
      奈良(猿沢荘)
    • Year and Date
      2012-02-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 酸素効果のメカニズムにおける化学的因子と生物学的因子2012

    • Author(s)
      平山亮一
    • Organizer
      日本放射線影響学会第55回大会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 低LET放射線によるDNA2本鎖切断生成過程の確率モデル2012

    • Author(s)
      伊藤敦
    • Organizer
      日本放射線影響学会第55回大会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 細胞内8-OHdG検出による高LET領域での酸素効果減少機構の検証2012

    • Author(s)
      北畠里実
    • Organizer
      日本放射線影響学会第55回大会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] DNA2本鎖切断生成の確率モデル2012

    • Author(s)
      税所康正
    • Organizer
      生物数学の理論とその応用
    • Place of Presentation
      京都大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] Oxygen enhancement ratio for different particles2011

    • Author(s)
      Ryoichi Hirayama
    • Organizer
      International Open Laboratory Radiation Quality Research Unit Kick-Off Meeting
    • Place of Presentation
      Darmstadt, Germany
    • Year and Date
      2011-09-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Direct action is the key to understand the high biological effects caused by particle beams2011

    • Author(s)
      Ryoichi Hirayama, et al
    • Organizer
      14th International Congress of Radiation Research
    • Place of Presentation
      Warsaw, Poland
    • Year and Date
      2011-08-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] トラック構造と生物効果2011

    • Author(s)
      平山亮一
    • Organizer
      平成23年度京都大学原子炉実験所専門研究会
    • Place of Presentation
      大阪(京大原子炉)
    • Year and Date
      2011-07-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] 重粒子線の放射線作用におる高RBEのメカニズム2010

    • Author(s)
      平山亮一, ら
    • Organizer
      日本放射線影響学会第53回大会
    • Place of Presentation
      京都テルサ(京都)
    • Year and Date
      2010-10-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] The type of DSB by direct action of X-rays is different comparison with the DSB induced by indirect action2010

    • Author(s)
      R.Hirayama, et al.
    • Organizer
      38th Annual Meeting of the European Radiation Research Society
    • Place of Presentation
      Stockholm, Sweden
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 水の放射線分解によって生じたフリーラジカルの生物影響

    • Author(s)
      平山亮一
    • Organizer
      平成24年度第1回量子ビーム科学研究施設研究会
    • Place of Presentation
      大阪大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Direct action of heavy-ion plays a major role in the high biological effects

    • Author(s)
      R. Hirayama
    • Organizer
      12th International Workshop on Radiation Damage to DNA
    • Place of Presentation
      Mariott Hotel, Prague (Czech Republic)
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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