Study of designer cellulosome for complete saccharification of soft-biomass
| Project/Area Number |
22612001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomass energy
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| Research Institution | Mie University |
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Principal Investigator |
MIYAKE Hideo 三重大学, 大学院・生物資源学研究科, 助教 (50362364)
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| Co-Investigator(Renkei-kenkyūsha) |
KURODA Kouichi 京都大学, 大学院・農学研究科, 准教授 (30432339)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | cellulosome / Clostridium cellulovoran / proteome / セルロソーム / バイオマス / セルラーゼ / Clostridium / プロテオーム / 相互作用 / セルロソーム骨格タンパク質 / Clostridium cellulovorans / CbpA / CbpB / キシラナーゼ / Clostridium acetobutylicum |
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| Research Abstract |
Proteome analysis of the Clostridium cellulovorans after culture in various carbon sources demonstrated to find enzymes which are necessary to reconstruct a cellulosome. Not only several carbohydrate enzymes but also proteins of unknown function were detected. Genomic DNA coding for scaffolding protein cbpB and 10 cellulosomal enzymes were detected by proteome analysis were subcloned in order to express and produce the recombinant protein to reconstruct a cellulosome.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Comparison of the mesophilic cellulosome-producing Clostridium cellulovorans genome with other cellulosome-related clostridial genomes2011
Author(s)
Tamaru, Y., Miyake, H., Kuroda, K., Nakanishi, A., Matsushima, C., Doi, R.H., Ueda, M
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Journal Title
Microbial Biotechnology
Volume: VOL4
Pages: 64-73
Related Report
Peer Reviewed
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