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Study of designer cellulosome for complete saccharification of soft-biomass

Research Project

Project/Area Number 22612001
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomass energy
Research InstitutionMie University

Principal Investigator

MIYAKE Hideo  三重大学, 大学院・生物資源学研究科, 助教 (50362364)

Co-Investigator(Renkei-kenkyūsha) KURODA Kouichi  京都大学, 大学院・農学研究科, 准教授 (30432339)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordscellulosome / Clostridium cellulovoran / proteome / セルロソーム / バイオマス / セルラーゼ / Clostridium / プロテオーム / 相互作用 / セルロソーム骨格タンパク質 / Clostridium cellulovorans / CbpA / CbpB / キシラナーゼ / Clostridium acetobutylicum
Research Abstract

Proteome analysis of the Clostridium cellulovorans after culture in various carbon sources demonstrated to find enzymes which are necessary to reconstruct a cellulosome. Not only several carbohydrate enzymes but also proteins of unknown function were detected. Genomic DNA coding for scaffolding protein cbpB and 10 cellulosomal enzymes were detected by proteome analysis were subcloned in order to express and produce the recombinant protein to reconstruct a cellulosome.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (40 results)

All 2013 2012 2011 2010 Other

All Journal Article (11 results) (of which Peer Reviewed: 8 results) Presentation (23 results) Book (5 results) Remarks (1 results)

  • [Journal Article] Display of Clostridium cellulovorans xylose isomeraseon the cell surface of Saccharomyces cerevisiae and its direct application to xylose fermentation2013

    • Author(s)
      Ota M., Sakuragi H., Morisaka H., Kuroda K., Miyake H., Tamaru Y,Ueda M
    • Journal Title

      Biotechnol. Prog

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources2012

    • Author(s)
      Morisaka H., Matsui K., Tatsukami Y., Kuroda K., Miyake H., Tamaru Y., Ueda M..
    • Journal Title

      AMB Expres

      Volume: 2 Pages: 37-42

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] セルロソーム生産菌Clostridium cellulovorans 743Bの全ゲノム解析2011

    • Author(s)
      三宅英雄、田丸浩
    • Journal Title

      三重大学大学院生物資源学研究科紀要

      Volume: 37 Pages: 1-9

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of the mesophilic cellulosome-producing Clostridium cellulovorans genome with other cellulosome-related clostridial genomes2011

    • Author(s)
      Tamaru Y., Miyake H., Kuroda K., Nakanishi A., Matsushima C., Doi R.H., Ueda M
    • Journal Title

      Microbial. Biotechnology

      Volume: 4 Pages: 64-73

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Putative role of cellulosomal protease inhibitors in Clostridium cellulovorans based on gene expression and measurement of activities2011

    • Author(s)
      Meguro H, Morisaka H, Kuroda K, Miyake H, Tamaru Y, Ueda M
    • Journal Title

      Journal of Bacteriology

      Volume: 193 Pages: 5527-5530

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] セルロソーム生産菌Clostridium cellulovorans 743Bの全ゲノム解析2011

    • Author(s)
      三宅英雄, 田丸浩
    • Journal Title

      三重大学大学院生物資源学研究科紀要

      Volume: 37巻 Pages: 1-9

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of the mesophilic cellulosome-producing Clostridium cellulovorans genome with other cellulosome-related clostridial genomes2011

    • Author(s)
      Tamaru, Y., Miyake, H., Kuroda, K., Nakanishi, A., Matsushima, C., Doi, R.H., Ueda, M
    • Journal Title

      Microbial Biotechnology

      Volume: VOL4 Pages: 64-73

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ソフトバイオマス完全糖化の新バイオ技術2010

    • Author(s)
      植田充美, 黒田浩一, 三宅英雄, 田丸浩
    • Journal Title

      配管技術(日本工業出版)

      Volume: 52巻 Pages: 1-6

    • Related Report
      2010 Annual Research Report
  • [Journal Article] バイオマスを完全分解・糖化する革新的微生物前処理技術に向けて2010

    • Author(s)
      植田充美, 黒田浩一, 三宅英雄, 田丸浩
    • Journal Title

      ブレインテクノニュース(農研)

      Volume: 139巻 Pages: 20-27

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Comparative genomics of the mesophilic cellulosome-producing Clostridium cellulovorans and its application to biofuel production via consolidated bioprocessing2010

    • Author(s)
      Tamaru, Y., Miyake, H., Kuroda, K., Ueda, M., Doi, R.H.
    • Journal Title

      Environmental Technology

      Volume: V0L31 Pages: 889-903

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ソフトバイオマス完全糖化を目指したデザイナブルセルロソームの構築2010

    • Author(s)
      三宅英雄, 田丸浩
    • Journal Title

      生物工学会誌

      Volume: 88巻 Pages: 336-339

    • NAID

      110007686920

    • Related Report
      2010 Annual Research Report
  • [Presentation] 異なる糖で培養したセルロソーム生産菌Clostridium cellulovorans 743Bの比較RNA-seq解析2013

    • Author(s)
      高嶋和也、石川卓、黒田浩一、植田充美、三宅英雄
    • Organizer
      日本農芸化学会 2012 年度大会
    • Place of Presentation
      東北大学
    • Year and Date
      2013-03-25
    • Related Report
      2012 Final Research Report
  • [Presentation] 異なる糖で培養したセルロソーム生産菌Clostridium cellulovorans 743Bの比較RNA-seq解析2013

    • Author(s)
      高嶋和也、石川卓、黒田浩一、植田充美、三宅英雄
    • Organizer
      日本農芸化学会 2012年度大会
    • Place of Presentation
      仙台
    • Related Report
      2012 Annual Research Report
  • [Presentation] バイオマス分解のためのClostridium cellulovorans の定量的セクレトーム解析2013

    • Author(s)
      松井一真, 江坂康平,森坂 裕信, 黒田浩一, 三宅 英雄, 田丸 浩,植田充美
    • Organizer
      日本農芸化学会 2012年度大会
    • Place of Presentation
      仙台
    • Related Report
      2012 Annual Research Report
  • [Presentation] Clostridium cellulovorans 743Bが生産する新規セルロソーム骨格タンパク質CbpBの機能解析2012

    • Author(s)
      三宅英雄、中島大地、永野彰彦、森坂裕信、黒田浩一、植田充美、田丸浩
    • Organizer
      第64回 日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2012-10-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Characterization of the cellulosomal scaffolding protein CbpB from Clostridium cellulovorans 743B2012

    • Author(s)
      MI YAKE H., NAKAJIMA D., NAGANO A., MORISAKA H., KURODA K., UEDA M., TAMARUY.
    • Organizer
      2012 Clostridium International Meeting XII
    • Place of Presentation
      Nottingham Conference Centre in Nottingham, UK
    • Year and Date
      2012-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Characterization of the cellulosomal scaffolding protein CbpB from Clostridium cellulovorans743B2012

    • Author(s)
      Nakajima D., Nagano A., Morisaka H., Kuroda K., Ueda M., Miyake H
    • Organizer
      American Society for Microbiology 17th
    • Place of Presentation
      Moscone Center in San Francisco, USA
    • Year and Date
      2012-06-17
    • Related Report
      2012 Final Research Report
  • [Presentation] Clostridium cellulovoransが生産するセルロソーム骨格タンパク質CbpBの機能解析2012

    • Author(s)
      中島大地、永野彰彦、森坂裕信、黒田浩一、植田充美、田丸浩、三宅英雄
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Clostridium cellulovoransによる種々の実バイオマスの分解とその評価2012

    • Author(s)
      川出雄二郎、三宅英雄、田丸浩
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Characterization of the cellulosomal scaffolding protein CbpB from Clostridium cellulovorans 743B2012

    • Author(s)
      Daichi Nakajima, Akihiko Nagano, Hironobu Morisaka, Kouichi Kuroda, Mitsuyoshi Ueda, Hideo Miyake
    • Organizer
      112th American Society for Microbiology
    • Place of Presentation
      アメリカ・サンフランシスコ
    • Related Report
      2012 Annual Research Report
  • [Presentation] Focused proteome analysis of cellulosome of Clostridium cellulovorans2012

    • Author(s)
      Kazuma Matsui, Hironobu Morisaka, Kouichi Kuroda, Yutaka Tamaru, Hideo Miyake, Mitsuyoshi Ueda
    • Organizer
      15th European Congress on Biotechnology
    • Place of Presentation
      トルコ・イスタンブール
    • Related Report
      2012 Annual Research Report
  • [Presentation] Yeast cell surface display of xylose isomerase from C. cellulovorans toward efficient xylose fermentation2012

    • Author(s)
      Kouichi Kuroda, Miki Ota, Hironobu Morisaka, Hideo Miyake, Yutaka Tamaru, Mitsuyoshi Ueda
    • Organizer
      15th European Congress on Biotechnology
    • Place of Presentation
      トルコ・イスタンブール
    • Related Report
      2012 Annual Research Report
  • [Presentation] Characterization of the cellulosomal scaffolding protein CbpB from Clostridium cellulovorans 743B2012

    • Author(s)
      Hideo Miyake, Daich Nakajima, Akihiko Nagano, Hironobu Morisaka, Kouichi Kuroda, Mitsuyoshi Ueda, Yutaka Tamaru
    • Organizer
      2012 Clostridium International Meeting XII
    • Place of Presentation
      イギリス・ノッティンガム
    • Related Report
      2012 Annual Research Report
  • [Presentation] Clostridium cellulovorans 743Bが生産する新規セルロソーム骨格タンパク質CbpBの機能解析2012

    • Author(s)
      三宅英雄、中島大地、永野彰彦、森坂裕信、黒田浩一、植田充美、田丸浩
    • Organizer
      第64回 日本生物工学会大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] Clostridium cellulovorans由来セルロソーム骨格タンパク質CbpBのクローニングと発現2011

    • Author(s)
      三宅英雄、中島大地、山本康介、森坂裕信、黒田浩一、植田充美、田丸浩
    • Organizer
      第34回 日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Clostridium cellulovorans由来セルロソーム骨格タンパク質CbpBのクローニングと発現2011

    • Author(s)
      三宅英雄、中島大地、山本康介、森坂裕信、黒田浩一、植田充美、田丸浩
    • Organizer
      日本分子生物学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] セルロソーム生産嫌気性細菌Clostridium cellulovoransの比較ゲノム解析2011

    • Author(s)
      山本康介、三宅英雄、黒田浩一、植田充美、田丸浩
    • Organizer
      日本分子生物学会 大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Clostridium cellulovorans のセルロソームに焦点を当てたプロテオーム解析2011

    • Author(s)
      松井一真、森坂裕信、黒田浩一、三宅英雄、田丸浩、植田充美
    • Organizer
      第63回 日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Clostridium cellulovoransのセルロソームに焦点を当てたプロテオーム解析2011

    • Author(s)
      松井一真、森坂裕信、黒田浩一、三宅英雄、田丸浩、植田充美
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Clostridium cellulovorans 743B由来セルロソーム骨格タンパク質CbpB遺伝子のクローニングと発現2011

    • Author(s)
      中島大地、山本康介、森坂裕信、黒田浩一、植田充美、田丸浩、三宅英雄
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cloning and protein expression of the cellulosomal scaffolding protein Cbpb from Clostridium cellulovorans 743b2011

    • Author(s)
      H.Miyake, D.Nakajima, K.Yamamoto, H.Morisaka, K.Kuroda, M.Ueda, Y.Tamaru
    • Organizer
      111th General Meeting American Society for Microbiology
    • Place of Presentation
      米国ニューオリンズ、コンベンションセンター
    • Year and Date
      2011-05-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Clostridium cellulovorans全ゲノム解析と糖質関連酵素遺伝子の全容解明2010

    • Author(s)
      田丸浩、三宅英雄、黒田浩一、Roy DOI、植田充美
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      シーガイア(宮崎県)
    • Year and Date
      2010-10-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Comparative Genomics of Clostridumu Cellulovorans with other Clostridia2010

    • Author(s)
      Miyake, Y.Tamaru, K.Kuroda, R.H.Doi, M.Ueda
    • Organizer
      General Meeting of American Society For Microbiology
    • Place of Presentation
      サンディエゴコンベンションセンター(アメリカ)
    • Year and Date
      2010-05-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Genome Sequence of the Cellulosome-producing Mesophilic Organism Clostridium cellulovorans 743B2010

    • Author(s)
      Y.Tamaru, H.Miyake, K.Kuroda, R.H.Doi, M.Ueda
    • Organizer
      General Meeting of American Society For Microbiology
    • Place of Presentation
      サンディエゴコンベンションセンター(アメリカ)
    • Year and Date
      2010-05-25
    • Related Report
      2010 Annual Research Report
  • [Book] バイオマス分解酵素研究の最前線 セルラーゼ・ヘミセルラーゼを中心として2012

    • Author(s)
      三宅英雄、田丸浩
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Final Research Report
  • [Book] バイオマス分解酵素研究の最前線2012

    • Author(s)
      三宅英雄、田丸浩
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Annual Research Report
  • [Book] デザイナブルセルロソームによるバイオマス処理技術" 「セルロース系バイオエタノール製造技術-食料クライシス回避のために- 第11章2010

    • Author(s)
      田丸浩, 三宅英雄
    • Publisher
      NTS
    • Related Report
      2012 Final Research Report
  • [Book] セルロース系バイオマスからのブタノール生産"「エコバイオリファイナリー」2010

    • Author(s)
      三宅英雄
    • Publisher
      シーエムシー
    • Related Report
      2012 Final Research Report
  • [Book] "セルロース系バイオマスからのブタノール生産"「エコバイオリファイナリー」2010

    • Author(s)
      三宅英雄
    • Publisher
      シーエムシー出版
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.bio.mie-u.ac.jp/~miyake/miyakes_HP/Home.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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