Functional analysis of a mechanical stress responsible geneFbxo32 on bone volume regulation

• Project/Area Number: 22616006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Mechanobiology
• Research Institution: Nagasaki University
• Principal Investigator: IZUMI Shinichi 長崎大学, 大学院・医歯薬学総合研究科, 助教 (40264246)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Carolina Andrea 長崎大学, 大学院・医歯薬学総合研究科, 助教 (50437828) ; MORIISHI Takeshi 長崎大学, 大学院・医歯薬学総合研究科, 技術職員 (20380983)
• Co-Investigator(Renkei-kenkyūsha): KOMORI Toshihisa 長崎大学, 大学院・医歯薬学総合研究科, 教授 (00252677) ; ITO Masako 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (10193517) ; FUKUYAMA Ryo 広島国際大学, 薬学部, 助教 (20389117)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: メカニカルストレス / 遺伝子 / 骨量 / 細胞 / 組織 / 発生・分化 / Fbxo / Runx2 / 細胞・組織 / ノックアウト

Research Abstract

We clarified a part of molecular mechanisms on bone volume regulation by analyzingFbxo32 knockout mice. By bone morphometric analysis after mouse tail suspensionexperiments, it appeared that Fbxo32 gene expression in osteoblasts was associated with the bone volume but not bone density in the bone formation. Further, wild type mice and Fbxo32 knockout mice were administered isoproterenol, similar morphometric value between the femurs was recognized, indicating that Fbxo32 was not involved in a sympathetic nerve-stimulated decrease of bone volume.

Research Products

• [Journal Article] es bone loss at unloading by promoting osteoclastogenesis (2012)
  • Author(s): Wang Y, Liu W, Masuyama R, Fukuyama R, Ito M, Zhang Q, Komori H, Murakami T, Moriishi T, Miyazaki T, Kitazawa R, Yoshida CA, Kawai Y, Izumi S, and Komori T
  • Journal Title: Bone Volume: 50 Pages: 409-419
  • Peer Reviewed
• [Journal Article] Pyruvate dehydrogenase kinase 4 induces bone loss at unloading by promoting (2012)
  • Author(s): Wang Y,Shinichi,I et al.
  • Journal Title: Bone Volume: 50 Pages: 409-419
  • Peer Reviewed
• [Journal Article] Pyruvate dehydrogenase kinase 4 induces bone loss at unloading by promoting osteoclastogenesis (2012)
  • Author(s): Wang Y, et al
  • Journal Title: Bone Volume: 50 Pages: 409-419
  • Peer Reviewed
• [Journal Article] Early onset of Runx2 expression causes craniosynostosis, ectopic bone formation, and limb defects (2011)
  • Author(s): Maeno T, Moriishi T, Yoshida C, Komori H, Kanatani N, Izumi S, Takaoka K, and Komori T
  • Journal Title: Bone Volume: 49 Pages: 673-682
  • Peer Reviewed
• [Journal Article] Regulation of Tcf7 by Runx2 in chondrocyte maturation and proliferation. (2010)
  • Author(s): Mikasa M, et al.
  • Journal Title: J Bone Miner.Metab. Volume: (Epub)
  • Peer Reviewed
• [Journal Article] Ultrastructural localization of Upf2 and its binding to mRNA molecules in human cells. (2010)
  • Author(s): Ishigaki Y, et al.
  • Journal Title: J Electr Microsc Technol Med Biol. Volume: 24(1) Pages: 8-13
• [Presentation] mRNAの分子構造可視化と結合タンパク質の機能解析 (2011)
  • Author(s): 石垣靖人, 他
  • Organizer: 医学生物学電子顕微鏡技術学会第27回学術講演会
  • Place of Presentation: 四国大学交流プラザ(徳島市)
  • Year and Date: 2011-05-14
• [Presentation] 骨芽細胞分化における抗アポトーシス分子Bcl-2の役割 (2010)
  • Author(s): 森石武史、和泉伸一、小守壽文
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-07-22
• [Presentation] 骨芽細胞分化における抗アポトーシス分子Bc1-2の役割 (2010)
  • Author(s): 森石武史, 他
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-07-22
• [Presentation] イオン液体の免疫電顕試料への帯電防止効果の検討 (2010)
  • Author(s): 和泉伸一, 他
  • Organizer: 医学生物学電子顕微鏡技術学会第26回学術講演会
  • Place of Presentation: 別府ビーコンプラザ(別府市)
  • Year and Date: 2010-05-16