Project/Area Number |
22616007
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Mechanobiology
|
Research Institution | Iwate Medical University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
SAITO Maki 岩手医科大学, 薬学部, 助教 (40365185)
鎌滝 章央 岩手医科大学, 医学部, 助教 (60360004)
|
Co-Investigator(Renkei-kenkyūsha) |
KAMATAKI Akihisa 岩手医科大学, 医学部, 助教 (60360004)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | メカニカルストレス / メタボリックシンドローム / 脂肪細胞 / マクロファージ / PPAR-γ / サイトカイン / 肺高血圧 / ホスホリパーゼA2 / 脂肪組織 / シクロオキシゲナーゼ / sPLA2 / アディポサイトカイン / 肺高血圧症 / アポトーシス |
Research Abstract |
The effects of abdominal vibration were examined using obese mice with high-fat diet. Daily exposure of the 30-minutes-vibration induced downregulation of adipogenic factors and upregulation of anti-adipogenic factor, which consisted with decreasing adipose tissue triglyceride, some adipokines, and plasma free fatty acids. Furthermore, augmented expressions of several genes characteristic of anti-inflammatory M2 macrophages (Mφ) were detected. The effects of selective sPLA2 inhibitor (indoxam) on the pathogenicity of MCT-induced fatal pulmonary hypertensive rats (MCT-PHR) were also examined. Indoxam abolished the enhanced contractilities of the pulmonary arteries of MCT-PHR, which arteries show augmented expression of sPLA2-X isoform. In addition, administration of indoxam attenuated some inflammatory signs such as expression of proinflammatory cytokines and apoptosis in the lung, leading to a significant improvement of survival ratio in MCT-PHR.
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