Stretch of muscle sarcomere induces protrusion of myosinheads toward actin so as to facilitate formation of contractile cross-bridges.

• Project/Area Number: 22616008
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Mechanobiology
• Research Institution: Jikei University School of Medicine
• Principal Investigator: TAKEMORI Shigeru 東京慈恵会医科大学, 医学部, 教授 (20179675)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Maki 東京慈恵会医科大学, 医学部, 講師 (30271315) ; KIMURA Masako 女子栄養大学, 栄養学部, 准教授 (30328314)
• Co-Investigator(Renkei-kenkyūsha): OHNO Tetsuo 東京慈恵会医科大学, 医学部, 助教 (30233224)
• Research Collaborator: NAKAHARA Naoya 東京慈恵会医科大学, 医学部, 助教 (10632193)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 横紋筋 / エックス線回折 / 収縮 / 筋節 / X線回折 / スキンドファイバー / 骨格筋 / 心筋 / ゲルゾリン / ミオシン / サルコメア / アクチン / Spring8 / ブタンジオンモノオキシム

Research Abstract

Stretch of sarcomeres of striated muscle induces higher contractile activity in striated muscle. Well organized fine structure of sarcomere is responsible to this stretch activation phenomenon, but its detailed mechanism has not been elucidated. Developing two-dimensional x-ray diffraction techniques, we found that stretch induces protrusion of myosin heads out of the thick filament backbone toward the neighboring actin molecules on thin filament so as to facilitate formation of contractile cross-bridges between myosin heads and actin molecules.

Research Products

• [Journal Article] Structure and function of skeletal muscle and locomotive systems: Involvement of water-state transitions (2012)
  • Author(s): Takemori S, Kimura M
  • Journal Title: J. Physical Fitness Sport Med Volume: 1 Pages: 95-101
  • NAID: 10031158930
  • Peer Reviewed
• [Journal Article] Structure and function of skeletal muscle and locomotive systems : Involvement of water-state transitions (2012)
  • Author(s): Takemori S, Kimura M
  • Journal Title: J Phys Fitness Sports Med Volume: 1(in press)
  • NAID: 10031158930
  • Peer Reviewed
• [Presentation] 横紋筋サルコメアのフィラメント格子の安定性 (2013)
  • Author(s): 竹森 重
  • Organizer: 日本生物物理学会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] (2012)
  • Author(s): Takemori S, Kimura M, Yamaguchi M, Ohno T, Nakahara N, Yokomizo S
  • Organizer: 日本生物物理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-23
• [Presentation] Multidemensional approaches to the secrets of life (2012)
  • Author(s): Takemori S
  • Organizer: 日本生物物理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-22
• [Presentation] 骨格筋の筋節伸展がミオシン頭部の固有の状態に及ぼす効果 (2012)
  • Author(s): 竹森重、山口眞紀、木村雅子、大野哲生, 他
  • Organizer: 第29回PFシンポジウム
  • Place of Presentation: 筑波
  • Year and Date: 2012-03-15
• [Presentation] 細いフィラメント除去筋線維内でのミオシン頭部の振る舞い (2011)
  • Author(s): 山口眞紀, ほか
  • Organizer: 日本生理・解剖学会合同大会
  • Place of Presentation: J.Physiol.Sci 誌上
  • Year and Date: 2011-03-29
• [Presentation] 細いフィラメント除去筋線維内でのミオシン頭部の振る舞い (2011)
  • Author(s): 山口眞紀,竹森 重,木村雅子,大野哲生,中原直哉,八木直人
  • Organizer: 日本生理学会大会・日本解剖学会総会合同大会
  • Place of Presentation: 紙上開催