Gene transfer techniques for study of songbird brain circuit

• Project/Area Number: 22650064
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Kyoto University
• Principal Investigator: WATANABE Dai 京都大学, 大学院・生命科学研究科, 教授 (90303817)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,330,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: ソングバード / トランスジェニック動物

Research Abstract

Avian species have provided an attractive model system to explore the development and function of brain circuits. Electroporation-mediated and retrovirus(including lentivirus) vector-mediated gene transfer techniques have so far widely been used for introducing genetic material into avian cells. However, it is still challenging to efficiently transduce avian postmitotic neurons without harming the cells. To overcome this problem, we searched a virus vector suitable for gene transfer into avian neurons, and characterize a novel recombinant virus vector, avian adeno-associated virus(A3V) vector.

Research Products

• [Journal Article] Compensation of depleted neuronal subsets by new neurons in a local area of the adult olfactory bulb (2011)
  • Author(s): Koshi Murata, Maki Imai, Shigetada Nakanishi, Dai Watanabe, Ira Pastan, Kazuto Kobayashi, Tomoko Nihira, Hideki Mochizuki, Shuichi Yamada, Kensaku Mori, Masahiro Yamaguchi
  • Journal Title: The Journal of Neuroscience Volume: 31 Issue: 29 Pages: 10540-10557
  • DOI: 10.1523/jneurosci.1285-11.2011
  • Peer Reviewed
• [Journal Article] Neural coding of syntactic structure in learned vocalizations in the songbird (2011)
  • Author(s): Fujimoto H, Hasegawa T, Watanabe D
  • Journal Title: J Neurosci Volume: 31 Issue: 27 Pages: 10023-10033
  • DOI: 10.1523/jneurosci.1606-11.2011
  • NAID: 120003203922
  • Peer Reviewed
• [Journal Article] miR-124a is required for hippocampal axogenesis and retinal cone survival through Lhx2 suppression (2011)
  • Author(s): Sanuki R, Onishi A, Koike C, Muramatsu R, Watanabe S, Muranishi Y, Irie S, Uneo S, Koyasu T, Matsui R, Cherasse Y, Urade Y, Watanabe D, KondoM, Yamashita T, Furukawa T
  • Journal Title: Nat Neurosci Volume: 14 Issue: 9 Pages: 1125-1134
  • DOI: 10.1038/nn.2897
  • Peer Reviewed
• [Journal Article] Songbirds possess the spontaneous ability to discriminate syntactic rules (2011)
  • Author(s): Abe K, Watanabe D
  • Journal Title: Nat Neurosci Volume: 14 Issue: 8 Pages: 1067-1074
  • DOI: 10.1038/nn.2869
  • Peer Reviewed
• [Journal Article] Selective optical control of synaptic transmission in the subcorticalvisual pathway by activation of viral vector-expressed halorhodopsin (2011)
  • Author(s): Kaneda K, Kasahara H, Matsui R, Katoh T, Mizukami H, Ozawa K, Watanabe D, Isa T
  • Journal Title: PLoSONE Volume: 6 Issue: 4 Pages: 18452-18452
  • DOI: 10.1371/journal.pone.0018452
  • Peer Reviewed
• [Presentation] 脳の情報処理を1細胞の精度で調べる (2012)
  • Author(s): Watanabe D.
  • Organizer: 第4回脳プロ公開シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2012-02-04
• [Presentation] A spontaneous ability of songbirds to discriminate syntactic rules in auditory information (2011)
  • Author(s): 安部健太郎
  • Organizer: Neuroscience 2011
  • Place of Presentation: The Walter E.Washington Convention Center (Washington, DC)
  • Year and Date: 2011-11-14
• [Presentation] Neural coding of syntax in learnedvocalizations in the songbird (2011)
  • Author(s): Watanabe D., Fujimoto H.
  • Organizer: Neuroscience 2011
  • Place of Presentation: Washington D. C.
  • Year and Date: 2011-11-13
• [Presentation] Neural coding of syntax in learned vocalizations in the songbird (2011)
  • Author(s): 渡邉大、藤本久貴
  • Organizer: Neuroscience 2011
  • Place of Presentation: The Walter E.Washington Convention Center (Washington, DC)
  • Year and Date: 2011-11-13
• [Presentation] Study of auditory and vocal behaviors in Bengalese finch-An approach for understanding the neural basis of syntactic computation in learned vocal communication- (2011)
  • Author(s): 渡邉大
  • Organizer: 第7回動物音響談話会
  • Place of Presentation: 同志社大学医心館(京田辺市)(招待講演)
  • Year and Date: 2011-10-15
• [Presentation] 鳥アデノ随伴ウイルスベクターを用いた孵化前及び孵化後のニワトリ脳への遺伝子導入 (2011)
  • Author(s): 松井亮介、渡邉大
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2011-09-23
• [Presentation] Approach to neural circuit mechanism underlying natural learned behavior (2011)
  • Author(s): Watanabe D.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-17
• [Presentation] Disturbance of dexterous hand movement of macaque monkeys induced by the pathway-specific blockade of the signal transmission through the mid-cervical propriospinal neurons (2011)
  • Author(s): Kinoshita M., Matsui R., Kato S., Kasahara H., Isa K., Nishimura Y., Watanabe D., Kobayashi K., Isa T
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-17
• [Presentation] Efficient gene transfer into the chick brain using avian adeno-associated virus vector (2011)
  • Author(s): Matsui R., Watanabe D.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-17
• [Presentation] 自然に獲得される行動の神経回路メカニズムへのアプローチ (2011)
  • Author(s): 渡邉大
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-09-17
• [Presentation] 鳥アデノ随伴ウイルスによる鶏脳への効率的な遺伝子導入 (2011)
  • Author(s): 松井亮介、渡邉大
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-09-17
• [Presentation] Neural coding of syntactic structure in learned vocalizations in songbirds (2011)
  • Author(s): Fujimoto H., Watanabe D.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-15
• [Presentation] 鳴禽類の高次音声情報処理能力とその発達過程 (2011)
  • Author(s): 安部健太郎、渡邉大
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-09-15
• [Presentation] 鳴禽類におけるシンタックス構造の神経内コード様式 (2011)
  • Author(s): 藤本久貴、渡邉大
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-09-15
• [Presentation] Optogenetically induced suppression of neural activity in the macaque primary motor cortex (2010)
  • Author(s): 木下正治、金田勝幸、笠原洋起、畑中伸彦、松井亮介、知見聡美、伊佐かおる、水上浩明、小澤敬也、渡辺大、南部篤、伊佐正
  • Organizer: 北米神経科学年会
  • Place of Presentation: San Diego
• [Presentation] Pathway specific optical inhibition of retino-collicular transmission by halorhodopsin expressed with viral vectors in mice (2010)
  • Author(s): 金田勝幸、笠原洋紀、松井亮介、加藤智子、水上浩明、小澤敬也、渡邉大、伊佐正
  • Organizer: 北米神経科学年会
  • Place of Presentation: San Diego
• [Presentation] マカクザル第一次運動野ニューロン活動の光遺伝学的抑制 (2010)
  • Author(s): 木下正治、金田勝幸、笠原洋起、畑中伸彦、松井亮介、知見聡美、伊佐かおる、水上浩明、小澤敬也、渡辺大、南部篤、伊佐正
  • Organizer: Neuro2010
  • Place of Presentation: 神戸
• [Presentation] ウイルスベクターを介したハロロドプシンの発現によるマウス網膜上丘シナプス伝達の経路特異的抑制 (2010)
  • Author(s): 金田勝幸、笠原洋紀、松井亮介、加藤智子、水上浩明、小澤敬也、渡邉大、伊佐正
  • Organizer: Neuro2010
  • Place of Presentation: 神戸
• [Presentation] Expression of halorhodopsin for suppression of neuronal transmission in the central nervous system of the mouse and monkey with viral vectors (2010)
  • Author(s): 木下正治、金田勝幸、笠原洋起、畑中伸彦、松井亮介、知見聡美、伊佐かおる、水上浩明、小澤敬也、渡辺大、南部篤、伊佐正
  • Organizer: 日本遺伝子治療学会
  • Place of Presentation: 栃木
• [Remarks]
  • URL: http://www.phy.med.kyoto-u.ac.jp/