Analysis of molecular dynamics in neuronal dendrites by simultaneous multi-point fluorescence correlation spectroscopy
| Project/Area Number |
22650066
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | 分子 / 細胞神経科学 / 蛍光相関分光法 / タンパク質 / 拡散 / 樹状突起 / スパイン / 2光子励起顕微鏡 / 分子・細胞神経科学 |
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| Research Abstract |
This research project aimed to measure dynamics of protein molecules rapidly moving in cytosole of neuronal dendrites at multiple points simultaneously by means of a newly developed two-photon microscope. I succeeded in recording at multiple points at more than 10 kHz time of resolution from cytosol of primary cultured neurons derived from mouse, in which GFP or GFP-fused CamKII protein was expressed, and in analyzing by FCS.
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Report
(4 results)
Research Products
(12 results)
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[Presentation] Illuminating passive permeability barrier of primary cilia using novel diffusion trap technique2013
Author(s)
Takanari Inoue, Yu-Chun Lin, Benjamin Lin, Siew Cheng Phua, John Jiao, Andre Levchenko, Pawel Niewiadomski, Rajat Rohatgi, Hideki Nakamura, Takafumi Inoue
Organizer
57th Annual Meeting of Biophysical Society
Place of Presentation
Philadelphia, February
Related Report
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