Analysis of a gene function using ZFN and recombineering
| Project/Area Number |
22650079
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Kyorin University (2011)
Tokyo Metropolitan Organization for Medical Research (2010) |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,330,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | ZFN / recombineering / CaMKII / dNZF / 遺伝子機能法 / コンディショナル・ノックアウト / C2HC型Znフィンガー / ショウジョウバエ |
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| Research Abstract |
A method for studying a gene function with complete control has been tried to develop by combinatorial use of a'zinc finger nuclease'(ZFN), which is a designed, sequence-specific endonuclease that can be customized to cleave a user-chosen DNA target, and recombineering, which is able to engineer large segments of genomic DNA of P[acman] for transgenesis in Drosophila melanogaster.
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Report
(3 results)
Research Products
(3 results)
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[Journal Article] Intracapsular Organization of Ciliary Zonules in Monkey Eyes.2010
Author(s)
Hiraoka, M., Inoue, K., Ohtaka-Maruyama, T., Ohsako, S., Kojima, N., Senoo, H., Takada, M.
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Journal Title
The Anatomical Record
Volume: 293
Pages: 1797-1804
Related Report
Peer Reviewed
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