| Project/Area Number |
22650079
|
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurochemistry/Neuropharmacology
|
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| Research Institution | Kyorin University (2011)
Tokyo Metropolitan Organization for Medical Research (2010) |
|---|
Principal Investigator |
|
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,330,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | ZFN / recombineering / CaMKII / dNZF / 遺伝子機能法 / コンディショナル・ノックアウト / C2HC型Znフィンガー / ショウジョウバエ |
|---|
| Research Abstract |
A method for studying a gene function with complete control has been tried to develop by combinatorial use of a'zinc finger nuclease'(ZFN), which is a designed, sequence-specific endonuclease that can be customized to cleave a user-chosen DNA target, and recombineering, which is able to engineer large segments of genomic DNA of P[acman] for transgenesis in Drosophila melanogaster.
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