Preparation of synaptosome derived from specified neural circuit
| Project/Area Number |
22650081
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Tottori University |
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Principal Investigator |
HATA Yoshio 鳥取大学, 大学院・医学系研究科, 教授 (40212146)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,320,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | 神経発生 / 神経発達 / 神経再生 / 神経再建 / 神経科学 / 脳・神経 / 神経回路 / シナプス |
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| Research Abstract |
The cerebral cortex consists of various types of neural cells which are interconnected and make up a complex network. Therefore, it is necessary to examine the behavior of functional molecules at specified neural connections to understand the function, development and plasticity of the cerebral cortex. The aim of the present project is to prepare the synaptosome sample derived from the neural connections that was fluorescently labeled in advance. To this end, I labeled the neurons in the visual cortex by injecting fluorescent tracers, prepared synaptosome samples from the labeled tissue, and sorted them using flowcytometry according to their fluorescent intensity. I could obtained the successfully labeled synaptosomes from cortical tissue injected with a carbocyanine dye DiI.
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Report
(3 results)
Research Products
(2 results)
