| Project/Area Number |
22650083
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Fusional basic brain science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KITAMURA Kazuo 東京大学, 大学院・医学系研究科, 准教授 (60423159)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,330,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | ニューロン / シナプス / 2光子イメージング / シナプス統合 / 樹状突起 / 体性感覚 |
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| Research Abstract |
We performed simultaneous whole-cell patch-clamp recordings and two-photon calcium imaging in layer 2/3 of mouse barrel cortex in vivo. Calcium signals in individual spines of layer 2/3 pyramidal cells induced by spontaneous and sensory-evoked activities could be clearly observed. We found that approximately 80% of synaptic inputs were localized only in 25% of total spines. Furthermore, we found that nearby spines were tended to be activated simultaneously. Thus, our results suggest that functionally coupled neurons innervate to nearby spines in dendrites of postsynaptic pyramidal neurons.
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