Exploring Optical Navigation of Neurite Outgrowth

• Project/Area Number: 22650084
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Fusional basic brain science
• Research Institution: Yokohama City University
• Principal Investigator: NAKAMURA Fumio 横浜市立大学, 医学部, 准教授 (10262023)
• Co-Investigator(Renkei-kenkyūsha): ISEKI Mineo 東邦大学, 薬学部, 准教授 (60414009)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,870,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 光脳科学 / 神経突起 / 神経再生 / 光受容体 / チロシンキナーゼ / Trk / MAPキナーゼ / 神経 / 突起伸長 / フォトトロピン / LOV領域 / Src

Research Abstract

To explore the optical navigation of neurite outgrowth, I utilized two photoreceptors : bacterial photoactivated adenylyl cyclase(bPAC) and an anonymous photoreceptor X. Transient expression of bPAC in cultured cells showed A-kinase activation upon light stimulation. I designed a fusion protein X-Trk, X-photoreceptor connected with the kinase domain of TrkB. Photostimulation upon the cells expressing X-Trk induced autophosphorylation of X-Trk and MAP kinase activation. Ectopic expression of X-Trk in chick embryonic dorsal root ganglion neurons facilitated the neurite outgrowth upon light stimulation. Enabling the optical navigation of neurite outgrowth with X-Trk may open new avenue to regenerate and analyze neural circuits.

Research Products

• [Journal Article] Decreased Expression of Semaphorin-3A, a Neurite-Collapsing Factor, is Associated With Itch in PsoriaticSkin. (2012)
  • Author(s): Kou K, Nakamura F,Aihara M, Chen H, Seto K, Komori-Yamaguchi J, Kambara T, Nagashima Y,Goshima Y, Ikezawa Z
  • Journal Title: Acta Derm Venereol Volume: 92 Issue: 5 Pages: 521-528
  • DOI: 10.2340/00015555-1350
  • Peer Reviewed
• [Journal Article] Phosphorylation of CRMP2 (Collapsin Response Mediator Protein 2) Is Involved in Proper Dendritic Field Organization (2012)
  • Author(s): Yamashita N, Ohshima T, Nakamura F, Kolattukudy P, Honnorat J, Mikoshiba K, Goshima Y
  • Journal Title: J Neurosci Volume: 32 Pages: 1360-1365
  • Peer Reviewed
• [Journal Article] Cartilage acidic protein-1B (LOTUS), an endogenous Nogo receptor antagonist for axon tract formation (2012)
  • Author(s): Sato Y, Iketani M, Kurihara K, Yamaguchi M, Yamashita N, Nakamura F, Aire Y, Kawasaki T, Hirata T, Abe T, Kiyonari H, Strittmatter SM, Goshima Y, Takei T
  • Journal Title: Science Volume: 333 Pages: 769-773
  • Peer Reviewed
• [Journal Article] GSK3β/Axin-1/β-catenin Complex is Involved In Semaphorin3 A Signaling (2012)
  • Author(s): Hida T, Yamashita N, Usui H, Nakamura F, Sasaki Y, Kikuchi A, Goshima Y
  • Journal Title: J Neurosci Volume: (In press)
  • Peer Reviewed
• [Journal Article] Cartilage acidic protein-1B(LOTUS), an endogenous Nogo receptor antagonist for axon tract formation (2011)
  • Author(s): Sato Y, Iketani M, Kurihara KYamaguchi M, Yamashita N, Nakamura F, Aire Y, Kawasaki T, Hirata T, Abe T, Kiyonari H, Strittmatter SM, Goshima Y, Takei T.
  • Journal Title: Science Volume: 333 Pages: 769-73
  • Peer Reviewed
• [Journal Article] Intranasal administration of Semaphorin-3A alleviates sneezing and nasal rubbing in a murine model of allergic rhinitis (2011)
  • Author(s): Sawaki H, Nakamura F, Aihara M, Nagashima Y, Komori-Yamaguchi J, Yamashita Y, Nakazawa M, Gsohima Y, Ikezawa Z.
  • Journal Title: J Pharmacol Sci Volume: 117 Pages: 34-44
  • NAID: 10029895884
  • Peer Reviewed
• [Journal Article] CRMP5 (collapsin response mediator protein 5) regulates dendritic development and synaptic plasticity in the cerebellar Purkinje cells. (2011)
  • Author(s): Yamashita N, Mosinger B, Roy A, Miyazaki M, Ugajin K, Nakamura F, Sasaki Y, Yamaguchi K, Kolattukudy P, Goshima Y.
  • Journal Title: Journal of Neuroscience Volume: 31 Pages: 1773-1779
  • Peer Reviewed
• [Presentation] 神経ガイドにおけるアクチン結合蛋白質Filamin-AとCRMP相互作用 (2011)
  • Author(s): 中村史雄、肥田友伸、村岡秀紀、小倉顕一、五嶋良郎
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2011-09-16
• [Presentation] 神経ガイドにおけるアクチン結合蛋白質Filamin-AとCRMP相互作用 (2011)
  • Author(s): 中村史雄、肥田友伸、村岡秀紀、小倉顕一、五嶋良郎
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2011-09-16
• [Presentation] PTPδによるc-Srcの脱リン酸化とSema3A情報伝達 (2010)
  • Author(s): 中村史雄、Stephen M Strittmatter、五嶋良郎
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸・神戸コンベンションセンター
  • Year and Date: 2010-09-02
• [Presentation] PTPδによるc-Srcの脱リン酸化とSema3A情報伝達 (2010)
  • Author(s): 中村史雄、Stritmatter SM、五嶋良郎
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸国際会議場(兵庫)
  • Year and Date: 2010-09-02
• [Remarks] 研究結果について特許出願を予定している。