| Project/Area Number |
22650084
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Fusional basic brain science
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ISEKI Mineo 東邦大学, 薬学部, 准教授 (60414009)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,870,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | 光脳科学 / 神経突起 / 神経再生 / 光受容体 / チロシンキナーゼ / Trk / MAPキナーゼ / 神経 / 突起伸長 / フォトトロピン / LOV領域 / Src |
|---|
| Research Abstract |
To explore the optical navigation of neurite outgrowth, I utilized two photoreceptors : bacterial photoactivated adenylyl cyclase(bPAC) and an anonymous photoreceptor X. Transient expression of bPAC in cultured cells showed A-kinase activation upon light stimulation. I designed a fusion protein X-Trk, X-photoreceptor connected with the kinase domain of TrkB. Photostimulation upon the cells expressing X-Trk induced autophosphorylation of X-Trk and MAP kinase activation. Ectopic expression of X-Trk in chick embryonic dorsal root ganglion neurons facilitated the neurite outgrowth upon light stimulation. Enabling the optical navigation of neurite outgrowth with X-Trk may open new avenue to regenerate and analyze neural circuits.
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