Development of an Animal model for stuttering using songbirds

• Project/Area Number: 22650089
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Hokkaido University
• Principal Investigator: WADA Kazuhiro 北海道大学, 大学院・理学研究院, 准教授 (70451408)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,440,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: 疾患モデル / 発話コミュニケーション障害 / コミュニケーション障害 / 吃音 / 感覚運動学習 / 学習臨界期 / ソングバード / 動物モデル / 状況依存的 / 基底核 / 時系列制御 / 発声学習

Research Abstract

In this study, we performed experiments to develop a novel animal model for studying vocal communication disorder: stuttering. For this purpose, we used songbirds that have ability for vocal learning with two approaches, environmental effects and genetic effects.

Research Products

• [Journal Article] Differential androgen receptor expression and DNA methylation state in striatum song nucleus Area X between wild and domesticated songbird strains (2013)
  • Author(s): Wada, K., Hayase, S., Imai, R., Mori, C., Kobayashi, M., Liu, W-C., Takahasi, M., and Okanoya, K
  • Journal Title: European Journal of Neuroscience Volume: (In print)
  • Peer Reviewed
• [Journal Article] Specialized motor-driven dusp1 expression in the song systems of multiple lineages of vocal learning birds (2012)
  • Author(s): Horita H, Kobayashi M, Liu W-C, Oka K, Jarvis ED, Wada K
  • Journal Title: PLoS ONE Volume: 7
  • NAID: 120004689044
  • Peer Reviewed
• [Journal Article] Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue (2012)
  • Author(s): Chen C., Wada K., Jarvis, ED
  • Journal Title: Journal of Visualized Experiments Volume: 62
  • Peer Reviewed
• [Journal Article] Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue. (2012)
  • Author(s): Chen C., Wada K., Jarvis, ED.
  • Journal Title: Journal of Visualized Experiments Volume: 62 Issue: 62
  • DOI: 10.3791/3764
  • Peer Reviewed
• [Journal Article] Specialized motor-driven dusp1 expression in the song systems of multiple lineages of vocal learning birds. (2012)
  • Author(s): Horita H, Kobayashi M, Liu W-C, Oka K, Jarvis ED*, Wada K*.
  • Journal Title: PLoS ONE. Volume: 7 Issue: 8 Pages: e42173-e42173
  • DOI: 10.1371/journal.pone.0042173
  • NAID: 120004689044
  • Peer Reviewed
• [Journal Article] Radioactive in-situ hybridization in avian embryonic tissues (2012)
  • Author(s): Chen, C.-C., Wada, K., Erich D.Jarvis, ED
  • Journal Title: JoVE Volume: (掲載確定)
  • Peer Reviewed
• [Journal Article] 聴覚入力による音声パターン学習・維持の神経機構:学習で獲得された発戸パターンはいかに維持されるか? (2011)
  • Author(s): 堀田悠人, 和多和宏
  • Journal Title: 実験医学 Volume: 29 Pages: 544-550
• [Journal Article] ヒト以外の動物にことばはあるか:ヒトの言語とソングバードの囀り (2011)
  • Author(s): 森千紘, 和多和宏
  • Journal Title: JOHNS Volume: 27 Pages: 1161-1168
• [Journal Article] The dusp1 Immediate Early Gene is regulated by Natural Stimuli Predominantly in Sensory Input Neurons (2010)
  • Author(s): Horita H, Wada K, Rivas MV, Hara E, JarvisED
  • Journal Title: J. Comp. Neurol Volume: 518 Pages: 2873-2901
  • NAID: 120002317143
  • Peer Reviewed
• [Journal Article] Dopamine receptors in a songbird brain (2010)
  • Author(s): Kubikova L, Wada K, Jarvis ED
  • Journal Title: J. Comp. Neurol Volume: 518 Pages: 741-69
  • NAID: 120002317144
  • Peer Reviewed
• [Journal Article] The dusp1 Immediate Early Gene is Regulated by Natural Stimuli Predominantly in Sensory Input Neurons (2010)
  • Author(s): Horita H, Wada K, Rivas MV, Hara E, Jarvis ED
  • Journal Title: Journal of Comparative Neurology Volume: 518 Pages: 2873-2901
  • NAID: 120002317143
  • Peer Reviewed
• [Journal Article] ソングバードの囀りを制御する神経回路・遺伝子 (2010)
  • Author(s): 今井礼夢、森千紘、和多和宏
  • Journal Title: 生物の科学遺伝 Volume: 64 Pages: 40-47
• [Presentation] Songbird as an Animal Models for Vocal Communication Disorder with a View from Nature via Nurture (2013)
  • Author(s): 和多和宏
  • Organizer: 基礎生物学研究所重点共同利用研究シンポジューム
  • Place of Presentation: 岡崎 岡崎カンファレンスセンター
  • Year and Date: 2013-01-24
• [Presentation] Continual vocal behavior influences induction of motor-driven genes in songbird vocal areas (2012)
  • Author(s): 早瀬晋、大串恵理、小林雅比古、和多和宏
  • Organizer: Society for Neuroscience年会
  • Place of Presentation: アメリカ ニューオリンズ Morial Convention Center
  • Year and Date: 2012-10-14
• [Presentation] Variation in gene expression and DNA methylation state in wild and domesticated songbird strains (2012)
  • Author(s): 和多和宏、早瀬晋、今井礼夢、森千紘、小林雅比古、Liu Wan-Chun、高橋美樹、岡ノ谷一夫
  • Organizer: Society for Neuroscience年会
  • Place of Presentation: アメリカ ニューオリンズ Morial Convention Center
  • Year and Date: 2012-10-14
• [Presentation] An inherited genetic program for acquisition of species-specific vocal pattern in songbird (2012)
  • Author(s): 森千紘、和多和宏
  • Organizer: 10th International Congress of Neuroethology
  • Place of Presentation: アメリカ ワシントンDC University of Maryland
  • Year and Date: 2012-08-08
• [Presentation] 鳴禽類ソングバードにおけるウイルス発現系を用いた神経科学研究 (2012)
  • Author(s): 和多和宏
  • Organizer: 包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 仙台 仙台国際センター
  • Year and Date: 2012-07-26
• [Presentation] Epigenetic gene expression dynamics induced by singing regulate a critical period of vocal learning (2011)
  • Author(s): 小林雅比古、今井礼夢、森千紘、大串恵理、堀田悠人、Liu Wan-Chun、和多和宏
  • Organizer: Society for Neuroscience 年会
  • Place of Presentation: アメリカワシントンDCワシントンDCコンベンションセンター
  • Year and Date: 2011-11-14
• [Presentation] Epigenetic gene expression dynamics induced by singing regulate a critical period of vocal learning (2011)
  • Author(s): Nasahiko Kobayashi, Raimu Imai, Chihiro Mori, Eri Ohgushi, Haruhito Horita, Wan-chun Liu, Kazuhiro Wada
  • Organizer: Neuroscience 2011, Society for Neuroscience
  • Place of Presentation: Washigton DC convention center (USA)
  • Year and Date: 2011-11-14
• [Presentation] Restricted learning of heterospecific song in zebra finch (2011)
  • Author(s): R Imai, K Wada
  • Organizer: Neuroscience 2011, Society for Neuroscience
  • Place of Presentation: Washigton DC convention center (USA)
  • Year and Date: 2011-11-13
• [Presentation] Deafening before critical period of vocal learning causes delayed stabilization of vocal patter (2011)
  • Author(s): Chihiro Mori, Wan-chun Liu, Kazuhiro Wada
  • Organizer: Neuroscience 2011, Society for Neuroscience
  • Place of Presentation: Washigton DC convention center (USA)
  • Year and Date: 2011-11-12
• [Presentation] 神経行動科学から考える「生まれと育ち (2011)
  • Author(s): 和多和宏
  • Organizer: ASCON
  • Place of Presentation: 上諏訪諏訪湖ホテル
  • Year and Date: 2011-11-03
• [Presentation] 神経行動科学から考える「生まれと育ち」 (2011)
  • Author(s): 和多和宏
  • Organizer: ASCON
  • Place of Presentation: かたくら諏訪湖ホテル(諏訪市)(招待講演)
  • Year and Date: 2011-11-03
• [Presentation] 囀り学習臨界期中の囀り頻度は囀り日内発達に影響を与える (2011)
  • Author(s): 大串恵理、和多和宏
  • Organizer: 日本動物学会年会
  • Place of Presentation: 旭川大雪クリスタルホール
  • Year and Date: 2011-09-22
• [Presentation] ホルモン投与による囀り行動の制御とその囀り発達への影 (2011)
  • Author(s): 生田麻実、森千紘、和多和宏
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 大雪クリスタルホール(旭川市)
  • Year and Date: 2011-09-22
• [Presentation] 囀り学習臨界期中の囀り頻度は囀り日内発達に影響を与える (2011)
  • Author(s): 大串恵理、和多和宏
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 大雪クリスタルホール(旭川市)
  • Year and Date: 2011-09-22
• [Presentation] 発声学習臨界期における多段階発現制御機構とエピジェネティクス動態 (2011)
  • Author(s): 和多和宏
  • Organizer: 日本動物学会年会
  • Place of Presentation: 旭川大雪クリスタルホール
  • Year and Date: 2011-09-21
• [Presentation] 発声学習臨界期における多段階発現制御機構とエピジェネティクス動態 (2011)
  • Author(s): 和多和宏
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 大雪クリスタルホール(旭川市)(招待講演)
  • Year and Date: 2011-09-21
• [Presentation] 聴覚入力阻害による囀りパターン固定化への影響 (2011)
  • Author(s): 森千紘, 和多和宏
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 大雪クリスタルホール(旭川市)
  • Year and Date: 2011-09-21
• [Presentation] 小鳥のさえずりパターンにおける種特異的拘束 (2011)
  • Author(s): 今井礼夢、和多和宏
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 大雪クリスタルホール(旭川市)
  • Year and Date: 2011-09-21
• [Presentation] Learned heterospecific song patterns were limitedly produced at juvenile stage but finally restricted by species-specific manners in songbird (2011)
  • Author(s): 今井礼夢、和多和宏
  • Organizer: 日本神経科学会年会
  • Place of Presentation: 横浜 パシフィコ横浜
  • Year and Date: 2011-09-16
• [Presentation] Deafening before critical period of vocal learning causes delayed stabilization of vocal patterns (2011)
  • Author(s): 森千紘、和多和宏
  • Organizer: 日本神経科学会年会
  • Place of Presentation: 横浜 パシフィコ横浜
  • Year and Date: 2011-09-16
• [Presentation] 小鳥の囀り学習過程における種特異的な囀りパターンの発達変 (2011)
  • Author(s): 森千紘, 和多和宏
  • Organizer: 日本神経科学会第34回大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-16
• [Presentation] 小鳥の囀り学習過程における種特異的な囀りパターンの発達変化 (2011)
  • Author(s): 今井礼夢、和多和宏
  • Organizer: 日本神経科学会第34回大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-16
• [Presentation] 聴覚入力阻害による囀りパターン固定化への影響 (2011)
  • Author(s): 森千紘、和多和宏
  • Organizer: Animal2011:日本動物心理学会・日本動物行動学会・応用動物行動学会・日本家畜管理 学会合同大
  • Place of Presentation: 東京慶應義塾大学三田キャンパス
  • Year and Date: 2011-09-08
• [Presentation] 聴覚入力阻害による囀りパターン固定化への影響 (2011)
  • Author(s): 森千紘, 和多和宏
  • Organizer: 日本動物心理学会・日本動物行動学会・応用動物行動学会・日本家畜管理学会合同大会
  • Place of Presentation: 慶應義塾大学三田キャンパス(東京都)
  • Year and Date: 2011-09-08
• [Presentation] Epigenetic gene expression dynamics induced by singing regulate the critical period of vocal learning (2011)
  • Author(s): 小林雅比古、今井礼夢、森千紘、Liu Wan-Chun、和多和宏
  • Organizer: 日本エピジェネティクス研究会年会
  • Place of Presentation: 熊本KKRホテル熊本
  • Year and Date: 2011-05-19
• [Presentation] 発声行動依存的に誘導されるエピジェネティックな脳内遺伝子発現調節が音声発声学習の臨界期制御に関与する (2011)
  • Author(s): 小林雅比古・今井礼夢・森千紘・Wanchun Liu・和多和宏
  • Organizer: 日本エピジェネティクス研究会
  • Place of Presentation: KKRホテル熊本(熊本県)
  • Year and Date: 2011-05-19
• [Presentation] 神経行動科学から考える「生まれと育ち」 (2011)
  • Author(s): 和多和宏
  • Organizer: 脳と心のメカニズム(第11回冬のワークショップ)
  • Place of Presentation: ルスツ 招待講演
  • Year and Date: 2011-01-12
• [Presentation] Deafening before critical period for vocal learning affects gene expression pattern in song nuclei of songbird (2010)
  • Author(s): 森千紘、和多和宏
  • Organizer: Society for Neuroscience 年会
  • Place of Presentation: アメリカサンディエゴサンディエゴコンベンションセンター
  • Year and Date: 2010-11-16
• [Presentation] Expression dynamics of epigenetics-related genes developmentally regulated by singing behavior (2010)
  • Author(s): Masahiko Kobayashi, Raimu Imai, Chihiro Mori, Haruhito Horita, Wan-chun Liu, Kazuhiro Wada
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-16
• [Presentation] Deafening before critical period for vocal learning affects gene expression pattern in song nuclei of songbird. (2010)
  • Author(s): Chihiro Mori, Kazuhiro Wada
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-16
• [Presentation] Differential expression pattern of singing induced Egr family through sensorimotor period of song learning. (2010)
  • Author(s): Raimu Imai, Ohgushi Eri, Kazuhiro Wada
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-16
• [Presentation] Repeated evolution of activity-dependent motor-driven dusp1 expression in song nuclei of vocal learning birds (2010)
  • Author(s): Haruhito Horita, Masahiko Kobayashi, Kotaro Oka, Erich D.Jarvis, Kazuhiro Wada
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-15
• [Presentation] Specialized motor-driven dusp1 expression in song nuclei of vocal learning birds (2010)
  • Author(s): 堀田悠人、小林雅比古、Jarvis Erich、 岡浩太郎、和多和宏
  • Organizer: 日本神経科学会年会
  • Place of Presentation: 神戸 神戸国際会議場
  • Year and Date: 2010-09-03
• [Presentation] Specialized motor-driven dusp1 expression in song nuclei of vocal learning birds. (2010)
  • Author(s): Haruhito Horita, Masahiko Kobayashi, Erich D.Jarvis, Kotaro Oka, Kazuhiro Wada
  • Organizer: 日本神経科学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] キンカチョウの囀り行動頻度による囀り発達の影響 (2010)
  • Author(s): 大串恵理、和多和宏
  • Organizer: 日本動物学会北海道支部
  • Place of Presentation: 旭川
  • Year and Date: 2010-08-21
• [Presentation] 囀り行動によって発現誘導されるエピジェネティクス関連遺伝子群の学習臨界期中の発現変化 (2010)
  • Author(s): 小林雅比古、和多和宏
  • Organizer: 日本動物学会北海道支部
  • Place of Presentation: 旭川
  • Year and Date: 2010-08-21
• [Presentation] 音声発声学習における聴覚入力阻害による行動発達への影響 (2010)
  • Author(s): 森千紘、和多和宏
  • Organizer: 日本動物学会北海道支部
  • Place of Presentation: 旭川
  • Year and Date: 2010-08-21
• [Presentation] 新規音声時系列構造の開発 (2010)
  • Author(s): 今井礼夢、和多和宏
  • Organizer: 日本動物学会北海道支部
  • Place of Presentation: 旭川
  • Year and Date: 2010-08-21
• [Presentation] 発声行動依存的に誘導されるエピジェネティクス関連遺伝子ヒストンH3.3の学習臨界期間における脳内発現変化 (2010)
  • Author(s): 小林雅比古、和多和宏
  • Organizer: 日本エピジェネティクス研究会
  • Place of Presentation: 鳥取
  • Year and Date: 2010-05-29
• [Presentation] Temporal-spatial regulation of singing-induced genes and epigenetic dynamics in the critical period for vocal learning in songbirds
  • Author(s): Kazuhiro Wada
  • Organizer: 新学術領域研究3領域合同国際シンポジューム
  • Place of Presentation: 東京, 東京大
  • Invited
• [Presentation] Continual vocal behavior influences induction of motor-driven genes in songbird vocal areas
  • Author(s): Shin Hayase, Eri Ohgushi, Masahiko Kobayashi, Kazuhiro Wada
  • Organizer: SFN Neuroscience 2012
  • Place of Presentation: New Orleans N. Morial Convention Center, USA
• [Presentation] Variation in gene expression and DNA methylation state in wild and domesticated songbird strains
  • Author(s): Kazuhiro Wada, Shin Hayase, Raimu Imai, Chihiro Mori, Masahiko Kobayashi, Wan-chun Liu, Miki Takahasi, and Kazuo Okanoya
  • Organizer: SFN Neuroscience 2012
  • Place of Presentation: New Orleans N. Morial Convention Center, USA
• [Presentation] 鳴禽類の発声学習における囀りパターン固定化の 神経機構
  • Author(s): 森千紘, 和多和宏
  • Organizer: 日本動物学会北海道支部第57回大会
  • Place of Presentation: 札幌, 北海道大学
• [Presentation] 囀りパターン学習時の種特異的バイアスを与える大脳皮質・基底核・視床ループの役割
  • Author(s): 今井礼夢, 和多和宏
  • Organizer: 日本動物学会北海道支部第57回大会
  • Place of Presentation: 札幌, 北海道大学
• [Presentation] 継続的な発声行動は遺伝子発現誘導率を脳部位選択的に変化させる
  • Author(s): 早瀬晋, 大串恵理, 和多和宏
  • Organizer: 日本動物学会北海道支部第57回大会
  • Place of Presentation: 札幌, 北海道大学
• [Presentation] A role of the basal ganglia-forebrain circuit for biasing species-specific begavioral patterns
  • Author(s): Raimu Imai, Kazuhiro Wada
  • Organizer: 10th International Congress of Neuroethology
  • Place of Presentation: University of Maryland, USA
• [Presentation] An inherited genetic program for acquisition of species-specific vocal pattern in songbird
  • Author(s): Mori C, Liu W and Wada K
  • Organizer: 10th International Congress of Neuroethology
  • Place of Presentation: University of Maryland, USA
• [Presentation] Epigenetic gene expression dynamics induced by singing in a critical period of vocal learning
  • Author(s): Kazuhiro Wada , Masahiko Kobayashi, Wan-chun Liu
  • Organizer: 10th International Congress of Neuroethology
  • Place of Presentation: University of Maryland, USA
• [Presentation] 継続的な発声行動は遺伝子発現誘導率を脳部位選択的に変化させる
  • Author(s): 早瀬晋、大串恵理、小林雅比古、和多和宏
  • Organizer: 包括脳ネットワーク 夏のワークショップ
  • Place of Presentation: 仙台・仙台国際センター
• [Presentation] 鳴禽類ソングバードにおけるウイルス発現系を用いた神経科学研究
  • Author(s): 和多和宏
  • Organizer: 包括脳ネットワーク 夏のワークショップ
  • Place of Presentation: 仙台・仙台国際センター
  • Invited
• [Presentation] Songbird as an Animal Models for Vocal Communication Disorder with a View from Nature via Nurture
  • Author(s): Kazuhiro Wada
  • Organizer: 基礎生物科学研究所重点共同利用研究シンポジューム
  • Place of Presentation: 岡崎, 岡崎カンファレンスセンター
  • Invited
• [Book] 遊ぶ (2012)
  • Author(s): 中村桂子
  • Publisher: 新曜社
• [Book] 生命誌年刊号vol.69-72「遊ぶ」 (2012)
  • Author(s): 中村 桂子
  • Total Pages: 275
  • Publisher: 新曜社
• [Book] 行動遺伝学入門 (2011)
  • Author(s): 小出剛・山元大輔 編著
  • Total Pages: 217
  • Publisher: 裳華房
• [Remarks]
  • URL: http://www.wada-lab.org/