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Establishment of fluorescent in vivo Western method

Research Project

Project/Area Number 22650092
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionUniversity of Tsukuba

Principal Investigator

MIWA Yoshihiro  筑波大学, 医学医療系, 講師 (70263845)

Co-Investigator(Kenkyū-buntansha) TANAKA Junko  筑波大学, 医学医療系, 助教 (30517793)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,320,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsイメージング / 近赤外 / 蛍光 / 動物実験技術 / 分子認識 / バイオテクノロジー / 遺伝子組換え / 細胞・組織 / 神経科学
Research Abstract

Fluorescent real-time imaging methods enables us to monitor proteins and other molecules in living animals. This technique can make significant contribution to not only basic science but also researches for drug discovery, regenerative medicine and clinical state analyses in model animals. For this purpose, we succeeded to develop a non-fluorescent in near-infrared and nutritionally complete diet and a fluorescent protein derivative which shows 30-fold higher intensity of NIR fluorescence.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (31 results)

All 2012 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (22 results) Book (1 results)

  • [Journal Article] Archipelago architecture of focal adhesion : Membrane molecules freely enter and exit from the focal adhesion zone2012

    • Author(s)
      Shibata ACE, Fujiwara TK, Chen L, Suzuki KGN, Ishikawa Y, Miwa Y, Chadda R, Naruse K, Kusumi A
    • Journal Title

      Cytoskeleton

      Volume: in press

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Environmental polarity estimation in living cells by use of quinoxaline-based full-colored solvatochromic fluorophore PQX and its derivatives2012

    • Author(s)
      Kudo K, Momotake A, Tanaka JK, Miwa Y, Arai T
    • Journal Title

      Photochem Photobiol Sci

      Volume: 11 Pages: 674-678

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Archipelago architecture of focal adhesion : Membrane molecules freely enter and exit from the focal adhesion zone2012

    • Author(s)
      Shibata ACE, et al.
    • Journal Title

      Cytoskeleton

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Environmental polarity estimation in living cells by use of quinoxaline-based full-colored solvatochromic fluorophore PQX and its derivatives2012

    • Author(s)
      Kudo, et al
    • Journal Title

      Photochem Photobiol Sci

      Volume: 11 Issue: 4 Pages: 674-678

    • DOI

      10.1039/c2pp05337c

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Elliptic curves and Fibonacci numbers arising from Lindenmayer system with symbolic computation2011

    • Author(s)
      Yoshida H, Miwa Y, Kaneko M
    • Journal Title

      APPLICABLE ALGEBRA in ENGINEERING, COMMUNICATION and COMPUTING.

      Volume: 22 Pages: 147-164

    • NAID

      120003783219

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Algebraic approaches to underdeterminated experiments in biology2010

    • Author(s)
      Yoshida H, Kimura K, Yoshida N, Tanaka J, Miwa Y
    • Journal Title

      IPSJ Transactions on Bioinformatics

      Volume: 3 Pages: 62-69

    • NAID

      130000418482

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tsukuba-green : A fluorescent dye that emits Green fluorescence useful forlive-cell imaging2010

    • Author(s)
      Senda, N., Miwa, Y., Tanaka, J., Momotake, A. and Arai, T
    • Journal Title

      Chem. Lett

      Volume: 39 Pages: 308-310

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Algebraic approaches to underdeterminaed experiments in biology.2010

    • Author(s)
      Yoshida, et al.
    • Journal Title

      IPSJ Transactions on Bioinformatics

      Volume: 3 Pages: 62-69

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Development of novel fluorescence imaging systems through integrated research field of various disciplines2012

    • Author(s)
      三輪佳宏
    • Organizer
      NAIST Colloquium for Future-Pioneering、招待講演
    • Place of Presentation
      奈良
    • Year and Date
      2012-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Eepisomal型ベクターを用いたマルチカラーイメージングの新展開2012

    • Author(s)
      三輪佳宏
    • Organizer
      農芸化学会2012ランチョンセミナー和光純薬
    • Place of Presentation
      京都大学
    • Year and Date
      2012-03-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 近赤外蛍光を用いたマウス非侵襲whole bodyイメージング2012

    • Author(s)
      三輪佳宏
    • Organizer
      日本顕微鏡学会バイオメディカルニューマイクロスコープ分科会
    • Place of Presentation
      帝京大学
    • Year and Date
      2012-03-06
    • Related Report
      2011 Final Research Report
  • [Presentation] 蛍光バイオイメージングの実際2012

    • Author(s)
      三輪佳宏
    • Organizer
      つくば学際ワークショップ
    • Place of Presentation
      筑波大学
    • Year and Date
      2012-01-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Establishment of NIR fluorescent in toto imaging system2011

    • Author(s)
      三輪佳宏, 田中順子
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      ポスター(横浜)
    • Year and Date
      2011-12-11
    • Related Report
      2011 Final Research Report
  • [Presentation] ケミカルスクリーニングに向けたハイスループットEGFシグナル検出プローブの開発2011

    • Author(s)
      田中順子, 杉山結香, 石上進太郎, 吉野哲哉, 伊藤彰英, 中尾洋一, 三輪佳宏
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-11
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Establishment of NIR fluorescent in toto imaging system2011

    • Author(s)
      三輪佳宏
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 共創研究!化学とバイオイメージング2011

    • Author(s)
      三輪佳宏
    • Organizer
      日本化学会生体機能関連化学部会若手の会、招待講演
    • Place of Presentation
      つくば
    • Year and Date
      2011-09-11
    • Related Report
      2011 Final Research Report
  • [Presentation] 共創研究!化学とバイオイメージング2011

    • Author(s)
      三輪佳宏
    • Organizer
      日本化学会生体機能関連化学部会若手の会
    • Place of Presentation
      つくば(招待講演)
    • Year and Date
      2011-09-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 蛍光in totoイメージングの現状と応用と展望2011

    • Author(s)
      三輪佳宏, 田中順子
    • Organizer
      酸化ストレス研究会、招待講演
    • Place of Presentation
      館山
    • Year and Date
      2011-08-09
    • Related Report
      2011 Final Research Report
  • [Presentation] 蛍光in totoイメージングの現状と応用と展望2011

    • Author(s)
      三輪佳宏
    • Organizer
      日本酸化ストレス研究会
    • Place of Presentation
      館山(招待講演)
    • Year and Date
      2011-08-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] 創薬支援の蛍光イメージング2011

    • Author(s)
      三輪佳宏、田中順子
    • Organizer
      大阪大学特別講演会、招待講演
    • Place of Presentation
      大阪大学
    • Year and Date
      2011-07-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 創薬支援の蛍光イメージング2011

    • Author(s)
      三輪佳宏
    • Organizer
      大阪大学特別講演会
    • Place of Presentation
      大阪(招待講演)
    • Year and Date
      2011-07-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] マウス3次元蛍光イメージング2010

    • Author(s)
      三輪佳宏, 田中順子
    • Organizer
      BMB2010、ポスター
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2011 Final Research Report
  • [Presentation] 蛍光寿命測定FCMを用いたFRETシステムの利用2010

    • Author(s)
      田中順子, 三輪佳宏
    • Organizer
      BMB2010、ポスター
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2011 Final Research Report
  • [Presentation] Episomal型ベクターを用いたマルチカラーイメージングの新展開2010

    • Author(s)
      三輪佳宏
    • Organizer
      BMB2010、招待講演
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
    • Related Report
      2011 Final Research Report
  • [Presentation] 初めての蛍光3Dイメージングを成功させるTips2010

    • Author(s)
      三輪佳宏
    • Organizer
      第5回IVIS Imaging Systemユーザー会、招待講演
    • Place of Presentation
      東京
    • Year and Date
      2010-10-06
    • Related Report
      2011 Final Research Report
  • [Presentation] 初めての蛍光3Dイメージングを成功させるTips2010

    • Author(s)
      三輪佳宏
    • Organizer
      第5回IVIS Imaging Systemユーザー会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生体イメージング:細胞から動物まで2010

    • Author(s)
      三輪佳宏
    • Organizer
      RCNP研究会、招待講演
    • Place of Presentation
      大阪
    • Year and Date
      2010-10-01
    • Related Report
      2011 Final Research Report
  • [Presentation] 異分野交流を通じた生体イメージング2010

    • Author(s)
      三輪佳宏
    • Organizer
      ぶんせき秘帖(日本分析化学会若手の会)、招待講演
    • Place of Presentation
      大阪府羽衣青少年センター
    • Year and Date
      2010-08-11
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス蛍光ライブイメージングの基礎2010

    • Author(s)
      三輪佳宏
    • Organizer
      第11回Wakoつくばフォーラム、招待講演
    • Place of Presentation
      つくば
    • Year and Date
      2010-07-13
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス生体イメージングによる薬物動態解析2010

    • Author(s)
      三輪佳宏
    • Organizer
      招待講演
    • Place of Presentation
      金沢
    • Year and Date
      2010-05-21
    • Related Report
      2011 Final Research Report
  • [Book] 蛍光タンパク質を利用したデグラトンプローブ2010

    • Author(s)
      田中順子、三輪佳宏
    • Publisher
      NTS酵素利用技術体系
    • Related Report
      2011 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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