Development of biosensing technology using cell-derived liposomes

• Project/Area Number: 22650105
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: YASUDA Takashi 九州工業大学, 大学院・生命体工学研究科, 准教授 (80270883)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,280,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: リポソーム / 膜タンパク質 / GPCR / QCM / マイクロ流路 / リンパ球細胞 / バイオセンサ / 細胞刺激 / マイクロ流体デバイス / 微小孔 / ヒトリンパ球細胞 / 細胞周期制御

Research Abstract

Liposomes were produced and separated from human lymphocytes using chemical stimulation in a microhole array devise, and the number of produced liposomesand the separation efficiency were dramatically increased. Also, liposomes havingspecific membrane proteins that were expressed in the host lymphocytes by gene transfection were immobilized on an electrode of a QCM (quartz crystal microbalance) sensor, and interactions between the membrane proteins and their ligands were detected

Research Products

• [Journal Article] QCM Sensor Using Membrane Proteins of Cell-derived Liposomes (2013)
  • Author(s): 山中誠, 安田隆
  • Journal Title: IEEJ Transactions on Sensors and Micromachines Volume: 133 Issue: 5 Pages: 155-156
  • DOI: 10.1541/ieejsmas.133.155
  • NAID: 10031167011
  • ISSN: 1341-8939, 1347-5525
  • Peer Reviewed
• [Presentation] QCM Detection of Membrane Protein-ligand Interactions Using Cell-derived Liposomes (2012)
  • Author(s): Makoto Yamanaka, Takashi Yasuda
  • Organizer: The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2012)
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県)
  • Year and Date: 2012-10-29
• [Presentation] 細胞由来リポソームを用いた膜タンパク質-リガンド相互作用のQCM計測 (2012)
  • Author(s): 山中誠, 安田隆
  • Organizer: 第29回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 北九州国際会議場(福岡県)
  • Year and Date: 2012-10-24
• [Presentation] 薬剤刺激によるヒト細胞由来リポソームの直接生成 (2012)
  • Author(s): 山中誠
  • Organizer: 日本機械学会第24回バイオエンジニアリング講演会
  • Place of Presentation: 大阪大学(大阪府)
  • Year and Date: 2012-01-07
• [Presentation] QCM Detection of Membrane Protein-ligand Interactions Using Cell-derived Liposomes (2012)
  • Author(s): Makoto Yamanaka
  • Organizer: The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2012)
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県）
• [Presentation] QCM Detection of Membrane Protein-ligand Interactions Using Cell-derived Liposomes (2012)
  • Author(s): Makoto Yamanaka
  • Organizer: The Fifth Japan-Korea Joint Symposium on Bio-microsensing Technology (5th JKBT)
  • Place of Presentation: 九州工業大学（福岡県）
• [Presentation] 細胞由来リポソームを用いた膜タンパク質－リガンド相互作用のQCM計測 (2012)
  • Author(s): 山中誠
  • Organizer: 第29回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 北九州国際会議場（福岡県）
• [Presentation] 細胞由来リポソームを用いた膜タンパク質－リガンド相互作用のQCM計測 (2012)
  • Author(s): 山中誠
  • Organizer: 第51回日本生体医工学会大会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] ヒト細胞由来リポソームを用いた膜タンパク質修飾QCMセンサ (2011)
  • Author(s): 山中誠
  • Organizer: 第24回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 大阪府立大学(大阪府)
  • Year and Date: 2011-11-18
• [Presentation] QCM Detection of Protein-ligand Interactions Using Cell-derived Liposomes (2011)
  • Author(s): Makoto Yamanaka
  • Organizer: The Fourth Japan-Korea Joint Symposium on Biomicrosensing Technology (4th JKBT)
  • Place of Presentation: 九州工業大学(福岡県)
  • Year and Date: 2011-10-28
• [Presentation] 微小孔デバイスを用いたヒト細胞由来リポソームの生成分離 (2011)
  • Author(s): 山中誠, 安田隆
  • Organizer: ナノ学会第9回大会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2011-06-03
• [Presentation] Microhole Device for Derivation and Separation of Liposomes from Human Lymphocytes with Synchronized Culture (2010)
  • Author(s): Makoto Yamanaka, Takashi Yasuda
  • Organizer: The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2010)
  • Place of Presentation: Groningen(オランダ)
  • Year and Date: 2010-10-06
• [Presentation] Microhole Device for Derivation and Separation of Liposomes from Human Lymphocytes with Synchronized Culture (2010)
  • Author(s): Makoto Yamanaka
  • Organizer: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2010)
  • Place of Presentation: オランダ・フローニンゲン
  • Year and Date: 2010-10-06
• [Presentation] 微小孔アレイデバイスを用いたヒトリンパ球細胞からのリポソーム生成分離 (2010)
  • Author(s): 山中誠
  • Organizer: FAIS-SAGA-LS合同シンポジウム
  • Place of Presentation: 北九州
  • Year and Date: 2010-07-20
• [Presentation] Microhole Array Device for Derivation and Separation of Liposomes from Human Lymphocytes (2010)
  • Author(s): Makoto Yamanaka
  • Organizer: The 49th Annual Conference of Japanese Society for Medical and Biological Engineering
  • Place of Presentation: 大阪
  • Year and Date: 2010-06-25
• [Presentation] 微小孔アレイデバイスを用いたヒトリンパ球細胞からのリポソーム生成分離 (2010)
  • Author(s): 山中誠
  • Organizer: 電気学会センサ・マイクロマシン部門 平成22年度総合研究会 バイオ・マイクロシステム研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-18
• [Remarks] 研究紹介「リポソーム・デバイス」
• [Remarks] 研究紹介「リポソーム・デバイス」
  • URL: http://www.life.kyutech.ac.jp/~yasuda/jp/research/liposome/index.html
• [Remarks]
  • URL: http://www.life.kyutech.ac.jp/~yasuda/jp/research/index.html