Development of biosensing technology using cell-derived liposomes
Project/Area Number |
22650105
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Biomedical engineering/Biological material science
|
Research Institution | Kyushu Institute of Technology |
Principal Investigator |
YASUDA Takashi 九州工業大学, 大学院・生命体工学研究科, 准教授 (80270883)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,280,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
Keywords | リポソーム / 膜タンパク質 / GPCR / QCM / マイクロ流路 / リンパ球細胞 / バイオセンサ / 細胞刺激 / マイクロ流体デバイス / 微小孔 / ヒトリンパ球細胞 / 細胞周期制御 |
Research Abstract |
Liposomes were produced and separated from human lymphocytes using chemical stimulation in a microhole array devise, and the number of produced liposomesand the separation efficiency were dramatically increased. Also, liposomes havingspecific membrane proteins that were expressed in the host lymphocytes by gene transfection were immobilized on an electrode of a QCM (quartz crystal microbalance) sensor, and interactions between the membrane proteins and their ligands were detected
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Report
(4 results)
Research Products
(19 results)