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Preparation of cell-based electron-generator

Research Project

Project/Area Number 22650107
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionThe University of Tokyo

Principal Investigator

ISHIHARA Kazuhiko  東京大学, 大学院・工学系研究科, 教授 (90193341)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,350,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsバイオ起電デバイス / リン脂質ポリマー / 自発形成ゲル / 電子移動メディエーター / バクテリア / 細胞ベース起電デバイス / 電子メディエーター / ポリマーハイドロゲル / シュワネラ菌 / 細胞親和性 / 可逆的ゲル化反応 / バイオマテリアル / 細胞親和型ポリマー / 酸化還元反応 / 細胞固定化 / 電子移動反応
Research Abstract

The biological and electrochemical properties of redox phospholipid polymer hydrogel containing electron-generating bacterium(Shewanella oneidensis MR-1) were investigated herein to understand a fundamental knowledge of materials for developing a microbial fuel cell. A water-soluble and amphiphilic phospholipid polymer, poly[ 2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid-co-vinylferrocene](PMBVF), used as one of the components of a hydrogel matrix for encapsulation of bacteria and an electron transfer mediator, was synthesized. The hydrogel was formed spontaneously and encapsulated shewanella three-dimensionally when an aqueous solution of PMBVF suspended with the bacterium and an aqueous solution of poly(vinyl alcohol)(PVA) were gently mixed together. Viability evaluation demonstrated that the shewanella encapsulated in the PMBVF/PVA hydrogel maintained high viability levels even after long-term storage and the shewanella made good metabolism. The amperometric measurement showed that the PMBVF/PVA hydrogel could maintain the electron transfer efficiency even when shewanella was encapsulated. Thus, the PMBVF/PVA hydrogel not only provided a mild environment for long-term bacterial survival but also maintained the electron transfer efficiency from the shewanella to the electrode. We concluded that the hydrogel/bacteria hybrid biomaterials, such as PMBVN/PVA/shewanella, have promising application to fabricate living cell-based or bacterium-based biodevices.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (18 results)

All 2012 2011 2010 Other

All Journal Article (5 results) (of which Peer Reviewed: 2 results) Presentation (10 results) Remarks (3 results)

  • [Journal Article] Fabrication of polymeric electron-transfer mediator/enzyme hydrogel multilayer on an Au electrode in a layer-by-layer process2012

    • Author(s)
      Z. Li, T. Konno, M. Takai, K. Ishihara
    • Journal Title

      Biosensor Bioelectron

      Volume: 34(1) Pages: 191-196

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Enzyme oxidase-immobilized phospholipid polymer microparticles for biofuel cell application2011

    • Author(s)
      X. Lin, T. Konno, M. Takai, K. Ishihara
    • Journal Title

      Trans Mater Res Soc

      Volume: 36(3) Pages: 531-534

    • NAID

      130003399013

    • Related Report
      2011 Final Research Report
  • [Journal Article] Layer-by-layer building up of redox phospholipid polymer hydrogel electrode for biosensor2011

    • Author(s)
      Z. Li, T. Konno, M. Takai, K. Ishihara
    • Journal Title

      Trans Mater Res Soc

      Volume: 36(3) Pages: 545-548

    • NAID

      130003399016

    • Related Report
      2011 Final Research Report
  • [Journal Article] Enzyme oxidase-immobilized phospholipid polymer microparticles for biofuel cell application2011

    • Author(s)
      X.Lin, T.Konno, M.Takai, K.Ishihara
    • Journal Title

      Trans Mater Res Soc Jan

      Volume: 36(3) Pages: 531-534

    • NAID

      130003399013

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Layer-by-layer building up of redox phospholipid polymer hydrogel electrode for biosensor2011

    • Author(s)
      Z.Li, T.Konno, M.Takai, K.Ishihara
    • Journal Title

      Trans Mater Res Soc Jan

      Volume: 36(3) Pages: 545-548

    • DOI

      10.1007/12_2011_151

    • NAID

      130003399016

    • ISBN
      9783642278556, 9783642278563
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Encapsulation of Shewanella in the Redox Phospholipid Polymer Hydrogelfor Microbial Fuel Cell Fabrication2011

    • Author(s)
      X. Lin, T. Konno, M. Takai, K. Ishihara
    • Organizer
      MRS-J年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-19
    • Related Report
      2011 Final Research Report
  • [Presentation] Encapsulation of Shewanella in the Redox Phospholipid Polymer Hydrogel for Microbial Fuel Cell Fabricationv2011

    • Author(s)
      X.Lin, T.Konno, M.Takai, K.Ishihara
    • Organizer
      MRS-J年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] Enzyme Oxidase-immobilized Phospholipid Polymer Microparticles for Biofuel Cell Fabrication2011

    • Author(s)
      X. Lin, T. Konno, M. Takai, K. Ishihara
    • Organizer
      3rd Asian Biomaterials Congress
    • Place of Presentation
      プサン(韓国)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Layer-by-Layer Building Up of Redox Phospholipid Polymer Hydrogel Electrode2011

    • Author(s)
      Z. Li, T. Konno, M. Takai, K. Ishihara
    • Organizer
      3rd Asian Biomaterials Congress
    • Place of Presentation
      プサン(韓国)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Enzyme oxidase-immobilized phospholipid polymer microparticles for bio図2.ゲルの網目構造(A)と固定化されたシュワネラ菌(B)図3. PMBVF/PVA/シュワネラ菌電極の起電特性fuel cell application2011

    • Author(s)
      X. Lin, T. Konno, M. Takai, K. Ishihara
    • Organizer
      高分子学会年次大会
    • Place of Presentation
      大阪
    • Year and Date
      2011-05-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Enzyme oxidase-immobilized phospholipid polymer microparticles for biofuel cell application2011

    • Author(s)
      X.Lin, T.Konno, M.Takai, K.Ishihara
    • Organizer
      高分子学会年次大会
    • Place of Presentation
      大阪
    • Year and Date
      2011-05-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Layer-by-Layer Building up of Redox Phospholipid Polymer Hydrogel Electrode for Biosensor2010

    • Author(s)
      Z. Li, T. Konno, M. Takai, K. Ishihara
    • Organizer
      日本MRS学会学術大会
    • Place of Presentation
      横浜
    • Year and Date
      2010-12-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Oxidase-immobilized polymer microparticles coated with MPC polymer for biofuel cell application2010

    • Author(s)
      X. Lin, T. Konno, M. Takai, K. Ishihara
    • Organizer
      日本MRS学会学術大会
    • Place of Presentation
      横浜
    • Year and Date
      2010-12-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Layer-by-Layer Building up of Redox Phospholipid Polymer Hydrogel Electrode for Biosensor2010

    • Author(s)
      Ziyin Li, Tomohiro Konno, Madoka Takai, Kazuhiko Ishihara
    • Organizer
      日本MRS学会学術大会
    • Place of Presentation
      横浜開港記念会館
    • Year and Date
      2010-12-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Oxidase-immobilized polymer microparticles coated with MPC polymerfor biofuel cell application2010

    • Author(s)
      Xiaojie Lin, Tomohiro Konno, Madoka Takai, Kazuhiko Ishihara
    • Organizer
      日本MRS学会学術大会
    • Place of Presentation
      横浜開港記念会館
    • Year and Date
      2010-12-21
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.mpc.t.u-tokyo.ac.jp/ishihara/theme/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.mpc.t.u-tokyo.ac.jp/ishihara/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.mpc.t.u-tokyo.ac.jp

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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