Development of a novel protein delivery system using peroxisomes

• Project/Area Number: 22650111
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Osaka University
• Principal Investigator: TANIZAWA Katsuyuki 大阪大学, 産業科学研究所, 教授 (20133134)
• Co-Investigator(Kenkyū-buntansha): MATSUZAKI Takashi 大阪大学, 医学研究科, 特任研究員 (90456939)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,240,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: タンパク質送達システム / 細胞生物学 / ペルオキシソーム

Research Abstract

In this study, we developed a novel protein delivery system using peroxisome and the peroxisomal protein import system as a protein delivery carrier and a protein concentrator, respectively. First, we established an affinity purification method for highly purified peroxisomes in which the target protein had been efficiently imported intracellularly. Next, we developed an efficient method to display specific antibodies onto the surface of the purified peroxisomes and utilized it to an antibody-dependent and functional delivery of the target protein encapsulated in the peroxisomes. Finally, we successfully demonstrated that α-galactosidase A, a therapeutic protein agent for Fabry disease, could be efficiently delivered into the target lysosomes by using this novel protein delivery system.

Research Products

• [Journal Article] Hepatitis B Virus Envelope L Protein-Derived Bio-Nanocapsules : Mechanisms of Cellular Attachment and Entry into Human Hepatic Cells (2012)
  • Author(s): M. Yamada, A. Oeda, J. Jung, M. Iijima, N. Yoshimoto, T. Niimi, S.-Y. Jeong, E.K. Choi, K. Tanizawa, and S. Kuroda
  • Journal Title: Journal of Controlled Release Volume: 160 Issue: 2 Pages: 322-329
  • DOI: 10.1016/j.jconrel.2011.11.004
  • Peer Reviewed
• [Journal Article] Fluorophore-labeled nanocapsules displaying IgG Fc-binding domains for the simultaneous detection of multiple antigens (2011)
  • Author(s): M.Iijima, T.Matsuzaki, N.Yoshimoto, T.Niimi, K.Tanizawa, S.Kuroda
  • Journal Title: Biomaterials Volume: 32 Issue: 34 Pages: 9011-9020
  • DOI: 10.1016/j.biomaterials.2011.08.012
  • Peer Reviewed
• [Journal Article] Nanocapsules Incorporating IgG Fc-binding Domain Derived from Staphylococcus aureus Protein-A for Displaying IgGs on Immunosensor Chips (2011)
  • Author(s): M.Iijima, H.Kadoya, T.Matsuzaki, S.Hatahira, S.Hiramatsu, G.Jung, A.Martin, J.Quinn, J.Jung, S.-Y.Jeong, E.K.Choi, T.Arakawa, F.Hinako, M.Kusunoki, N.Yoshimoto, T.Niimi, K.Tanizawa, S.Kuroda
  • Journal Title: Biomaterials Volume: 32 Issue: 6 Pages: 1455-1464
  • DOI: 10.1016/j.biomaterials.2010.10.057
  • Peer Reviewed