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Gene transfer system into mesenchymal stem cell by peptide-modified liposomes and ultrasound technology

Research Project

Project/Area Number 22650119
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Medical systems
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

NEGISHI Yoichi  東京薬科大学, 薬学部, 准教授 (50286978)

Co-Investigator(Kenkyū-buntansha) ARAMAKI Yukihiko  東京薬科大学, 薬学部, 教授 (90138959)
TAKAHASHI Yoko  東京薬科大学, 薬学部, 助手 (30453806)
Co-Investigator(Renkei-kenkyūsha) TAKAGI Norio  東京薬科大学, 薬学部, 准教授 (50318193)
MARUYAMA Kazuo  帝京大学, 薬学部, 教授 (30130040)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,140,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥800,000 (Direct Cost: ¥800,000)
Keywordsペプチド修飾リポソーム / 超音波照射 / 遺伝子細胞療法 / バブルリポソーム / 超音波 / リポソーム
Research Abstract

In this study, to enhance transfection efficiency by promoting the escape of the liposomes from the endosome to the cytosol and the nuclear localization of plasmid DNA, nuclear localization signals (NLS) peptides were used for condensing plasmid DNA encoding luciferase (pCMV-Luc) and embeding it into AG73-PEG liposomes. As a result, AG73-PEG liposomes showed selective gene delivery to cellsvia syndecan-2. Efficient gene transfection was observed. The efficacy was further enhanced in the combination of Bubble liposomes and US exposure. Thus, the combination of AG73-PEG liposomes with NLS peptides and BLs and US may be useful method to achieve selective and efficient gene delivery. This method may be also expected to a gene transfection system to mesenchymal stem cell in application of ex vivo non-viral gene therapy

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (4 results)

All 2012 2011

All Presentation (4 results)

  • [Presentation] 核移行シグナルペプチド内封AG73修飾リポソームによる新規遺伝子デリバリーツールの開発2012

    • Author(s)
      根井彰浩,根岸洋一,高橋佐慧子,小俣大樹,鈴木 亮,丸山一雄,野水基義,新槇幸彦
    • Organizer
      アンチセンス・遺伝子・デリバリーシンポジウム2012
    • Year and Date
      2012-09-25
    • Related Report
      2012 Final Research Report
  • [Presentation] 核移行シグナルペプチド内封AG73修飾リポソームによる新規遺伝子デリバリーツールの開発2012

    • Author(s)
      根井 彰浩、根岸 洋一、高橋 佐慧子、小俣 大樹、鈴木 亮、丸山 一雄、野水 基義、新槇 幸彦
    • Organizer
      アンチセンス・遺伝子・デリバリーシンポジウム2012
    • Place of Presentation
      仙台
    • Related Report
      2012 Annual Research Report
  • [Presentation] バブルリポソーム併用超音波遺伝子導入法を用いた間葉系幹細胞への遺伝子導入効果の解析2011

    • Author(s)
      鈴木健太郎, 根岸洋一, 高橋葉子, 高橋良太, 鈴木 亮, 丸山一雄, 新槇幸彦
    • Organizer
      日本薬学会第131 年会
    • Place of Presentation
      静岡
    • Year and Date
      2011-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] バブルリポソーム併用超音波遺伝子導入法を用いた間葉系幹細胞への遺伝子導入効果の解析2011

    • Author(s)
      鈴木健太郎, 根岸洋一, 高橋葉子, 高橋良太, 鈴木亮, 丸山一雄, 新槇幸彦
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report

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Published: 2010-11-30   Modified: 2019-07-29  

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