| Project/Area Number |
22650129
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MIURA Mikiko 島根大学, 医学部, 助教 (40447925)
AKIYAMA Haruhiko 京都大学, 医学研究科, 准教授 (60402830)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,140,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | 超音波 / 軟骨細胞 / 刺激 / 活性 / 超音波刺激 / 活性化 |
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| Research Abstract |
The effect of low-intensity pulsed ultrasound(LIPUS) on mRNA expression of matrix metalloproteinase-13(MMP13) of chondrocyte was investigated using real-time PCR method. Chondrocytes were isolated from articular cartilage in rat knee joints(Wistar, 12 week-old). After the chondrocytes were cultured, culture medium was changed for serum free or serum free with IL-1 beta, 100 pg/ml or 1 ng/ml concentration. Then, LIPUS was applied at 0, 7. 5, 30, 120 mW/cm2 intensity for 20-minutes. Total RNA was extracted immediately after 1-hour incubation. LIPUS could significantly inhibit the mRNA expression of MMP13 induced by IL-1 beta at 100 pg/ml concentration in intensity-dependent manner. In conclusion, LIPUS has a possibility to inhibit mRNA expression of MMP13 in intensity-dependent manner.
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