Establish a novel assay system to demonstrate the bioavailability of cancer preventive isothiocyanates in cruciferous vegetables by measuring its urinary metabolite

• Project/Area Number: 22650174
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kyoto Prefectural University
• Principal Investigator: NAKAMURA Yasushi 京都府立大学, 大学院・生命環境科学研究科, 准教授 (90285247)
• Co-Investigator(Kenkyū-buntansha): NISHIKAWA Akiyoshi 国立医薬品食品衛生研究所, 安全性生物試験研究センター, センター長 (30164544)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,000,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 生理活性 / 癌 / 生体機能利用 / 細菌 / 食品 / イソチオシアン酸 / 尿 / アブラナ科野菜 / 摂取基準値 / ダイコン / アブラナ科

Research Abstract

Our goal is to establish a novel assay system to demonstrate the bioavailability of isothiocyanate by measuring its urinary metabolite, dithiocarbamate. However conventional methods cannot discriminate between dithiocarbamates derived from other cruciferous vegetables vs. test vegetable, thus requiring subjects to fast from cruciferous vegetables during the test. To avoid the inconvenience of fasting from cruciferous vegetables, we developed a novel HPLC method that can fractionate dithiocarbamates in test vegetable from other dithiocarbamates. To validate the assay, we determined levels of urinary dithiocarbamate from human subjects fed with test vegetable-derived isothiocyanate. The time needed for assessment is several days, and does not require the inconvenience of the fasting.

Research Products

• [Journal Article] 京野菜の食品機能性における普及種に対する優位性とその利用
  • Author(s): 中村考志, 糸井昭太郎, 藤井雅弓, 中村貴子, 城田浩治, 末留昇, 曹永晩, 小川久美子, 西川秋佳, 松尾友明, 岡本繁久, 朴恩榮, 佐藤健司
  • Journal Title: 食品加工技術 Volume: (印刷中)
• [Presentation] ダイコンに含まれる抗変異原4-methylthio-3-butenyl isothiocyanateのハムスターでの生体内利用能 (2011)
  • Author(s): 藤井雅弓, 中村考志, 城田浩治, 末留昇, 西川秋佳, 小川久美子, 曹永晩, 朴恩榮, 佐藤健司
  • Organizer: 日本環境変異原学会
  • Place of Presentation: 学術総合センター(東京都)
  • Year and Date: 2011-11-21
• [Presentation] Antimutagenic and anticarcinogenic properties of Kyo-yasai, heirloom vegetables in Kyoto (2010)
  • Author(s): Nakamura Y, Shirota K, Suetome N, Matsuo T, Okamoto S, Nishikawa A, Imai T, Park EY, Sato K.
  • Organizer: 2nd Asian conference on environmental mutagens
  • Place of Presentation: Pataya, Thailand
  • Year and Date: 2010-12-17
• [Presentation] ダイコンに含まれる抗変異原4-methylthio-3-butenyl isothiocyanateのヒト生体内利用能の評価方法の確立 (2010)
  • Author(s): 糸井昭太郎, 中村考志, 城田浩治, 末留昇, 西川秋佳, 小川久美子, 曹永晩, 朴恩榮, 佐藤健司
  • Organizer: 日本環境変異原学会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2010-11-16