Development of a novel method for discovering fusion genes causative for tumorigenesis
| Project/Area Number |
22650234
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor diagnosis
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OKU Eijiro 久留米大学, 医学部, 助教 (10569429)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,530,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | 発現解析 / ゲノム / 癌 / バイオテクノロジー |
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| Research Abstract |
We developed a novel tool of gene analysis, based on a mate-pair analysis, to discover fusion genes that could be causative for tumorigenesis. In conventional mate-pair analysis, there must contain circularized DNAs derived from multiple DNAs and their mate-pair fragments can be a cause of false positive fusion genes. To solve this problem we developed a novel method which allows us to prepare circularized DNA libraries in which each circularized DNA is originated from each single DNA. By this method, we can make a systematic survey of the presence of fusion genes in cancers in a highly precise manner.
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Report
(3 results)
Research Products
(7 results)
