| Project/Area Number |
22651019
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Risk sciences of radiation/Chemicals
|
|---|
| Research Institution | Osaka Prefecture University |
|---|
Principal Investigator |
KODAMA Seiji 大阪府立大学, 大学院・理学系研究科, 教授 (00195744)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SHIRAISHI Kazunori 大阪府立大学, 大学院・理学系研究科, 助教 (40347513)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,490,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | 神経幹細胞 / 放射線生体影響 / ニューロスフェア / 不死化 / 2倍体細胞 / 2倍体 / DNA2本鎖切断 / リン酸化ヒストンH2AX / 未成熟染色体凝縮 |
|---|
| Research Abstract |
To establish an assay system for biological effect of radiation using neural stem cells, we used mouse neurosphere cells that contain neural stem cells, and fibroblast cells as a control. Neurosphere cells showed faster repair kinetics for DNA double strand breaks than fibroblast cells. To establish immortal neurosphere cell lines, we subcultured the neurosphere cells by successive transfer until the cells exceeded 100 population doubling numbers. Finally, we immortalized several mouse neurosphere cell lines that retained diploid karyotype and characteristics of neural stem cells.
|
