| Project/Area Number |
22651021
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,550,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | 突然変異 / SMRT DNAシーケンサー / 変異原性試験 / 次世代DNAシーケンサー / 遺伝毒性 / 変異原性 / SMRT |
|---|
| Research Abstract |
I proposed here an anticipated mutation assay which estimates the frequency and spectrum of somatic point mutations in any genes of any samples by using SMRT.(Single Molecule Real Time) DNA sequencing technology. The basic concept of the mutation assay is very simple : just prepare the target template and sequence it accurately. In this project, I considered ideas on how to make the template and how to eliminate artifactual mutations caused by damage to the DNA template. On the other hands, I developed a genome-wide mutation assay using Illumina next-generation DNA sequencer.
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