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Creation of biocatalyst capable of biosynthesizing lactate-based polymers with highly regulated chirality

Research Project

Project/Area Number 22651032
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Environmental technology/Environmental materials
Research InstitutionHokkaido University

Principal Investigator

TAGUCHI Seiichi  北海道大学, 大学院・工学研究院, 教授 (70216828)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,560,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywords生分解性プラスチック / 進化工学 / ポリヒドロキシアルカン酸 / キラリティー / 光学純度 / 乳酸ポリマー / 重合酵素 / モノマー供給系酵素 / キラルポリマー / 立体化学制御 / 酵素進化工学 / キラルモノマー / 微生物重合 / 基質特異性 / 進化分子工学 / ポリマー物性
Research Abstract

Currently microbial production system for lactate(LA) polymers with high performances has been developed. A break-through of this system was a discovery of "LA-Polymerizing Enzyme(LPE)". LPE was created through the study on the engineering of microbial polymer PHA synthase. LA-based polymer was synthesized by highly enatiomeric monomers including R-LA in an one-pot manner in Escherichia coli by introduction of the LPE gene from glucose. I tried to establish the system for synthesis of the polymer incorporating S-LA monomer based on the evolutionary engineering of CoA-transferase and LPE. As a resiult, LA fraction in the polymer has been enriched up to 47% from 6% by combination of metabolic and enzyme engineering. Furthermore, LA-based polymers exhibited distinguishable properties from the counterpart homopolymers, PLA and PHB.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (92 results)

All 2012 2011 2010 Other

All Journal Article (14 results) (of which Peer Reviewed: 10 results) Presentation (69 results) Book (6 results) Remarks (3 results)

  • [Journal Article] Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production2012

    • Author(s)
      Y. Song, K. Matsumoto, M. Yamada, A. Gohda, C. J. Brigham, A. J. Sinsky, S. Taguchi
    • Journal Title

      Appl. Microbiol. Biotechnol.

      Volume: 93 Pages: 1919-1925

    • Related Report
      2011 Final Research Report
  • [Journal Article] Biosynthesis of lactate(LA)-based polyester with a 96 mol% LA fraction and its application to stereocomplex formation2011

    • Author(s)
      F. Shozui, K. Matsumoto, R. Motohashi, J. Sun, S. Taguchi
    • Journal Title

      Polymer Degradation and Stability

      Volume: 96 Pages: 499-504

    • Related Report
      2011 Final Research Report
  • [Journal Article] Lactate fraction dependent mechanical properties of semitransparent poly(lactate-co-3-hydroxybutyrate) s produced by control of lactyl-CoA monomer fluxes in recombinant Escherichia coli2011

    • Author(s)
      M. Yamada, K. Matsumoto, S. Uramoto, R. Motohashi, H. Abe, S. Taguchi
    • Journal Title

      J. Biotechnol

      Volume: 154 Pages: 255-260

    • Related Report
      2011 Final Research Report
  • [Journal Article] Lactate fraction dependent mechanical properties of semitransparent poly(lactate-co-3-hydroxybutyrate)s produced by control of lactyl-CoA monomer fluxes in recombinant Escherichia coli2011

    • Author(s)
      M.Yamada, K.Matsumoto, S.Uramoto, R.Motohashi, H.Abe, S.Taguchi
    • Journal Title

      J.Biotechnol.

      Volume: 154(4) Issue: 4 Pages: 255-260

    • DOI

      10.1016/j.jbiotec.2011.05.011

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemo-enzymatic synthesis of polyhydroxyalkanoate (PHA) incorporating 2-hydroxybutyrate by wild-type class I PHA synthase from Ralstonia eutropha2011

    • Author(s)
      X.Han, Y.Satoh, T.Satoh, K.Matsumoto, T.Kakuchi, S.Taguchi, T.Dairi, M.Munekata, K.Tajima
    • Journal Title

      Appl.Microbiol.Biotechnol.

      Volume: 92(3) Issue: 3 Pages: 509-517

    • DOI

      10.1007/s00253-011-3362-8

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis of glycolate-based polyesters containing medium-chain-length 3-hydroxyalkanoates in recombinant Escherichia coli expressing engineered polyhydroxyalkanoate synthase2011

    • Author(s)
      K.Matsumoto, A.Ishiyama, K.Ssakai, T.Shiba, S.Taguchi
    • Journal Title

      J.Biotechnol.

      Volume: 156(3) Pages: 214-217

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance against 5-hydroxymethylfurfura 12011

    • Author(s)
      J.Masani Nduko, W.Suzuki, K.Matsumoto, H.Kobayashi, T.Ooi, A.Fukuoka, S.Taguchi
    • Journal Title

      J.Biosci.Bioeng.

      Volume: 113(1) Pages: 70-72

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemo-microbial conversion of cellulose into polyhydroxybutyrate through ruthenium-catalyzed hydrolysis of cellulose into glucose2011

    • Author(s)
      K.Matsumoto, et al.
    • Journal Title

      Bioresource Technology

      Volume: 102(3) Pages: 3564-3567

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis of lactate (LA)-based polyester with a 96 mol% LA fraction, its application to stereocomplex formation2011

    • Author(s)
      F.Shozui, et al.
    • Journal Title

      Polymer Degradation, Stability

      Volume: 96(4) Pages: 499-504

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new pathway for poly (3-hydroxybutyrate) production in Escherichia coli, Corynebacterium glutamicum by functional expression of a new acetoacetyl-coenzyme A synthase2011

    • Author(s)
      K.Matsumoto, et al.
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 75(2) Pages: 364-366

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of a metabolic pathway to introduce the 3-hydroxyhexanoate unit into LA-based polyesters via a reverse reaction ofβ-oxidation in Escherichia coli LS52182010

    • Author(s)
      F. Shozui, K. Matsumoto, R. Motohashi, M. Yamada, S. Taguchi
    • Journal Title

      Polymer Degradation and Stability

      Volume: 95 Pages: 340-1344

    • Related Report
      2011 Final Research Report
  • [Journal Article] Establishment of a metabolic pathway to introduce the 3-hydroxyhexanoate unit into LA-based polyesters via a reverse reaction of 6-oxidation in Escherichia coli LS52182010

    • Author(s)
      F.Shozui, et al.
    • Journal Title

      Polymer Degradation, Stability

      Volume: 95(8) Pages: 1340-1344

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A New Beneficial Mutation in Pseudomonas sp. 61-3 Polyhydroxyalkanoate (PHA) Synthase for Enhanced Cellular Content of 3-Hydroxybutyrate-Based PHA Explored Using Its Enzyme Homolog as a Mutation Template2010

    • Author(s)
      F.Shozui, et al.
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 74(8) Pages: 1710-1712

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of P (3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) terpolymers using a chimeric PHA synthase in recombinant Ralstonia eutropha, Pseudomonas putida2010

    • Author(s)
      J.Sun, et al.
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 74(8) Pages: 1716-1718

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Direct conversion of glucose into lactate-based polyesters using engineered Corynebacterium glutamicum as a whole cell catalyst2012

    • Author(s)
      Y.Song, K.Matsumoto, M.Yamada, A.Gohda, C.J.Brigham, A.J.Sinskey, S.Taguch
    • Organizer
      243rd ACS National Meeting
    • Place of Presentation
      San Diego Convention Center, (San Diego, USA)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Bioconversion of the hemicellulosic sugar, xylose, into lactate-based polyesters using recombinant Escherichia coli2012

    • Author(s)
      J.M.Nduko, K.Matsumoto, T.Ooi, S.Taguchi
    • Organizer
      243rd ACS National Meeting
    • Place of Presentation
      San Diego Convention Center, (San Diego, USA)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] 特殊環境下における微生物の潜在能力とその応用2012

    • Author(s)
      田口精一
    • Organizer
      日本農芸化学会2012年度(平成24年度)大会シンポジウム
    • Place of Presentation
      京都女子大学(京都市)(招待講演)
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] "乳酸バイオポリマー"生産用微生物工場の開発研究2012

    • Author(s)
      田口精一
    • Organizer
      2012年度産学官学術交流委員会フォーラム
    • Place of Presentation
      京都女子大学(京都市)(招待講演)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 進化工学による新規乳酸重合酵素の探索2012

    • Author(s)
      越智杏奈、大場貴史、坂井浩平、松本謙一郎、柘植丈治、田口精一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 組換え大腸菌における不飽和モノマーを導入した乳酸ベースポリマーの生合成2012

    • Author(s)
      孫健、正瑞文、松本謙一郎、田口精一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 組換え大腸菌による乳酸およびグリコール酸ベース新奇バイオプラスチックの生合成と物性解析2012

    • Author(s)
      斯波哲史、石山絢子、松本謙一郎、田口精一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Microbial Plastic Factory : from DNA to Materials2012

    • Author(s)
      S.Taguchi
    • Organizer
      International and Academia Cooperation Material and Chemical Research Laboratories
    • Place of Presentation
      Material and Chemical Research Laboratories, (Taiwan)(招待講演)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 「多元ポリ乳酸」創製のための合成生物学-試行錯誤のあれこれ-2012

    • Author(s)
      田口精一
    • Organizer
      セルロース学会北海道・東北支部セミナー
    • Place of Presentation
      北海道大学(札幌市)(招待講演)
    • Year and Date
      2012-02-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Biosynthesis of lactate-based polyester in food-grade bacterium2012

    • Author(s)
      Y. Song, K. Matsumoto, M. Yamada, A. Gohda, S. Taguchi
    • Organizer
      高分子学会北海道支部研究発表会
    • Place of Presentation
      札幌
    • Related Report
      2011 Final Research Report
  • [Presentation] A novel microbial production system for lactate-based polyester2012

    • Author(s)
      Y. Song, K. Matsumoto, M. Yamada, A. Gohda, S. Taguchi
    • Organizer
      The 5th GCOE International Symposium
    • Place of Presentation
      Hokkaido University, Sapporo
    • Related Report
      2011 Final Research Report
  • [Presentation] 進化工学による新規乳酸重合酵素の探索2012

    • Author(s)
      越智杏奈、大場貴史、坂井浩平、松本謙一郎、柘植丈治、田口精一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] 組換え大腸菌における不飽和モノマーを導入した乳酸ベースポリマーの生合成2012

    • Author(s)
      孫健、正瑞文、松本謙一郎、田口精一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] 組換え大腸菌による乳酸およびグリコール酸ベース新奇バイオプラスチックの生合成と物性解析2012

    • Author(s)
      斯波哲史、石山絢子、松本謙一郎、田口精一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] Direct conversion of glucose into lactate-based polyesters using engineered Corynebacterium glutamicum as a whole cell catalyst2012

    • Author(s)
      Y. Song, K. Matsumoto, M. Yamada, A. Gohda, C. J. Brigham, A. J. Sinskey, S. Taguchi
    • Organizer
      243rd ACS National Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Bioconversion of the hemicellulosic sugar, xylose, into lactate-based polyesters using recombinant Escherichia coli2012

    • Author(s)
      J. M. Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Organizer
      243rd ACS National Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] 「多元ポリ乳酸」創製のための合成生物学-試行錯誤のあれこれ-2012

    • Organizer
      セルロース学会北海道・東北支部セミナー
    • Place of Presentation
      北海道大学工学部
    • Related Report
      2011 Final Research Report
  • [Presentation] 乳酸バイオポリマー生産用微生物工場の開発研究2012

    • Organizer
      第7回農芸化学会研究企画賞受賞者最終報告会、2012年度産学官学術交流委員会フォーラム
    • Place of Presentation
      京都女子大学
    • Related Report
      2011 Final Research Report
  • [Presentation] Biosynthesis of lactate-based polyester in food-grade bacterium2012

    • Author(s)
      Y.Song, K.Matsumoto, M.Yamada, A.Gohda, S.Taguchi
    • Organizer
      高分子学会北海道支部研究発表会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] A novel microbial production system for lactate-based polyester2012

    • Author(s)
      Y.Song, K.Matsumoto, M.Yamada, A.Gohda, S.Taguchi
    • Organizer
      The 5th GCOE International Symposium
    • Place of Presentation
      Hokkaido University (Sapporo)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Microbial plastic factory : enzyme engineering and metabolic engineering2011

    • Author(s)
      S.Taguchi
    • Organizer
      「細胞を創る研究会」4
    • Place of Presentation
      千里ライフサイエンスセンター(豊中市)(招待講演)
    • Year and Date
      2011-10-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 微生物工場と植物工場によるバイオポリマー生産2011

    • Author(s)
      田口精一
    • Organizer
      BioJapan2011
    • Place of Presentation
      パシフィコ横浜(横浜市)(招待講演)
    • Year and Date
      2011-10-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] 微生物と植物によるポリエステルの合成:ポリ乳酸から多元ポリ乳酸へ2011

    • Author(s)
      田口精一
    • Organizer
      第8回よこはまバイオマス研究会講演会
    • Place of Presentation
      理研横浜研究所(横浜市)(招待講演)
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Biological Lactate-Polymers Synthesized by One-Pot Microbial Factory : How Different from Chemical Polylactate?2011

    • Author(s)
      S.Taguchi
    • Organizer
      ICMAT 2011- International Conference on Materials for Advanced Technology
    • Place of Presentation
      Pan Pacific Hotel, ( Suntec, Singapore)(招待講演)
    • Year and Date
      2011-06-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] 再生可能資源から高性能バイオポリマーを"一気通貫"合成する微生物工場の開発2011

    • Author(s)
      田口精一
    • Organizer
      JBA新資源生物変換研究会シンポジウム
    • Place of Presentation
      神戸大学百年記念館(神戸市)(招待講演)
    • Year and Date
      2011-06-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Biological lactate-polymers synthesized by one-pot microbial factory : Enzyme and metabolic engineering2011

    • Author(s)
      S.Taguchi
    • Organizer
      241th ACS National Meeting
    • Place of Presentation
      Anaheim Convention Center, Anaheim, CA, USA 招待講演
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Microbial Plastic Factory : from enzyme-metabolic engineering to polymer properties2011

    • Author(s)
      S.Taguchi
    • Organizer
      Technical Seminar on Bio-Technology in Japan, the Netherlands
    • Place of Presentation
      Kobe University, Kobe, Japan 招待講演
    • Year and Date
      2011-02-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Poly(lactate-co-3-hydroxybutyrate-co-3-hydroxyvalerate)の生合成および物性評価2011

    • Author(s)
      渡辺剛志、本橋廉、松本謙一郎、田口精一
    • Organizer
      第45回高分子学会北海道支部会研究発表会
    • Place of Presentation
      札幌
    • Related Report
      2011 Final Research Report
  • [Presentation] 乳酸ベース共重合体P(lactate-co-3-hydroxybutyrate-co-3-hydroxyvalerate)の生合成および物性評価2011

    • Author(s)
      孫健、本橋廉、正瑞文、松本謙一郎、田口精一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] 高乳酸分率のポリ(乳酸-co-3-ヒドロキシブタン酸)[ P(LA-co-3HB)]が合成可能な乳酸重合酵素の探索2011

    • Author(s)
      寺井彩月、正瑞文、松本謙一郎、田口精一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] Production of endotoxin-free lactate(LA)-based polyesters in recombinant Corynebacterium glutamicum2011

    • Author(s)
      Y. Song, A. Goda, K. Matsumoto, M. Yamada, S. Taguchi
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] ポリ(乳酸-co-3ヒドロキシブタン酸)[ P(LA-co-3HB)]のLA分率向上を目指したPseudomonassp. 61-3由来PHA重合酵素の変異体の探索2011

    • Author(s)
      寺井彩月、正瑞文、松本謙一郎、大井俊彦、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Biosynthesis of flexible lactate-based copolymers incorporating 3-hydroxyvalerate unit in recombinant Escherichia coli by feeding of propionate2011

    • Author(s)
      J. Sun, R. Motohashi, F. Shozui, K. Matsumoto, S. Taguchi
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] 組換え大腸菌による乳酸およびグリコール酸ベース新奇バイオプラスチックの生合成2011

    • Author(s)
      石山絢子,松本謙一郎,田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Biosynthesis of poly(lactate-co-3-hydroxybutyrate) in endotoxin-free Corynebacterium glutamicum expressing lactate-polymerizing enzyme2011

    • Author(s)
      Y. Song, A. Gohda, K. Matsumoto, M. Yamada, S. Taguchi
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Production of lactate-based polyesters from xylose in Escherichia coli2011

    • Author(s)
      J. M. Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Pseudomonas sp. SG4502由来耐熱性PHA合成酵素と乳酸重合酵素としての利用2011

    • Author(s)
      田島健次、韓雪容、佐藤康治、石井綾子、荒木祐治、棟方正信、田口精一
    • Organizer
      第60回高分子討論会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] Production of poly(lactate-co-3-hydroxybutyrate) in recombinant Corynebacterium glutamicum expressing lactate-polymerizing enzyme2011

    • Author(s)
      Y. Song, A. Gohda, K. Matsumoto, M. Yamada, S. Taguchi
    • Organizer
      2011 Taiwan-Japan Bilateral Polymer Symposium
    • Place of Presentation
      Taiwan
    • Related Report
      2011 Final Research Report
  • [Presentation] 高乳酸分率ポリ(乳酸-co-3-ヒドロキシブタン酸合成のための乳酸重合酵素の進化工学的改変2011

    • Author(s)
      坂井浩平、寺井彩月、正瑞文、越智杏奈、松本謙一郎、大井俊彦、田口精一
    • Organizer
      農芸化学会北海道支部学術講演会
    • Place of Presentation
      札幌
    • Related Report
      2011 Final Research Report
  • [Presentation] 乳酸およびグリコール酸ベース新奇バイオプラスチックの微生物生産2011

    • Author(s)
      斯波哲史、石山絢子、松本謙一郎、田口精一
    • Organizer
      農芸化学会北海道支部学術講演会
    • Place of Presentation
      札幌
    • Related Report
      2011 Final Research Report
  • [Presentation] Microbial Plastic Factory : from enzyme-metabolic engineering to polymer properties2011

    • Organizer
      Technical Seminar on Bio-Technology in Japan and the Netherlands
    • Place of Presentation
      Kobe University
    • Related Report
      2011 Final Research Report
  • [Presentation] Biological lactate-polymers synthesized by one-pot microbial factory : Enzyme and metabolic engineering2011

    • Organizer
      241st ACS National Meeting & Exposition
    • Place of Presentation
      Anaheim Convention Center & Area Hotels, Anaheim, California, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Biological Lactate-Polymers Synthesized by One-Pot Microbial Factory : How Different from Chemical Polylactate?2011

    • Organizer
      ICMAT 2011-International Conference on Materials for Advanced Technology
    • Place of Presentation
      Suntec Singapore and the Pan Pacific Singapore Hotel, Suntec, Singapore
    • Related Report
      2011 Final Research Report
  • [Presentation] 微生物と植物によるポリエステルの合成:ポリ乳酸から多元ポリ乳酸へ2011

    • Organizer
      第8回よこはまバイオマス研究会講演会
    • Place of Presentation
      理研横浜研究所
    • Related Report
      2011 Final Research Report
  • [Presentation] Advanced microbial polymer factory for incorporating new monomers : lactate, glycolate and 2-hydroxybutyrate2011

    • Author(s)
      K. Matsumoto, S. Taguchi
    • Organizer
      ICBP2011-The 3rd International Conference on Bio-based polymers 2011, the Meeting Hall of the College of Chemistry and Molecular Engineering
    • Place of Presentation
      Peking University. Peking University, Beijing, China
    • Related Report
      2011 Final Research Report
  • [Presentation] ポリ(乳酸-co-3ヒドロキシブタン酸)[P(LA-co-3HB)]のLA分率向上を目指したPseudomonas sp.61-3由来PHA重合酵素の変異体の探索2011

    • Author(s)
      寺井彩月、正瑞文、松本謙一郎、大井俊彦、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京農工大学(小金井市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 組換え大腸菌による乳酸およびグリコール酸ベース新奇バイオプラスチックの生合成2011

    • Author(s)
      石山絢子, 松本謙一郎, 田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京農工大学(小金井市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Biosynthesis of flexible lactate-based copolymers incorporating 3-hydroxy valerate unit in recombinant Escherichia coli by feeding of propionate2011

    • Author(s)
      J.Sun, R.Motohashi, F.Shozui, K.Matsumoto, S.Taguchi
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京農工大学(小金井市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Biosynthesis of poly(lactate-co-3-hydroxybutyrate) in endotoxin-free Cory nebacterium glutamicum expressing lactate-polymerizing enzyme2011

    • Author(s)
      Y.Song, A.Gohda, K.Matsumoto, M.Yamada, S.Taguchi
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京農工大学(小金井市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Production of lactate-based polyesters from xylose in Escherichia coli2011

    • Author(s)
      J.M.Nduko, K.Matsumoto, T.Ooi, S.Taguchi
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京農工大学(小金井市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Production of poly(lactate-co-3-hydroxybutyrate) in recombinant Coryneb acterium glutamicum expressing lactate-polymerizing enzyme2011

    • Author(s)
      Y.Song, A.Gohda, K.Matsumoto, M.Yamada, S.Taguchi
    • Organizer
      2011 Taiwan-Japan Bilateral Polymer Symposium
    • Place of Presentation
      National Chiao Tung University, (Taiwan)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Advanced microbial polymer factory for incorporating new monomers : lactate, glycolate and 2-hydroxybutyrate2011

    • Author(s)
      K.Matsumoto, S.Taguchi
    • Organizer
      The 3rd International Conference on Bio-based polymers 2011
    • Place of Presentation
      Peking University, ( Beijing, China)(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 高乳酸分率ポリ(乳酸-co-3-ヒドロキシブタン酸合成のための乳酸重合酵素の進化工学的改変2011

    • Author(s)
      坂井浩平、寺井彩月、正瑞文、越智杏奈、松本謙一郎、大井俊彦、田口精一
    • Organizer
      農芸化学会北海道支部学術講演会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Advanced studies on microbial lactate-based polymers : enzyme-metabolic engineering, polymer properties2010

    • Author(s)
      S.Taguchi
    • Organizer
      ISBP 2010 - International Symposium on Biopolymers
    • Place of Presentation
      Haus der Wirtschaft Baden-Wurttemberg, Stuttgart, Germany 招待講演
    • Year and Date
      2010-10-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] バイオマス・リファイナリー原料から微生物合成する硬軟設計可能なバイオポリマー2010

    • Author(s)
      田口精一
    • Organizer
      財団法人化学技術戦略推進機構バイオマス利活用研究会
    • Place of Presentation
      東京堂神保町第一ビル6階会議室東京 招待講演
    • Year and Date
      2010-08-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] グリーンイノベーション:バイオポリマー生産のための微生物工場・植物工場の開発2010

    • Author(s)
      田口精一
    • Organizer
      日本化学会群馬地区講演会
    • Place of Presentation
      群馬大学大学院工学研究科総合研究棟、桐生 招待講演
    • Year and Date
      2010-06-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 新奇かつデザイナブルなバイオポリエステル合成のための重合酵素の機能改変2010

    • Author(s)
      松本謙一郎
    • Organizer
      第10回日本蛋白質科学会年会、ワークショップ
    • Place of Presentation
      札幌コンベンションセンター札幌 招待講演
    • Year and Date
      2010-06-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] The microbial factory for synthesis of lactate-based polyetser by using lactate- polymerizing enzyme2010

    • Author(s)
      S.Taguchi
    • Organizer
      Finland-Japan Biotechnology Symposium 2010
    • Place of Presentation
      Turku University, Turku, Finland 招待講演
    • Year and Date
      2010-06-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] バイオベースポリマー生産のための微生物工場と植物工場2010

    • Author(s)
      田口精一
    • Organizer
      第24回環境工学連合講演会:環境保全のための新技術開発
    • Place of Presentation
      日本学術会議講堂東京 招待講演
    • Year and Date
      2010-04-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Microbial Production of Lactate-based Polyesters2010

    • Author(s)
      K. Matsumoto, M. Yamada, F. Shozui, S. Uramoto, R. Motohashi T. Nakai, K. Shimizu, S. Taguchi
    • Organizer
      第59回高分子学会年次大会
    • Place of Presentation
      横浜
    • Related Report
      2011 Final Research Report
  • [Presentation] ジャーファーメンターを用いた乳酸ポリマーの微生物発酵生産と物性評価2010

    • Author(s)
      本橋廉、浦本周、山田美和、正瑞文、松本謙一郎、田口精一
    • Organizer
      農芸化学会北海道支部学術講演会
    • Place of Presentation
      札幌
    • Related Report
      2011 Final Research Report
  • [Presentation] 組換え大腸菌を用いたポリ(乳酸-co-3-ヒドロキシブタン酸-co-3-ヒドロキシヘキサン酸)の生産2010

    • Author(s)
      正瑞文、本橋廉、山田美和、松本謙一郎、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      宮崎
    • Related Report
      2011 Final Research Report
  • [Presentation] Microbial production of lactate(LA)-based polyesters with tailor-made monomer composition using engineered LA-polymerizing enzymes2010

    • Author(s)
      M. Yamada, K. Matsumoto, T. Nakai, K. Shimizu, S. Uramoto, F. Shozui, S. Taguchi
    • Organizer
      2010 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Honolulu
    • Related Report
      2011 Final Research Report
  • [Presentation] The microbial factory for synthesis of lactate-based polyetser by using lactatepolymerizing enzyme2010

    • Organizer
      Finland-Japan Biotechnology Symposium 2010
    • Place of Presentation
      Turku, Finland
    • Related Report
      2011 Final Research Report
  • [Presentation] 新奇かつデザイナブルなバイオポリエステル合成のための重合酵素の機能改変2010

    • Author(s)
      松本謙一郎、田口精一
    • Organizer
      第10回日本蛋白質科学会年会、ワークショップ
    • Place of Presentation
      札幌コンベンションセンター
    • Related Report
      2011 Final Research Report
  • [Presentation] Advanced studies on microbial lactate-based polymers : enzyme-metabolic engineering and polymer properties2010

    • Organizer
      ISBP 2010-International Symposium on Biopolymers, Haus der Wirtschaft, Stuttgart
    • Place of Presentation
      Germany(Plenary Lecture)
    • Related Report
      2011 Final Research Report
  • [Presentation] New mutations in lactate(LA)-polymerizing enzyme for the microbial production of LA-based polyesters with tailor-made monomer composition2010

    • Author(s)
      M. Yamada, T. Nakai, K. Shimizu, S. Uramoto, F. Shozui, K. Matsumoto, S. Taguchi
    • Organizer
      ISBP 2010-International Symposium on Biopolymers, Haus der Wirtschaft, Stuttgart
    • Place of Presentation
      Germany
    • Related Report
      2011 Final Research Report
  • [Presentation] Construction of a metabolic pathway to introduce the 3-hydroxyhexanoate unit into LA-based polyesters via a reverse reaction ofβ-oxidation in Escherichia coli LS52182010

    • Author(s)
      F. Shozui, K. Matsumoto, R. Motohashi, M. Yamada, S. Taguchi
    • Organizer
      ISBP 2010-International Symposium on Biopolymers, Haus der Wirtschaft, Stuttgart
    • Place of Presentation
      Germany
    • Related Report
      2011 Final Research Report
  • [Presentation] Current advances in microbial cell factories for lactate-based polyesters driven by lactate-polymerizing enzymes2010

    • Organizer
      Pacifichem 2010, Biodegradable and Biomass Plastics
    • Place of Presentation
      Convention Center, Honolulu, Hawaii, USA
    • Related Report
      2011 Final Research Report
  • [Book] 合成生物工学の隆起-有用物質の新たな生産法構築をめざして-2012

    • Author(s)
      渡辺剛志、越智杏奈、田口精一
    • Publisher
      シーエムシー出版社
    • Related Report
      2011 Annual Research Report
  • [Book] 第1章新しい植物由来ポリマー・材料の開発: 9節微生物を用いた乳酸ポリマーのワンステップ重合法2011

    • Author(s)
      越智杏奈、渡辺剛志、田口精一
    • Related Report
      2011 Final Research Report
  • [Book] 植物由来ポリマー・複合材料の開発2011

    • Author(s)
      越智杏奈、渡辺剛志、田口精一
    • Publisher
      サイエンス&テクノロジー株式会社
    • Related Report
      2011 Annual Research Report
  • [Book] 乳酸ポリマーのワンポット微生物合成2010

    • Author(s)
      田口精一
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Final Research Report
  • [Book] 酵素利用技術大系2010

    • Author(s)
      田口精一
    • Total Pages
      1064
    • Publisher
      エヌ・ティ・エス出版
    • Related Report
      2010 Annual Research Report
  • [Book] エコバイオリファイナリー-脱石油社会へ移行するための環境ものづくり戦略-2010

    • Author(s)
      田口精一
    • Total Pages
      252
    • Publisher
      シーエムシー出版
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.eng.hokudai.ac.jp/labo/seika/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.eng.hokudai.ac.jp/labo/seika/TOP.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.eng.hokudai.ac.jp/labo/seika/contents/article/article.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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