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Construction of label-free biochips using anomalous reflection of gold

Research Project

Project/Area Number 22651047
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Nanomaterials/Nanobioscience
Research InstitutionTokyo Institute of Technology

Principal Investigator

MIHARA Hisakazu  東京工業大学, 大学院・生命理工学研究科, 教授 (30183966)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,530,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsバイオチップ / 金基板 / 反射法 / 非標識 / ペプチド
Research Abstract

A efficient label-free detection method is desired to improve the technology of biochips including protein microarray. In this study, the novel label-free detection method using anomalous reflection of gold(AR) was developed. The AR method is highly sensitive and easy to miniaturize so as to apply it to biochips detecting various peptide and protein interactions in a high-throughput manner.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (11 results)

All 2012 2011 2010 Other

All Journal Article (5 results) (of which Peer Reviewed: 2 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] Sensitive Detection of Small Molecule-Protein Interactions on a Metal-Insulator-Metal Label-Free Biosensing Platform2012

    • Author(s)
      A. Syahir, K. Kajikawa, H. Mihara
    • Journal Title

      Chem. Asia. J.

      Volume: (in press)

    • Related Report
      2011 Final Research Report
  • [Journal Article] A New Optical Label-Free Biosensing Platform Based on a Metal. Insulator. Metal Structure2010

    • Author(s)
      A. Syahir, H. Mihara, K. Kajikawa
    • Journal Title

      Langmuir

      Volume: 26 Pages: 6053-6057

    • Related Report
      2011 Final Research Report
  • [Journal Article] A New Optical Label-Free Biosensing Platform Based on a Metal-Insulator-Metal Structure2010

    • Author(s)
      Amir Syahir, Hisakazu Mihara, Kotaro Kajikawa
    • Journal Title

      Langmuir

      Volume: 26 Pages: 6053-6057

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] オミクス研究に向けた設計ペプチドアレイの構築

    • Author(s)
      臼井健二、富崎欣也、三原久和
    • Journal Title

      分析化学

      Volume: (印刷中)

    • NAID

      10030436925

    • Related Report
      2011 Final Research Report
  • [Journal Article] "オミクズ"研究に向けた設計ペプチドアレイの構築

    • Author(s)
      臼井健二, 富崎欣也, 三原久和
    • Journal Title

      分析化学

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 金の異常反射法による金基板上でのプロテアーゼ反応の検出2011

    • Author(s)
      田島健一、アミルシャヒル、梶川浩太郎、三原久和
    • Organizer
      第48回ペプチド討論会
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 金の異常反射を利用したプロテアーゼ反応の検出2011

    • Author(s)
      田島健一、Amir Syahir、梶川浩太郎、三原久和
    • Organizer
      第91日本化学会春季年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Final Research Report
  • [Presentation] 金の異常反射(AR)法を利用したプロテアーゼ反応の検出2011

    • Author(s)
      田島健一
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2011 Annual Research Report
  • [Presentation] A designed peptide library for protein analyses2010

    • Author(s)
      Hisakazu Mihara
    • Organizer
      14^<th> Korean Peptide and Protein Symposium
    • Place of Presentation
      ソウル
    • Year and Date
      2010-12-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Designed peptide libraries for protein and cell analyses2010

    • Author(s)
      Hisakazu Mihara
    • Organizer
      5thInternational Peptide Symposium/47thJapanese Peptide Symposium
    • Place of Presentation
      Kyoto
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.mihara.bio.titech.ac.jp

    • Related Report
      2011 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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