| Project/Area Number |
22651048
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
HISABORI Toru 東京工業大学, 資源化学研究所, 教授 (40181094)
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| Co-Investigator(Renkei-kenkyūsha) |
MASUDA Shinji 東京工業大学, 大学院・生命理工学研究科, 准教授 (30373369)
IKEUCHI Masahiko 東京大学, 大学院・総合文化研究科, 教授 (20159601)
TAKEUCHI Shoji 東京大学, 生産技術研究所, 准教授 (90343110)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,550,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | 分子マニピュレーション / 光スイッチ / 酸化還元 / ATP合成酵素 / 分子モーター / レドックス酵素 / 酸化還元制御 |
|---|
| Research Abstract |
Based on our hypothesis that the activity of ATP synthase can be regulated by the relative slippage of two alpha-helices of the gamma subunit, we intended to restrict the movement of two alpha-helices and studied the change in the enzyme activity. Then we tried to introduce a novel artificial switch into the gamma subunit to control the enzyme activity using a certain domain of light-responsible protein. In addition, the highly sensitive monitoring system to measure the enzyme activity at single molecule level at the aqueous phase was developed.
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