| Project/Area Number |
22651083
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | ケミカルバイオロジー / 糖鎖 / 糖鎖プライマー / がん細胞 / がんの転移性 / 糖鎖合成遺伝子 / 質量分析 / 糖鎖生合成経路 |
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| Research Abstract |
The development of "comparative glycomics" to analyze the glycans in cells and to compare the glycans between the cells having different metastasis using saccharide primer method is the purpose of this study. The major products obtained by saccharide primer methods from highly metastatic human A549 lung adenocarcinoma cells were sulfonated glycans. Knockdown of the sulfotransferase genes using siRNA showed the suppression of cell motility, and it was indicated that sulfonated glycans are related with the metastasis of lung carcinoma cells. The major products obtained by saccharide primer methods from low metastatic murine FBJ-S1 osteosarcoma cells compared to highly metastatic FBJ-LL cells were gangliosides such as GD1a and glycosaminoglycans such as heparan sulfate. It was indicated that the expression of heparan sulfate suppressed the metastasis of osteosarcoma cells. In this study, it was shown that saccharide primer method was useful to analyze the glycans related with the metastasis of tumor cells. And the new molecular mechanisms for tumor metastases were found by saccharide primer method.
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