Creation of First Enzyme-Type Zinc Finger Protein PossessingCatalytic Function

• Project/Area Number: 22651085
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Doshisha Women's College of Liberal Arts
• Principal Investigator: SUGIURA Yukio 同志社女子大学, 薬学部, 教授 (40025698)
• Co-Investigator(Kenkyū-buntansha): NEGI Shigeru 同志社女子大学, 薬学部, 助教 (50378866)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 亜鉛フィンガー / DNA 認識 / DNA 切断 / 触媒機能 / 酵素類似 / 分子設計 / 加水分解 / 機能変換 / 人工設計 / DNA認識 / DNA切断 / 転写因子 / 制限酵素

Research Abstract

On the basis of native zinc finger protein, design and creation of new zinc finger protein possessing catalytic function were performed. Artificial His4-type zinc finger protein was demonstrated to have DNA hydrolytic function. We firstly succeeded in creation of new enzyme-type zinc finger protein.

Research Products

• [Journal Article] Rational design of DNA sequencespecific zinc fingers (2012)
  • Author(s): H.Kono, M.Imanishi, S.Negi, K.Tatsutani, T.Sakaeda, A.Hashimoto, C.Nakayama, S.Futaki, Y.Sugiura
  • Journal Title: FEBS Lett Volume: 586(6) Pages: 918-923
  • Peer Reviewed
• [Journal Article] New functions of zinc fingers revealed by substitution of conserved residues, Zinc Fingers:Struc-ture, Properties and Functions, Ed. By S.Gonzalez (2012)
  • Author(s): M.Imanishi, Y.Sugiura
  • Journal Title: NovaScience Publishers Pages: 141-154
  • Peer Reviewed
• [Journal Article] Zn(II) binding and DNA binding properties of ligand-substituted XXHH-type zinc finger proteins (2012)
  • Author(s): M.Imanishi, K.Matsumura, S.Tsuji, T.Nakaya, S.Negi, S.Futaki, Y.Sugiura
  • Journal Title: Biochemistry Volume: 51(16) Pages: 3342-3348
  • Peer Reviewed
• [Journal Article] Zn(II) binding and DNA binding properties of ligand-substituted CXHH-type zinc finger proteins (2012)
  • Author(s): M.Imanishi
  • Journal Title: Biochemistry Volume: 51 Issue: 16 Pages: 3342-3348
  • DOI: 10.1021/bi300236m
  • Peer Reviewed
• [Journal Article] An arginine residue instead of a conserved leucine residue in the recognition helix of the finger 3 of Zif268 stabilizes the domein structure and mediates DNA binding (2011)
  • Author(s): S.Negi, M.Imanishi, M.Sasaki, K.Tatsutani, S.Futaki, Y.Sugiura
  • Journal Title: Biochemistry Volume: 50(28) Pages: 6366-6372
  • Peer Reviewed
• [Journal Article] An arginine residue instead of a conserved leucine residue in the recognition helix of the finger 3 of Zif268 stabilizes the domain structure and mediates DNA binding (2011)
  • Author(s): S.Negi
  • Journal Title: Biochemistry Volume: 50巻 Issue: 28 Pages: 6366-6372
  • DOI: 10.1021/bi200697p
  • Peer Reviewed
• [Journal Article] Zinc finger-zinc finger interaction between the transcription factors, GATA-1 and Sp1 (2010)
  • Author(s): M.Imanishi, C.Imamura, C.Hogashi, W.Yan, S.Negi, S.Futaki, Y.Sugiura
  • Journal Title: Biochem.Biophys.Res.Commun Volume: 400(4) Pages: 625-630
  • Peer Reviewed
• [Journal Article] Metal-stimulated transcriptional regulation by an artificial zinc fingerprotein (2010)
  • Author(s): M.Imanishi, T.Nakaya, T.Morisaki, D.Noshiro, S.Futaki, Y.Sugiura
  • Journal Title: ChemBioChem Volume: 11(12) Pages: 1653-1655
  • Peer Reviewed
• [Journal Article] Zinc finger-zinc finger interaction between the transcription factors, GATA-1 and Spl (2010)
  • Author(s): M.Imanishi, C.Imamura, C.Higashi, W.Yan, S.Negi, S.Futaki, Y.Sugiura
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 400(4) Pages: 625-630
  • Peer Reviewed
• [Journal Article] Non-FolkI-based zinc finger nucleases, Engineered Zinc Finger Proteins, Ed. by J.Markay & D.Segal (2010)
  • Author(s): M.Imanishi, S.Negi, Y.Sugiura
  • Journal Title: Methods in Molecular Biology Pages: 337-349
  • Peer Reviewed
• [Journal Article] Metal-stimulated transcriptional regulation by an artificial zinc finger protein (2010)
  • Author(s): M,Imanishi
  • Journal Title: ChemBioChem. Volume: 11 Pages: 1653-1655
  • Peer Reviewed
• [Presentation] 亜鉛フィンガータンパク質のレドックス挙動 (2012)
  • Author(s): 根木滋、山岡槇子、福田あずさ、杉浦幸雄
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 横浜市
  • Year and Date: 2012-03-25
• [Presentation] GAGA metallofinger peptide: Its metal-binding, DNA-binding and redox properties (2012)
  • Author(s): 根木 滋
  • Organizer: 6th ABICC
  • Place of Presentation: Hong Kong
• [Presentation] 人工亜鉛フィンガータンパク質のデザインおよびその機能 (2011)
  • Author(s): 根木滋、谷口仁哉、杉浦幸雄
  • Organizer: 第36回生体分子科学討論会
  • Place of Presentation: 札幌市
  • Year and Date: 2011-06-19
• [Presentation] 配位子置換亜鉛フィンガータンパク質のDNA結合特性 (2011)
  • Author(s): 今西未来、中尾智博、松林一央、二木史朗、杉浦幸雄
  • Organizer: 第24回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 千葉市
  • Year and Date: 2011-05-31
• [Presentation] GAGA亜鉛フィンガータンパク質の酸化に伴う構造および機能変化 (2011)
  • Author(s): 根木滋、増山紗永子、山岡槇子、杉浦幸雄
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-03-26
• [Presentation] 新規GAGA金属置換型フィンガーの創製およびその機能評価 (2011)
  • Author(s): 増山紗永子、根木滋、野口範子、杉浦幸雄
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-03-26
• [Presentation] リデザインによる野生型亜鉛フィンガー蛋白質の機能変換 (2010)
  • Author(s): 根木滋、三間紘子、杉浦幸雄
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌市
  • Year and Date: 2010-06-18
• [Presentation] リデザインによる野生型亜鉛フィンガータンパク質の機能変換 (2010)
  • Author(s): 根木滋
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌市
  • Year and Date: 2010-06-18