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Creation of First Enzyme-Type Zinc Finger Protein PossessingCatalytic Function

Research Project

Project/Area Number 22651085
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Living organism molecular science
Research InstitutionDoshisha Women's College of Liberal Arts

Principal Investigator

SUGIURA Yukio  同志社女子大学, 薬学部, 教授 (40025698)

Co-Investigator(Kenkyū-buntansha) NEGI Shigeru  同志社女子大学, 薬学部, 助教 (50378866)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywords亜鉛フィンガー / DNA 認識 / DNA 切断 / 触媒機能 / 酵素類似 / 分子設計 / 加水分解 / 機能変換 / 人工設計 / DNA認識 / DNA切断 / 転写因子 / 制限酵素
Research Abstract

On the basis of native zinc finger protein, design and creation of new zinc finger protein possessing catalytic function were performed. Artificial His4-type zinc finger protein was demonstrated to have DNA hydrolytic function. We firstly succeeded in creation of new enzyme-type zinc finger protein.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (19 results)

All 2012 2011 2010

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (8 results)

  • [Journal Article] Rational design of DNA sequencespecific zinc fingers2012

    • Author(s)
      H.Kono, M.Imanishi, S.Negi, K.Tatsutani, T.Sakaeda, A.Hashimoto, C.Nakayama, S.Futaki, Y.Sugiura
    • Journal Title

      FEBS Lett

      Volume: 586(6) Pages: 918-923

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] New functions of zinc fingers revealed by substitution of conserved residues, Zinc Fingers:Struc-ture, Properties and Functions, Ed. By S.Gonzalez2012

    • Author(s)
      M.Imanishi, Y.Sugiura
    • Journal Title

      NovaScience Publishers

      Pages: 141-154

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Zn(II) binding and DNA binding properties of ligand-substituted XXHH-type zinc finger proteins2012

    • Author(s)
      M.Imanishi, K.Matsumura, S.Tsuji, T.Nakaya, S.Negi, S.Futaki, Y.Sugiura
    • Journal Title

      Biochemistry

      Volume: 51(16) Pages: 3342-3348

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Zn(II) binding and DNA binding properties of ligand-substituted CXHH-type zinc finger proteins2012

    • Author(s)
      M.Imanishi
    • Journal Title

      Biochemistry

      Volume: 51 Issue: 16 Pages: 3342-3348

    • DOI

      10.1021/bi300236m

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An arginine residue instead of a conserved leucine residue in the recognition helix of the finger 3 of Zif268 stabilizes the domein structure and mediates DNA binding2011

    • Author(s)
      S.Negi, M.Imanishi, M.Sasaki, K.Tatsutani, S.Futaki, Y.Sugiura
    • Journal Title

      Biochemistry

      Volume: 50(28) Pages: 6366-6372

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] An arginine residue instead of a conserved leucine residue in the recognition helix of the finger 3 of Zif268 stabilizes the domain structure and mediates DNA binding2011

    • Author(s)
      S.Negi
    • Journal Title

      Biochemistry

      Volume: 50巻 Issue: 28 Pages: 6366-6372

    • DOI

      10.1021/bi200697p

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Zinc finger-zinc finger interaction between the transcription factors, GATA-1 and Sp12010

    • Author(s)
      M.Imanishi, C.Imamura, C.Hogashi, W.Yan, S.Negi, S.Futaki, Y.Sugiura
    • Journal Title

      Biochem.Biophys.Res.Commun

      Volume: 400(4) Pages: 625-630

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metal-stimulated transcriptional regulation by an artificial zinc fingerprotein2010

    • Author(s)
      M.Imanishi, T.Nakaya, T.Morisaki, D.Noshiro, S.Futaki, Y.Sugiura
    • Journal Title

      ChemBioChem

      Volume: 11(12) Pages: 1653-1655

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Zinc finger-zinc finger interaction between the transcription factors, GATA-1 and Spl2010

    • Author(s)
      M.Imanishi, C.Imamura, C.Higashi, W.Yan, S.Negi, S.Futaki, Y.Sugiura
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 400(4) Pages: 625-630

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Non-FolkI-based zinc finger nucleases, Engineered Zinc Finger Proteins, Ed. by J.Markay & D.Segal2010

    • Author(s)
      M.Imanishi, S.Negi, Y.Sugiura
    • Journal Title

      Methods in Molecular Biology

      Pages: 337-349

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metal-stimulated transcriptional regulation by an artificial zinc finger protein2010

    • Author(s)
      M,Imanishi
    • Journal Title

      ChemBioChem.

      Volume: 11 Pages: 1653-1655

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 亜鉛フィンガータンパク質のレドックス挙動2012

    • Author(s)
      根木滋、山岡槇子、福田あずさ、杉浦幸雄
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      横浜市
    • Year and Date
      2012-03-25
    • Related Report
      2012 Final Research Report
  • [Presentation] GAGA metallofinger peptide: Its metal-binding, DNA-binding and redox properties2012

    • Author(s)
      根木 滋
    • Organizer
      6th ABICC
    • Place of Presentation
      Hong Kong
    • Related Report
      2012 Annual Research Report
  • [Presentation] 人工亜鉛フィンガータンパク質のデザインおよびその機能2011

    • Author(s)
      根木滋、谷口仁哉、杉浦幸雄
    • Organizer
      第36回生体分子科学討論会
    • Place of Presentation
      札幌市
    • Year and Date
      2011-06-19
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 配位子置換亜鉛フィンガータンパク質のDNA結合特性2011

    • Author(s)
      今西未来、中尾智博、松林一央、二木史朗、杉浦幸雄
    • Organizer
      第24回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      千葉市
    • Year and Date
      2011-05-31
    • Related Report
      2012 Final Research Report
  • [Presentation] GAGA亜鉛フィンガータンパク質の酸化に伴う構造および機能変化2011

    • Author(s)
      根木滋、増山紗永子、山岡槇子、杉浦幸雄
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-03-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 新規GAGA金属置換型フィンガーの創製およびその機能評価2011

    • Author(s)
      増山紗永子、根木滋、野口範子、杉浦幸雄
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-03-26
    • Related Report
      2012 Final Research Report
  • [Presentation] リデザインによる野生型亜鉛フィンガー蛋白質の機能変換2010

    • Author(s)
      根木滋、三間紘子、杉浦幸雄
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌市
    • Year and Date
      2010-06-18
    • Related Report
      2012 Final Research Report
  • [Presentation] リデザインによる野生型亜鉛フィンガータンパク質の機能変換2010

    • Author(s)
      根木滋
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌市
    • Year and Date
      2010-06-18
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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