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Development of High Performance Gene Transfection Methods Using Microplasma Integrated Devices

Research Project

Project/Area Number 22654070
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Plasma science
Research InstitutionOsaka Electro-Communication University (2011)
Ehime University (2010)

Principal Investigator

TACHIBANA Kunihide  大阪電気通信大学, 工学部, 教授 (40027925)

Co-Investigator(Kenkyū-buntansha) JINNO Masafumi  愛媛大学, 理工学研究科, 教授 (30274335)
MOTOMURA Hideki  愛媛大学, 理工学研究科, 助教 (80332831)
Co-Investigator(Renkei-kenkyūsha) HASHIMOTO Koji  愛媛大学, 医学研究科, 教授 (00110784)
SHIRAKATA Yuji  愛媛大学, 医学研究科, 講師 (50226320)
JINNO Masafumi  愛媛大学, 理工学研究科, 教授 (30274335)
MOTOMURA Hideki  愛媛大学, 理工学研究科, 助教 (80332831)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥2,950,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywords反応性プラズマ / マイクロプラズマ / プラズマメディシン / 遺伝子導入 / プラズマジェット / エレクトロスプレー / GFP遺伝子 / COS細胞
Research Abstract

We have tried gene transfection experiments using an integrated device composed of microplasma jets. After the irradiation of plasma jet onto COS cells mixed with GFP genes and cultivation of those cells for 24 hours, the GFP genes have been successfully transfected into a certain amount of cells. Through a series of experiments with several kinds of plasma sources, it is concluded that the effects of transported electric charges from plasma produced particles play a key role in the transfection mechanisms. Upon this understanding, we have developed a new electro-spray method in which a higher efficiency of transfection is achieved with suppressing the damages on living cells.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (13 results)

All 2012 2011

All Presentation (11 results) Patent(Industrial Property Rights) (2 results)

  • [Presentation]2012

    • Author(s)
      H. Motomura, J. Matsuda, K. Ikeda, T. Okihiro, M. Jinno, K. Tachibana, S. Satoh, N. Saeki
    • Organizer
      4th International Conference on Plasma Medicine
    • Place of Presentation
      Orleans, France
    • Year and Date
      2012-06-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Validation of Plasma Irradiation Effect on Gene Transfection by Using Microplasma Jet from Capillary Nozzle2012

    • Author(s)
      T. Okihiro, J. Matsuda, K. Ikeda, H. Motomura, M. Jinno, K. Tachibana, S. Satoh, N. Saeki
    • Organizer
      4th International Conference on Plasma Medicine
    • Place of Presentation
      Orleans, France
    • Year and Date
      2012-06-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Gene Transfection by Electric Charge Injection Using Electrospray2012

    • Author(s)
      K.Tachibana
    • Organizer
      4th International Conference on Plasma Medicine
    • Place of Presentation
      Orleans, France
    • Year and Date
      2012-06-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Validation of Plasma Irradiation Effect on Gene Transfection by Using Microplasma Jet2012

    • Author(s)
      K.Tachibana
    • Organizer
      4th International Conference on Plasma Medicine
    • Place of Presentation
      Orleans, France
    • Year and Date
      2012-06-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Quantitative Investigation of Energy Injection and Gas Flow Rate for the Plasma Used in Gene Transfection2012

    • Author(s)
      K.Tachibana
    • Organizer
      4th International Conference on Plasma Medicine
    • Place of Presentation
      Orleans, France
    • Year and Date
      2012-06-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] プラズマ遺伝子導入法における電荷の効果の検証2012

    • Author(s)
      沖廣仁、池田健太郎、松田淳、本村英樹、神野雅文、橘邦英、佐藤晋、佐伯登
    • Organizer
      応用物理学会
    • Place of Presentation
      東京
    • Year and Date
      2012-03-16
    • Related Report
      2011 Final Research Report
  • [Presentation] プラズマ遺伝子導入法における電荷の効果の検証2012

    • Author(s)
      橘邦英
    • Organizer
      第59回応用物理学関係連合講演会
    • Place of Presentation
      早稲田大学(東京都)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 遺伝子導入のためのマイクロプラズマジェットの最適化2011

    • Author(s)
      沖廣仁、池田健太郎、松田淳、本村英樹、神野雅文、橘邦英、佐藤晋、佐伯登
    • Organizer
      応用物理学会
    • Place of Presentation
      山形
    • Year and Date
      2011-08-31
    • Related Report
      2011 Final Research Report
  • [Presentation] 遺伝子導入のためのマイクロプラズマジェットの最適化2011

    • Author(s)
      橘邦英
    • Organizer
      第72回応用物理学会学術講演会
    • Place of Presentation
      山形大学(山形市)
    • Year and Date
      2011-08-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] A comparative study on bullet-like discharge characteristics in atmospheric-pressure microplasma jet between argon and helium working gases2011

    • Author(s)
      K.Tachibana, H.Motomura, M.Jinno, et al.
    • Organizer
      6^<th> International Workshop on Microplasmas
    • Place of Presentation
      Paris, France
    • Year and Date
      2011-04-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] マイクロプラズマジェットによる遺伝子導入2011

    • Author(s)
      橘邦英, 神野雅文, 本村英樹, 他
    • Organizer
      第58回応用物理学関係連合講演会
    • Place of Presentation
      厚木市
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 標的細胞への選定分子の導入法およびそれに用いる選定分子導入装置(エレクトロスプレー法)2012

    • Inventor(s)
      神野雅文、本村英樹、橘邦英、沖廣仁、佐藤晋、佐伯登
    • Industrial Property Rights Holder
      神野雅文、本村英樹、橘邦英、沖廣仁、佐藤晋、佐伯登
    • Industrial Property Number
      2012-134989
    • Filing Date
      2012-06-14
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] 標的細胞への選定分子の導入法およびそれに用いる選定分子導入装置(マイクロキャピラリープラズマ法)2012

    • Inventor(s)
      神野雅文、本村英樹、橘邦英、沖廣仁、佐藤晋、佐伯登
    • Industrial Property Rights Holder
      神野雅文、本村英樹、橘邦英、沖廣仁、佐藤晋、佐伯登
    • Industrial Property Number
      2012-134990
    • Filing Date
      2012-06-14
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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