Development of High Performance Gene Transfection Methods Using Microplasma Integrated Devices
| Project/Area Number |
22654070
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plasma science
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| Research Institution | Osaka Electro-Communication University (2011)
Ehime University (2010) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
JINNO Masafumi 愛媛大学, 理工学研究科, 教授 (30274335)
MOTOMURA Hideki 愛媛大学, 理工学研究科, 助教 (80332831)
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| Co-Investigator(Renkei-kenkyūsha) |
HASHIMOTO Koji 愛媛大学, 医学研究科, 教授 (00110784)
SHIRAKATA Yuji 愛媛大学, 医学研究科, 講師 (50226320)
JINNO Masafumi 愛媛大学, 理工学研究科, 教授 (30274335)
MOTOMURA Hideki 愛媛大学, 理工学研究科, 助教 (80332831)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,950,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | 反応性プラズマ / マイクロプラズマ / プラズマメディシン / 遺伝子導入 / プラズマジェット / エレクトロスプレー / GFP遺伝子 / COS細胞 |
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| Research Abstract |
We have tried gene transfection experiments using an integrated device composed of microplasma jets. After the irradiation of plasma jet onto COS cells mixed with GFP genes and cultivation of those cells for 24 hours, the GFP genes have been successfully transfected into a certain amount of cells. Through a series of experiments with several kinds of plasma sources, it is concluded that the effects of transported electric charges from plasma produced particles play a key role in the transfection mechanisms. Upon this understanding, we have developed a new electro-spray method in which a higher efficiency of transfection is achieved with suppressing the damages on living cells.
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Report
(3 results)
Research Products
(13 results)
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[Presentation]2012
Author(s)
H. Motomura, J. Matsuda, K. Ikeda, T. Okihiro, M. Jinno, K. Tachibana, S. Satoh, N. Saeki
Organizer
4th International Conference on Plasma Medicine
Place of Presentation
Orleans, France
Year and Date
2012-06-18
Related Report
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